【摘要】：采用高效液相色譜-離子阱-飛行時(shí)間質(zhì)譜對飲料中生物堿、皂苷和有機(jī)酸等24種植物源性成分進(jìn)行快速篩查、定性識(shí)別和準(zhǔn)確定量。樣品經(jīng)高速離心,過0.22μm水系濾膜后,以C_(18)色譜柱(2.1 mm×250 mm,3.0μm)分離,乙腈-0.1%甲酸溶液為流動(dòng)相進(jìn)行梯度洗脫,正、負(fù)離子模式同時(shí)掃描。結(jié)果表明,24種化合物在50~750μg/L范圍內(nèi)呈良好的線性關(guān)系,相關(guān)系數(shù)均大于0.995。樣品中各化合物的檢出限(以信噪比不小于3計(jì))為8~20μg/L。在3種添加量(70、250、700μg/L)條件下,其平均回收率為63.0%~126.6%,相對標(biāo)準(zhǔn)偏差為4.11%~14.73%。該方法利用精確質(zhì)量數(shù)匹配和自建標(biāo)準(zhǔn)庫檢索,實(shí)現(xiàn)快速篩查,并使用多級(jí)特征碎片離子進(jìn)行確證,具有快速、高效、準(zhǔn)確等優(yōu)點(diǎn),適用于飲料中多種植物源性成分的快速篩查和測定。
[Abstract]:High performance liquid chromatography-ion trap-time-of-flight mass spectrometry was used to screen 24 plant-derived components, such as alkaloids, saponins and organic acids, for qualitative identification and accurate quantification. The samples were centrifuged at high speed and filtered through 0.22 渭 m water system membrane. The samples were separated on a C18 column (2.1 mm 脳 250 mm, 3.0 渭 m). Acetonitrile-0.1% formic acid solution was used as the mobile phase for gradient elution, and the positive and negative ion modes were simultaneously scanned. The results showed that there was a good linear relationship between 50 渭 g / L and 750 渭 g / L for 24 compounds, and the correlation coefficients were more than 0.995. The detection limit of each compound in the sample is 8 ~ 20 渭 g / L. The average recovery was 63.0% ~ 126.6% and the relative standard deviation (RSD) was 4.11% ~ 14.73% under the condition of 3 kinds of additives (70250700 渭 g / L). This method uses accurate mass number matching and self-built standard library retrieval to realize rapid screening, and uses multi-level characteristic fragment ions to confirm it. This method has the advantages of rapid, high efficiency and accuracy, and has the advantages of high speed, high efficiency and accuracy. It is suitable for rapid screening and determination of a variety of plant-derived ingredients in beverages.
【作者單位】： 河北醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院;河北省食品檢驗(yàn)研究院河北省食品安全重點(diǎn)實(shí)驗(yàn)室;北京食品科學(xué)研究院中國肉類食品綜合研究中心;中國標(biāo)準(zhǔn)化研究院;
【基金】：“十二五”國家科技支撐計(jì)劃項(xiàng)目(2015BAK36B03) “十三五”國家重點(diǎn)研發(fā)計(jì)劃重點(diǎn)專項(xiàng)(2016YFF0202300) 國家重大科學(xué)儀器設(shè)備開發(fā)專項(xiàng)(2012YQ03011110) 河北省高等學(xué)校優(yōu)秀青年基金項(xiàng)目(YQ2013018)
【分類號(hào)】：O657.63;TS27
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本文編號(hào)：2442675