【摘要】：21世紀以來,使用化石能源對環(huán)境造成的污染愈發(fā)嚴重,尋找可再生的替代能源愈發(fā)迫切。秸稈含有大量的木質(zhì)纖維素,是自然界中含量最多的可再生資源,合理利用這種可再生資源成為人們關(guān)注的熱點。黃孢原毛平革菌作為一種降解木質(zhì)素能力最強的真菌被廣為研究,含有黃孢的混合菌劑因其具有較之黃孢更為強大的降解能力成為研究的重點。綠色木霉是一種廣泛存在于自然界中的木質(zhì)素降解菌,因其與黃孢搭配具有較強的木質(zhì)素降解能力被認為是一種理想的堆肥接種菌。本研究以稻草粉末培養(yǎng)基為依托,利用頂空固態(tài)微萃取等技術(shù),對黃孢和綠色木霉的純培養(yǎng)及其混合培養(yǎng)進行了研究,重點研究了混合培養(yǎng)中同一平板接觸區(qū)域與非接觸區(qū)域的各種差異,以期對混合培養(yǎng)中黃孢和綠色木霉降解木質(zhì)素的機制有更深入的了解。本研究主要對黃孢和綠色木霉的純培養(yǎng)及其混合培養(yǎng)各區(qū)域的揮發(fā)性有機物、草酸含量、木質(zhì)素過氧化物酶活性和p H進行了檢測,就其數(shù)據(jù)進行了比對分析。結(jié)果表明:兩株菌的混合培養(yǎng)較其純培養(yǎng)有更強的降解稻草的能力。在混合培養(yǎng)過程中檢測到C15H24的兩種同分異構(gòu)體,分別屬于雙環(huán)倍半水芹烯和環(huán)己二烯類物質(zhì),均是倍半萜烯,證實了黃孢原毛平革菌和綠色木霉混合培養(yǎng)時的拮抗機制。環(huán)己二烯類物質(zhì)僅在混合培養(yǎng)中的混合區(qū)域檢測到,表明混合培養(yǎng)中混合區(qū)域較之非混合區(qū)域具有更強的競爭表現(xiàn),分區(qū)域檢測降解過程中揮發(fā)性有機物能更好的研究混合接種的降解機理。草酸的分泌可能是黃孢降解木質(zhì)纖維素能力強于綠色木霉的原因之一;混合培養(yǎng)中各區(qū)域草酸含量變化由于受到綠色木霉的干擾總體呈下降趨勢,說明真菌間的拮抗作用對真菌分泌草酸的量有較大影響。黃孢和綠色木霉純培養(yǎng)狀態(tài)下均能產(chǎn)木質(zhì)素過氧化物酶,但黃孢產(chǎn)酶能力強于綠色木霉是黃孢降解木質(zhì)纖維素能力高于綠色木霉的另一個原因;草酸是影響培養(yǎng)體系中p H的重要因素,其變化趨勢與草酸的分泌含量呈負相關(guān);但競爭導致混合培養(yǎng)中不再呈負相關(guān)。
[Abstract]:Since the 21st century, the environment pollution caused by fossil energy is becoming more and more serious, so it is more urgent to find renewable alternative energy. Straw contains a large amount of lignocellulose, which is the most abundant renewable resource in nature. The rational utilization of this renewable resource has become the focus of attention. As a kind of fungus with the strongest lignin degradation ability, P. xanthosporium has been widely studied, and the mixed fungicides containing xanthosporins have become the focus of research because of their more powerful degradation ability than that of xanthosporium. Trichoderma viride (Trichoderma viride) is a kind of lignin degrading bacteria widely existing in nature. Because of its strong lignin degradation ability with xanthosporium, Trichoderma viride is considered to be an ideal compost inoculation bacteria. The pure culture and mixed culture of Trichoderma viride and Trichoderma viridis were studied by headspace solid microextraction based on rice straw powder medium. In order to understand the mechanism of lignin degradation by Trichoderma viride and Trichoderma viride in mixed culture, the differences between the contact region of the same flat plate and the non-contact area were studied. In this study, volatile organic compounds (VOCs), oxalic acid (oxalic acid), lignin peroxidase (lignin) activity and pH were detected in pure culture and mixed culture of Trichoderma viridis and Trichoderma viridis, and the data were compared and analyzed. The results showed that the mixed culture of the two strains had stronger ability to degrade rice straw than its pure culture. Two isomers of C15H24, belonging to bicyclic hemihydrate and cyclohexadiene, were detected in mixed culture, which were sesquiterpene, which confirmed the antagonistic mechanism of P. xanthosporium and Trichoderma viridis in mixed culture. Cyclohexadienes were detected only in the mixed region of mixed culture, which indicated that the mixed region had stronger competitive performance than the non-mixed region in mixed culture. The degradation mechanism of mixed inoculation can be better studied by detecting volatile organic compounds in the process of degradation. The secretion of oxalic acid may be one of the reasons why xanthosporium can degrade lignocellulose better than Trichoderma viridis. The change of oxalic acid content in different regions of mixed culture showed a decreasing trend because of the interference of Trichoderma viridis, which indicated that the antagonism between fungi had a great effect on the amount of oxalic acid secreted by fungi. Both yellow spores and Trichoderma viridis could produce lignin peroxidase in pure culture, but the ability of xanthosporins to produce lignin peroxidase was stronger than that of Trichoderma viridis, which was another reason that the ability of degradation of lignocellulose by xanthosporium was higher than that of Trichoderma green. Oxalic acid is an important factor affecting pH in culture system, and its changing trend is negatively correlated with oxalic acid secretion, but competition leads to no negative correlation in mixed culture.
【學位授予單位】：湖南大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：TQ914.3;O636.2

【相似文獻】

相關(guān)期刊論文 前10條

1 褚福紅;于新;;綠色木霉代謝物抑菌活性及其穩(wěn)定性的研究[J];食品工業(yè);2011年05期

2 劉芳;楊佳穎;陳紅漫;;綠色木霉外源基因表達系統(tǒng)的構(gòu)建[J];湖北農(nóng)業(yè)科學;2013年13期

3 王儀明;張宗舟;藺海明;孫小弟;雷艷芳;王東明;;綠色木霉固態(tài)發(fā)酵產(chǎn)纖維素酶活力的研究[J];草業(yè)科學;2009年05期

4 劉靖;劉萍;夏文水;高勤學;;不同碳源誘導綠色木霉產(chǎn)雙功能酶的表達差異分析[J];食品與機械;2011年05期

5 藍舟琳;彭丹;郭楚玲;朱超飛;薛秀玲;黨志;;綠色木霉改性玉米秸稈溢油吸附劑的制備及其性能研究[J];環(huán)境科學;2013年04期

6 邵喜霞;韓大勇;張力;;綠色木霉合成纖維素酶部分培養(yǎng)條件的優(yōu)化[J];安徽農(nóng)業(yè)科學;2009年23期

7 鄭穎潔;余勃;陸豫;;綠色木霉降解稻草秸桿產(chǎn)葡萄糖工藝優(yōu)化研究[J];安徽農(nóng)業(yè)科學;2010年13期

8 陳光;徐楊;孫e，

本文編號：2387774