發(fā)布時間：2019-05-23 11:04

【摘要】：目的建立HPLC法同時測定燈盞細(xì)辛注射液(燈盞細(xì)辛)中5-咖啡酰基奎寧酸、綠原酸、4-咖啡�；鼘幩帷⒖Х人�、1,3-二咖啡酸�？鼘幩帷⒁包S芩苷、異綠原酸B、3,5-二咖啡�；鼘幩帷舯K花甲素、4,5-二咖啡酰基奎寧酸的含有量。方法該藥物水提液的分析采用Inertsil屮ODS-3色譜柱(250 mm×4.6 mm,5μm);流動相0.4%磷酸-乙腈,梯度洗脫;體積流量0.9 m L/min;柱溫30℃;檢測波長327 nm。結(jié)果 10種成分在各自范圍內(nèi)線性關(guān)系良好(r≥0.999 0),平均加樣回收率94.11%~100.64%,RSD 1.12%~2.77%。結(jié)論該方法簡便準(zhǔn)確,重復(fù)性好,可用于燈盞細(xì)辛注射液的質(zhì)量控制。
[Abstract]:Objective to establish a HPLC method for the simultaneous determination of 5-caffeine quinic acid, chlorogenic acid, 4-coffee-quinic acid, caffeine, 1, 3-dicicarboxyquinic acid, baicalin and isochlorogenic acid in Erigeron breviscapus injection (Erigeron breviscapus). 3, the content of 5-di-coffee acyl quinic acid, breviscapine A, 4, 5-di-coffee acyl quinic acid. Methods the water extract of the drug was analyzed by Inertsil ODS-3 column (250 mm 脳 4.6 mm, 5 渭 m), the mobile phase was 0.4% phosphate-acetonitrile, gradient eluting, the volume flow rate was 0.9 mL / min, the column temperature was 30 鈩，

本文編號：2483848