發(fā)布時(shí)間：2018-12-14 08:25

【摘要】：目的通過(guò)超高效液相色譜-四級(jí)桿-飛行時(shí)間質(zhì)譜(UPLC-Q-TOF-MS)法分析雙丹明目膠囊化學(xué)成分。方法該膠囊內(nèi)容物的分析采用Agilent Extend C18色譜柱(2.1 mm×50 mm,1.8μm);流動(dòng)相0.1%甲酸(A)-乙腈(B),梯度洗脫;體積流量0.4 m L/min。根據(jù)對(duì)照品的相對(duì)分子質(zhì)量、質(zhì)譜碎片離子信息,結(jié)合參考文獻(xiàn)對(duì)成分進(jìn)行鑒定。結(jié)果從中共鑒定出102種成分,包括酚酸、黃酮及其苷類(lèi)、萜類(lèi)、香豆素、木脂素等。其中,24種來(lái)自旱墨蓮,13種來(lái)自女貞子,11種來(lái)自丹參,14種來(lái)自澤瀉,9種來(lái)自山茱萸,9種來(lái)自茯苓,6種來(lái)自牛膝,6種來(lái)自三七,4種來(lái)自牡丹皮,3種來(lái)自紅土茯苓,3種來(lái)自山藥。結(jié)論該方法靈敏、可靠、快速,可為雙丹明目膠囊藥效物質(zhì)基礎(chǔ)及質(zhì)量控制研究提供依據(jù)。
[Abstract]:Objective to analyze the chemical constituents of Shuangdan Mingmu capsule by ultra-high performance liquid chromatography-4-step-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Methods Agilent Extend C18 column (2.1 mm 脳 50 mm,1.8 渭 m);) was used to analyze the contents of the capsule. The mobile phase of 0.1% formic acid (A) acetonitrile (B), was gradient eluted, and the volume flow rate was 0.4 mL / min. The components were identified according to the relative molecular weight of the reference substance and the information of fragment ion in mass spectrometry. Results 102 compounds were identified, including phenolic acid, flavonoids and their glycosides, terpenes, coumarins, lignans, etc. Of these, 24 were from Artemisia, 13 from Ligustrum lucidum, 11 from Salvia miltiorrhiza, 14 from alisma, 9 from Fructus Corni, 9 from Poria cocos, 6 from Achyranthes bidentata, 6 from Panax Notoginseng, and 4 from peony bark. Three species came from Rhizoma Poria cocos and three from Chinese yam. Conclusion the method is sensitive, reliable and rapid, and can provide basis for the study of the pharmacodynamics and quality control of Shuangdan Mingmu capsule.
【作者單位】： 湖南中醫(yī)藥大學(xué)第一附屬醫(yī)院;湖南省中藥粉體與創(chuàng)新藥物省部共建國(guó)家重點(diǎn)實(shí)驗(yàn)室培育基地;湖南中醫(yī)藥大學(xué);
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81473737)
【分類(lèi)號(hào)】：O657.63;R286.0
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本文編號(hào)：2378284