【摘要】：在中藥治療過程中,以方劑為載體,注重整體,采用辨證論治的方法。為揭示方劑的藥隊(duì)規(guī)律,根據(jù)藥隊(duì)協(xié)同效應(yīng)設(shè)計(jì)法,將活血化瘀湯方劑拆分為"調(diào)和"、"化瘀生新"、"活血止痛"、"補(bǔ)氣"四個(gè)藥隊(duì)方劑。由于銀納米粒子可與煎劑產(chǎn)生強(qiáng)交互作用,并極大地增強(qiáng)拉曼信號,獲取活血化瘀湯、調(diào)和藥隊(duì)煎劑、化瘀生新藥隊(duì)煎劑、活血止痛藥隊(duì)煎劑和補(bǔ)氣藥隊(duì)煎劑五種方劑煎劑的表面增強(qiáng)拉曼散射(SERS)光譜,并對所有SERS譜峰進(jìn)行歸屬。主要針對各煎劑中的18個(gè)拉曼信號(523,538,622,647,732,959,977,1 003,1 048,1 077,1 145,1 245,1 326,1 402,1 456,1 470,1 518和1 605cm~(-1))進(jìn)行討論�；钛鰷玈ERS光譜中保留了藥隊(duì)煎劑的某些拉曼峰,如:538,622,647,732,959,1 003,1 048,1 326,1 402,1 456,1 470,1 518和1 605cm~(-1)。藥隊(duì)煎劑SERS光譜的某些拉曼峰并未在活血化瘀湯中出現(xiàn),如:1 077,1 145和1 245cm~(-1)。在活血化瘀湯SERS光譜中,出現(xiàn)了新的拉曼峰,如:523和977cm~(-1)。新的拉曼峰出現(xiàn)說明新物質(zhì)的產(chǎn)生。實(shí)驗(yàn)結(jié)果表明,活血化瘀湯所包含的藥物成分并非是各藥隊(duì)煎劑所含藥物成分的簡單相加,SERS光譜可為方劑藥隊(duì)規(guī)律研究提供一優(yōu)異、有效和準(zhǔn)確的檢測方法。
[Abstract]:In the course of traditional Chinese medicine treatment, the prescription is used as the carrier, paying attention to the whole and adopting the method of differentiation of symptoms and signs. In order to reveal the rule of prescription, according to the design method of synergistic effect, the prescription of Huoxue Huayu Tang was divided into four prescriptions: "Harmony", "Huayu Shengxin", "Huoxue Zhitong", "tonifying Qi". Because silver nanoparticles can produce strong interaction with the decoction, and greatly enhance the Raman signal, get Huoxue Huayu decoction, reconcile the decoction of the medicinal team, and remove the blood stasis of the new drug team decoction. The surface enhanced Raman scattering (SERS) spectra of the five prescriptions of the decoction for promoting blood circulation and analgesic drugs and the decoction for tonifying qi were studied, and all the peaks of SERS spectrum were assigned. In this paper, 18 Raman signals in each decoction (523 ~ 538 ~ 622647 ~ 1 4701 ~ (-1) and 1 605cm ~ (-1) were discussed. The Raman signals (523 ~ 5388 ~ 622 ~ 959 ~ 997 ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) were discussed. Some Raman peaks of Huoxue Huayu decoction (Huoxue Huayu decoction) were preserved in the SERS spectra, such as 538CX 6222A647C 732N 959C 1 003C 1 048C 1 326C 1 402N 1 456U 1 470518 and 1 605cm ~ (-1). The results showed that the Raman peaks of Huoxue Huayu decoction (Huoxue Huayu decoction) were 1 4701 518 and 1 605cm ~ (-1) respectively. Some Raman peaks in the SERS spectrum of the decoction were not found in Huoxue Huayu decoction, such as 1 077C 1 145 and 1 245 cm ~ (-1). New Raman peaks such as 523 and 977 cm-1 appeared in the SERS spectra of Huoxue Huayu Tang. The appearance of new Raman peaks indicates the production of new substances. The experimental results showed that the components contained in Huoxue Huayu decoction were not the simple additive of the components contained in the decoction, and the SERS spectrum could provide an excellent, effective and accurate method for the study of the regularity of the prescriptions.
【作者單位】： 福建中醫(yī)藥大學(xué)中西醫(yī)結(jié)合學(xué)院;福建中醫(yī)藥大學(xué)骨傷學(xué)院;醫(yī)學(xué)光電科學(xué)與技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室(福建師范大學(xué));
【基金】：國家自然科學(xué)基金項(xiàng)目(61405036,61210016,61178090,81503600) 福建省自然科學(xué)基金項(xiàng)目(2017J01499,2017J01843,2017J01844) 福建省教育廳科技項(xiàng)目(JA15233)資助
【分類號】：R285;O657.37

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