【摘要】：研究質(zhì)譜特征離子對(duì)海洋魚(yú)油脂肪酸的快速定性分析。首先,根據(jù)脂肪酸甲酯的斷裂規(guī)律,基峰離子可快速確定脂肪酸的不飽和度,如基峰離子m/z 74為飽和脂肪酸,m/z 55為單烯脂肪酸,m/z 67為二烯脂肪酸,m/z 79為三烯及以上多不飽和脂肪酸;其次,分子離子M+及其特征性碎片丟失而形成的特征離子如[M-29]~+、[M-31]~+、[M-32]~+、[M-43]~+和[M-74]~+等,可有效用于脂肪酸甲酯分子量和脂肪酸碳鏈長(zhǎng)度的分析;而ω離子和α離子可準(zhǔn)確對(duì)三烯及以上多不飽和脂肪酸中的雙鍵位置進(jìn)行分析鑒定。通過(guò)質(zhì)譜特征離子的綜合分析,從海洋魚(yú)油膠囊中共分析鑒定出24種脂肪酸,以C_(14:0)、C_(16:0)、C_(18:1n-9)、C_(20:5n-3)和C_(22:6n-3)為主,表明質(zhì)譜特征離子能快速準(zhǔn)確用于海洋魚(yú)油脂肪酸的定性分析。同時(shí),海洋魚(yú)油中C_(20:5n-3)和C_(22:6n-3)的總含量高達(dá)29.13%,表明其具有很高的營(yíng)養(yǎng)價(jià)值和保健功能。
[Abstract]:The rapid qualitative analysis of fatty acids in marine fish oil by characteristic ions of mass spectrometry was studied. First of all, according to the breaking rule of fatty acid methyl ester, the base ion can quickly determine the unsaturated fatty acid, such as base ion m / z 74 is saturated fatty acid, m / z 55 is monene fatty acid, m / z 67 is diene fatty acid, M / z 79 is triene and above polyunsaturated fatty acids; Secondly, molecular ions M and their characteristic fragments are lost, such as [M-29] ~, [M-31] ~, [M-32] ~, [M-43] ~ and [M-74] ~, etc. It can be used to analyze the molecular weight of fatty acid methyl ester and the length of fatty acid carbon chain. However, 蠅 ion and 偽 ion can accurately analyze and identify the double bond positions in triene and above polyunsaturated fatty acids. A total of 24 fatty acids were identified from marine fish oil capsules by comprehensive analysis of characteristic ions by mass spectrometry, using C _ (14:0), C _ (16:0), C _ (18:1n-9). C _ (20:5n-3) and C _ (22:6n-3) were dominant, which indicated that the characteristic ions of mass spectrometry could be applied to the qualitative analysis of fatty acids in marine fish oil quickly and accurately. At the same time, the total contents of C _ (20:5n-3) and C _ (22:6n-3) in marine fish oil were as high as 29.13, which indicated that they had high nutritional value and health care function.
【作者單位】： 寧波大學(xué)海洋學(xué)院;中國(guó)海洋大學(xué)食品科學(xué)與工程學(xué)院;中國(guó)水產(chǎn)科學(xué)研究院南海水產(chǎn)研究所農(nóng)業(yè)部水產(chǎn)品加工重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31201283) “水產(chǎn)”浙江省重中之重學(xué)科開(kāi)放基金項(xiàng)目(xkzsc1531) 農(nóng)業(yè)部水產(chǎn)品加工重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金項(xiàng)目(nybjg201208)
【分類號(hào)】：O657.63;TS225.24

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