【摘要】：以矢車菊素、兒茶素、表兒茶素、表兒茶素沒食子酸酯為標準品,采用島津LC-20A高效液相色譜儀、二極管陣列檢測器,建立慕薩萊思酒中原花青素含量的HPLC測定方法。優(yōu)化的色譜條件為:Inertsil DOS-3,5μm 4.6 mm×250 mm色譜柱;流動相A為甲醇,流動相B為超純水,流動相比例:流動相A為35%,流動相B為65%;流速1.0 m L/min;進樣量10μL;柱溫38℃。采用建立的HPLC方法對慕薩萊思酒進行分析,出峰效果良好;標準曲線正常,回收率為82%~93%。本方法操作簡單、準確度高、測定時間短,可用于慕薩萊思酒中原花青素的測定。
[Abstract]:A HPLC method for the determination of proanthocyanidins in Musalez wine was established by using LC-20A high performance liquid chromatograph and diode array detector with cortisone catechin and epigallocatechin gallate as standard. The optimized chromatographic conditions were as follows: Inertsil DOS-3,5 渭 m 4.6 mm 脳 250 mm column, mobile phase A was methanol, mobile phase B was ultrapure water, mobile phase ratio was: mobile phase A was 35g, mobile phase B was 65%, flow rate was 1.0 mL / min, sample volume was 10 渭 L. The column temperature is 38 鈩，

本文編號：2406372