發(fā)布時間：2019-04-24 18:19

【摘要】：毛細(xì)管電泳-質(zhì)譜(CE-MS)聯(lián)用技術(shù)是在液相色譜-質(zhì)譜聯(lián)用技術(shù)基礎(chǔ)上發(fā)展起來的一項新型分析技術(shù),它結(jié)合了毛細(xì)管電泳具有的分離效率高、分離速度快、樣品消耗量少以及質(zhì)譜檢測具有的高靈敏度和強結(jié)構(gòu)解析能力等優(yōu)點,現(xiàn)已成為倍受分析化學(xué)工作者關(guān)注的新型微量分析技術(shù)。目前,CE-MS聯(lián)用技術(shù)是中藥有效成分分析,體內(nèi)藥物分析以及生物樣品,如氨基酸、多肽、蛋白質(zhì)和多糖等分析的重要手段。本文對CE-MS聯(lián)用技術(shù)中同軸鞘流及無鞘流納流電噴霧等幾種接口裝置的研究進展,CE-MS技術(shù)在中藥活性成分分析及多級質(zhì)譜結(jié)構(gòu)解析以及氨基酸、多肽及蛋白質(zhì)等生物樣品分析中的應(yīng)用研究進行了綜述,并對該技術(shù)的發(fā)展進行了展望。
[Abstract]:Capillary electrophoresis-mass spectrometry (CE-MS) is a new analytical technique developed on the basis of liquid chromatography-mass spectrometry (LC-MS). It combines capillary electrophoresis with high separation efficiency and high separation speed. With the advantages of low sample consumption and high sensitivity and strong structure analysis ability of mass spectrometry, it has become a new micro-analysis technology which has been paid close attention to by analytical chemists. At present, CE-MS is an important method for the analysis of active components of traditional Chinese medicine, drug analysis in vivo and analysis of biological samples, such as amino acids, peptides, proteins and polysaccharides. In this paper, several kinds of interface devices, such as coaxial current and unsheathed current electrospray, have been studied in CE-MS combined technique. CE-MS technique has been used in the analysis of active components of traditional Chinese medicine, the structure analysis of multistage mass spectrometry and amino acids. The application of peptides and proteins in the analysis of biological samples was reviewed, and the development of this technique was prospected.
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本文編號：2464676