【摘要】：目的:建立同時(shí)測(cè)定舒肝寧注射液中5種成分含量的方法。方法:采用高效液相色譜法。色譜柱為Symmetry~? C_(18),流動(dòng)相為甲醇-0.4%磷酸溶液(梯度洗脫),流速為1.0 mL/min,檢測(cè)波長(zhǎng)為238 nm(梔子苷、黃芩苷)、327 nm(綠原酸、野黃芩苷、黃芩素),柱溫為30℃,進(jìn)樣量為10μL。結(jié)果:綠原酸、梔子苷、黃芩苷、黃芩素、野黃芩苷檢測(cè)質(zhì)量濃度線性范圍分別為0.406 2~26.0μg/mL(r=0.999 9)、2.500 0~160.0μg/mL(r=0.999 9)、6.562 0~420.0μg/mL(r=0.999 9)、0.312 5~20.0μg/mL(r=0.9996)、0.585 9~37.5μg/mL(r=0.999 8);定量限≤31.20 ng,檢測(cè)限≤15.60 ng;精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD2.0%;加樣回收率分別為97.72%~101.10%(RSD=1.21%,n=6)、97.67%~102.40%(RSD=1.87%,n=6)、97.64%~101.10%(RSD=1.31%,n=6)、96.45%~100.10%(RSD=1.47%,n=6)、96.16%~101.10%(RSD=1.69%,n=6)。結(jié)論:該方法操作簡(jiǎn)便,精密度、穩(wěn)定性、重復(fù)性好,可用于舒肝寧注射液中5種成分含量的同時(shí)測(cè)定。
[Abstract]:Objective: to establish a method for simultaneous determination of five components in Shuganning injection. Methods: high performance liquid chromatography was used. Chromatographic column Symmetry~? C _ (18), mobile phase consisted of methanol-0.4% phosphoric acid solution (gradient elution), flow rate was 1.0 mL/min, detection wavelength was 238 nm (geniposide, baicalin), 327 nm (Lv Yuan, baicalin, baicalin). The column temperature is 30 鈩，

本文編號(hào)：2335236