【摘要】：在模擬人體生理?xiàng)l件下,應(yīng)用熒光光譜和表面增強(qiáng)拉曼光譜法研究了紫檀芪(PTE)與人血清白蛋白(HSA)之間相互作用機(jī)制。結(jié)果表明,HSA的熒光能被PTE靜態(tài)猝滅,并伴隨有非輻射能量轉(zhuǎn)移作用,兩者形成了1∶1復(fù)合物,結(jié)合距離r=1.495 nm,結(jié)合常數(shù)KA=1.12×104(298 K)、4.07×104(304 K)和2.45×105L/mol(310 K)。表面增強(qiáng)拉曼光譜研究揭示,PTE分子通過(guò)甲氧基與HSA進(jìn)行結(jié)合;熱力學(xué)數(shù)據(jù)表明,二者間的作用主要為疏水作用;標(biāo)記競(jìng)爭(zhēng)實(shí)驗(yàn)指出PTE優(yōu)先結(jié)合HSA的位點(diǎn)Ⅲ。三維熒光光譜、同步熒光光譜和表面增強(qiáng)拉曼光譜結(jié)果顯示,與PTE作用后,HSA構(gòu)象發(fā)生變化,導(dǎo)致色氨酸殘基周圍環(huán)境疏水性降低,但對(duì)PTE分子構(gòu)象影響不大。
[Abstract]:The interaction between (PTE) and human serum albumin (HSA) was studied by fluorescence spectroscopy and surface-enhanced Raman spectroscopy (SERS) under simulated physiological conditions of human body. The results showed that the fluorescence energy of HSA was quenched by PTE statically and accompanied by non-radiative energy transfer. The 1:1 complex was formed with the binding distance of 1.495 nm, binding constant KA=1.12 脳 10 4 (298 K),). 4.07 x 104 (304K) and 2.45 脳 105L/mol (310 K).) Surface-enhanced Raman spectroscopy (SERS) studies revealed that PTE molecules bind to HSA through methoxy groups; thermodynamic data show that the interaction between the two molecules is mainly hydrophobic; labeling competition experiments indicate that PTE binds HSA preferentially to site 鈪，

本文編號(hào)：2469978