發(fā)布時間：2018-11-07 18:36

【摘要】：建立了高效液相色譜-三重四極桿質(zhì)譜(HPLC-MS/MS)測定面粉中7種熒光增白劑(FWAs)的分析方法。在樣品中加入30 mL乙酸乙酯,超聲提取10 min,離心后在40℃下氮吹濃縮,用乙腈定容后,上機(jī)檢測。選用Phenomenex Kinetex C18色譜柱(100 mm×2.1 mm,2.6μm)分離,以0.1%(v/v)甲酸-乙腈和水為流動相進(jìn)行梯度洗脫,采用電噴霧離子(ESI)源,在正離子、多反應(yīng)監(jiān)測(MRM)模式下進(jìn)行掃描,外標(biāo)法定量。7種熒光增白劑在各自范圍內(nèi)線性關(guān)系良好,相關(guān)系數(shù)均大于0.99,檢出限(S/N=3)為0.50~2.65μg/kg,定量限(S/N=10)為1.60~8.80μg/kg;在3個添加水平下的平均加標(biāo)回收率為64.8%~117.5%,相對標(biāo)準(zhǔn)偏差(n=6)為0.9%~14.4%。該方法操作簡單、回收率高、精密度好,適于面粉中7種熒光增白劑的測定。
[Abstract]:A method for the determination of seven fluorescent brighteners (FWAs) in flour by high performance liquid chromatography-quadrupole mass spectrometry (HPLC-MS/MS) was established. Adding 30 mL ethyl acetate to the sample, ultrasonic extraction was centrifuged for 10 min, then concentrated by nitrogen blowing at 40 鈩，

本文編號：2317239