【摘要】：氨基酸是組成蛋白質(zhì)的基本單元,在人體及動物生命活動中起著舉足輕重的作用。光學(xué)純氨基酸是合成多肽和內(nèi)酰胺類抗生素等藥物的重要中間體,在藥物合成、新材料開發(fā)、食品添加劑和精細化學(xué)品的研發(fā)等方面都具有巨大的應(yīng)用價值。近年來,D-氨基酸的重要生理機能(作為疾病標志物等)研究已逐漸成為作用于神經(jīng)及內(nèi)分泌系統(tǒng)的新型藥物開發(fā)或疾病新診斷方法研發(fā)等領(lǐng)域的熱點。但是在生物樣品中往往存在大量的L-氨基酸,不利于體內(nèi)微量D-氨基酸的分析研究,因此開發(fā)有效的氨基酸手性拆分方法意義重大。在常見的手性拆分方法中,手性固定相(CSP)色譜法因具有準確、快速及固定相選擇范圍寬等特點,現(xiàn)已在液相色譜等領(lǐng)域得到了廣泛應(yīng)用。以氨基酸及金雞納類等為手性選擇劑的"刷型"手性固定相是D-氨基酸液相色譜分析中非常重要的一類手性固定相,同時也是手性液相色譜領(lǐng)域使用量大,適用面廣,對手性識別機理揭示較深的一類固定相。它的識別是基于手性分子和固定相之間的氫鍵作用、π-π作用、偶極堆積作用等實現(xiàn)的。針對近年來"刷型"手性固定相的快速發(fā)展,本文詳盡地介紹了目前常用的Sumichiral OA型、金雞納類(奎尼丁或者奎寧)等手性固定相在D-氨基酸分離分析中的應(yīng)用情況。
[Abstract]:Amino acid is the basic unit of protein, which plays an important role in human and animal life. Optical pure amino acids are important intermediates for synthetic polypeptides and lactam antibiotics, which have great application value in drug synthesis, development of new materials, food additives and fine chemicals. In recent years, the research on the important physiological function of D- amino acid (as a disease marker) has gradually become a hot spot in the field of new drug development or new diagnosis method of disease acting on nerve and endocrine system. However, there are a lot of L- amino acids in biological samples, which is not conducive to the analysis of trace D- amino acids in vivo, so it is of great significance to develop an effective method of chiral resolution of amino acids. Among the common chiral resolution methods, chiral stationary phase (CSP) chromatography has been widely used in liquid chromatography due to its characteristics of accuracy, rapidity and wide range of stationary phase selection. "Brush" chiral stationary phase with amino acids and Jinchona as chiral selectors is a very important chiral stationary phase in D- amino acid liquid chromatography, and it is also widely used in the field of chiral liquid chromatography. Chiral recognition mechanism reveals a deeper class of stationary phases. Its recognition is based on the hydrogen bonding between chiral molecules and stationary phases, 蟺-蟺 interaction and dipole stacking. In view of the rapid development of brush-type chiral stationary phase in recent years, the application of chiral stationary phases, such as Sumichiral OA type, quinidine or quinine, in the separation and analysis of D-amino acids, is introduced in detail in this paper.
【作者單位】： 暨南大學(xué)藥學(xué)院;
【基金】：國家自然科學(xué)基金(81202499) 廣東省省級科技計劃項目(2016A040403055)資助
【分類號】：O629.71;O652

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