【摘要】：建立半枝蓮藥材中野黃芩苷、黃芩素、木犀草素和芹菜素含量同時(shí)測(cè)定的HPLC方法。色譜條件為DiamonsilC18柱(250 mm×4.6 mm,5μm)色譜柱分離,檢測(cè)波長(zhǎng)為335 nm,流動(dòng)相為乙腈(A)-1%冰醋酸水溶液(B)進(jìn)行梯度洗脫,流速為1.0 m L/min,柱溫為30℃。野黃芩苷、黃芩素、木犀草素和芹菜素濃度分別在2.0~200、0.5~50、0.5~50、0.5~50μg/m L范圍內(nèi)與峰面積積分值線性關(guān)系良好。在精密度實(shí)驗(yàn)、穩(wěn)定性實(shí)驗(yàn)、重復(fù)性實(shí)驗(yàn)中,野黃芩苷、黃芩素、木犀草素和芹菜素峰面積的相對(duì)標(biāo)準(zhǔn)偏差(RSD)2.0%;野黃芩苷、黃芩素、木犀草素和芹菜素的加樣回收率范圍及平均值和RSD分別為97.73%~101.7%、99.72%、1.36%(n=9),97.78%~102.8%、99.53%、1.99%(n=9),97.40%~102.4%、99.39%、1.84%(n=9)和97.80%~102.0%、99.24%、1.49%(n=9)。所測(cè)12份不同產(chǎn)地半枝蓮藥材中,野黃芩苷、黃芩素、木犀草素和芹菜素的含量范圍分別為2.41~7.08、0.26~0.56、0.31~0.90、0.28~0.95 mg/g。本方法具有良好的專屬性和重現(xiàn)性,加樣回收率實(shí)驗(yàn)符合要求,能準(zhǔn)確、穩(wěn)定地對(duì)半枝蓮藥材中野黃芩苷、黃芩素、木犀草素和芹菜素含量進(jìn)行同時(shí)定量測(cè)定。
[Abstract]:A HPLC method for simultaneous determination of baicalin, luteolin and apigenin in Scutellaria barbata was established. The chromatographic conditions were as follows: DiamonsilC18 column (250 mm 脳 4.6 mm,5 渭 m) column, the mobile phase at 335 nm, was gradient eluted by acetonitrile (A) 1% glacial acetic acid solution (B), the flow rate was 1.0 mL / min, and the column temperature was 30 鈩，

本文編號(hào)：2339502