【摘要】：第一章:主要介紹了熒光分析法的基本原理,熒光探針的結構及其響應機理,并且對久洛尼定熒光探針的研究進展做了簡要概述。第二章:以8-羥基久洛尼定-9-甲醛和氨基脲為原料合成了一種新型的可逆性比色熒光希夫堿探針(L),該探針可檢測緩沖水體系中的Al~(3+)離子。加入Al~(3+)后,L的熒光增強,伴隨著溶液顏色由無色變成黃色,基于此可識別檢測溶液中的Al~(3+)。等摩爾連續(xù)變化法和高分辨質譜實驗結果表明,L與Al~(3+)以1:1結合形成新的絡合物。實驗測得Al~(3+)的檢測限為4.67nM,遠低于WHO規(guī)定的飲用水中Al~(3+)最大含量(7.41μM)。此外,L還可通過熒光共聚焦成像用于活體細胞中Al~(3+)離子的識別檢測。第三章:設計合成了一種以8-羥基久洛尼定-9-甲醛與4-苯基氨基脲為原料的希夫堿熒光探針L,并對其光學特性進行了研究。在甲醇-水(4:1,v/v,HEPES,pH=7.4)緩沖溶液中,L以1:1與Cu~(2+)特異性結合,540nm處的熒光猝滅,同時溶液從綠色變?yōu)辄S色。另外,形成的L-Cu~(2+)絡合物可以通過金屬置換法,進一步用于氰離子的檢測。向L-Cu~(2+)中加入CN-,L熒光恢復,且絡合物對CN-有較高的選擇性。探針對Cu~(2+)和CN-的檢測限分別為2.28×10-7M和1.33×10-8M。該傳感器已應用于實際水樣中CN-的檢測,性能優(yōu)良。此外,L還進行了活細胞中的熒光成像實驗,表明其可用來檢測活細胞中的Cu~(2+)和CN-。第四章:設計并合成了一種以8-羥基久洛尼定-9-甲醛和4-苯基氨基硫脲為原料的新型希夫堿熒光探針。該探針可以作為一個多功能熒光探針同時檢測Al~(3+)和Cu~(2+)。在甲醇溶劑中,向L中加入Al~(3+)后,溶液顏色從黃色變?yōu)槌壬?并且熒光發(fā)射波長發(fā)生紅移,從540nm移至570nm,熒光顏色由黃綠色變?yōu)榉凵�。通過等摩爾連續(xù)變化法測得L與Al~(3+)按1:1結合,計算得L對Al~(3+)的檢測限為6.38×10-8M,遠低于WHO規(guī)定的飲用水中Al~(3+)最高含量(7.41μM);另外,在乙腈-水(1:1,v/v,HEPES,pH7.4)緩沖溶液中,L可以選擇性地檢測Cu~(2+)。加入Cu~(2+)之后,L溶液的顏色由黃色變?yōu)樽睾稚?同時熒光猝滅,由實驗測得L對Cu~(2+)的檢測限為6.7×10-8M,遠低于USEPA對飲用水中Cu~(2+)含量的規(guī)定。此外,L也可成功地檢測實際水樣中的Cu~(2+)。
[Abstract]:Chapter 1: the basic principle of fluorescence analysis, the structure of fluorescent probe and its response mechanism are introduced, and the research progress of the fluorescent probe is briefly summarized. Chapter 2: a novel reversibility colorimetric fluorescence Schiff base probe (L), was synthesized from 8-hydroxy-goulonidine-9-formaldehyde and semicarbazone. The probe can be used to detect Al~ (3) ions in buffer water system. After the addition of Al~ (3), the fluorescence of L was enhanced, and the color of the solution changed from colorless to yellow. Based on this, the Al~ (3) in the solution could be identified and detected. The results of constant molar variation method and high resolution mass spectrometry show that L combines with Al~ (3) at 1:1 to form a new complex. The detection limit of Al~ (3) was 4.67 nm, which was far lower than the maximum content of Al~ (3) in drinking water specified by WHO (7.41 渭 M). In addition, L can be used to detect Al~ (3) ions in living cells by fluorescence confocal imaging. In chapter 3, a Schiff base fluorescence probe L- (Schiff base) was designed and synthesized using 8-hydroxy-goulonidine-9-formaldehyde and 4-phenylsemicarbazone as raw materials, and its optical properties were studied. In methanol-water (4: 1 v / v) buffer solution, L binds to Cu~ (2) at 1:1, quenching the fluorescence at 540nm, and the solution changes from green to yellow. In addition, the formed L-Cu2 complex can be further used for the detection of cyanide ions by metal replacement method. CN-,L was added to L-Cu2 to restore fluorescence, and the complex was highly selective to CN-. The detection limits of Cu~ (2) and CN- were 2.28 脳 10 ~ (-7) M and 1.33 脳 10 ~ (-8) M, respectively. The sensor has been applied to the detection of CN- in real water samples and has good performance. In addition, L has carried out fluorescence imaging experiments in living cells, indicating that it can be used to detect Cu~ (2) and CN-. in living cells. In chapter 4, a novel Schiff base fluorescence probe was designed and synthesized from 8-hydroxy iuronidine-9-formaldehyde and 4-phenylthiosemicarbazone. The probe can be used as a multifunctional fluorescent probe for simultaneous detection of Al~ (3) and Cu~ (2). When Al~ (3) was added to L in methanol solvent, the color of the solution changed from yellow to orange, and the fluorescence emission wavelength shifted red from 540nm to 570 nm, and the fluorescence color changed from yellow green to pink. The detection limit of L for Al~ (3) is 6.38 脳 10 ~ (-8) M, which is far lower than the maximum Al~ (3) content (7.41 渭 M);) in drinking water specified by WHO. The combination of L and Al~ (3) is determined by the method of constant molar continuous variation at 1:1, and the detection limit of L for Al~ (3) is 6.38 脳 10 ~ (-8) m. In addition, in acetonitrile-water (1: 1 v / v) buffer solution, L can selectively detect Cu~ (2) in pH 7.4. After the addition of Cu~ (2), the color of L solution changed from yellow to brown, and the fluorescence quenching. The detection limit of L for Cu~ (2) was 6.7 脳 10 ~ (-8) m, which was much lower than that stipulated by USEPA for Cu~ (2) in drinking water. In addition, L can also be successfully used to detect Cu~ (2) in real water samples.
【學位授予單位】：山西大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3

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1 胡睿;郭旭東;楊國強;;激發(fā)態(tài)分子內質子轉移化合物的性能及作為熒光化學傳感器的應用研究[J];影像科學與光化學;2013年05期

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