【摘要】：蛇莓(Duchesnea indica(Andr.)Focke),又名地莓等,為薔薇科多年生草本植物,作為傳統(tǒng)的中藥材在《名醫(yī)別錄》和《本草綱目》中均有記載。蛇莓分布廣泛,在中國(guó)尤其是長(zhǎng)江流域資源豐富,在印度,歐洲以及美洲也有分布。蛇莓的用途廣泛,藥用有清熱解毒、散瘀消腫、涼血止血、治療蛇蟲咬傷等功效。本文總結(jié)了近年來(lái)蛇莓的化學(xué)成分和藥理作用的研究成果,發(fā)現(xiàn)在蛇莓的DPPH自由基清除活性和酪氨酸酶抑制活性方面的報(bào)道較少,因此,本文選用這兩種活性為導(dǎo)向,采用MCI柱層析,大孔樹脂柱層析以及ODS-A反相柱層析和高效液相色譜等分離手段,從長(zhǎng)白山區(qū)薔薇科植物蛇莓中分離得到13個(gè)化合物,并對(duì)其中6個(gè)極性化合物的分子結(jié)構(gòu)進(jìn)行結(jié)構(gòu)鑒定,它們分別為3-O-β-D-glucopyranoxy-6-hydroxy-p-cymene(1),沒(méi)食子酸甲酯(Methyl 3,4,5-trih-ydroxybenzoate)(2),黑色五味子單體苷(Isolariciresinol-2α-O-β-D-xyloside)(6),牡荊素(Apigenin-8-C-glucoside)(9),1-羥基松脂醇-1-β-D-葡萄糖苷[(+)-1-Hydroxysyringaresinol-1-β-D-glucoside](10),(6R,9R)-3-氧代-α-紫羅蘭醇-β-D-葡萄糖苷[(6R,9R)-3-Oxo-α-ionol-β-D-glucopyranoside](13)。對(duì)分離并鑒定結(jié)構(gòu)的化合物分別進(jìn)行了 DPPH自由基清除和酪氨酸酶活性抑制實(shí)驗(yàn),結(jié)果顯示,所有化合物的DPPH自由基清除率和酪氨酸酶抑制率均在90%以上,其中化合物10,13的DPPH自由基清除活性IC50值分別為2.23μg/mL、2.07μg/mL,小于陽(yáng)性參展組維生素C。化合物2,13的酪氨酸酶抑制率高于參照組曲酸且化合物13的酪氨酸酶抑制活性IC50值為3.3 μg/mL。
[Abstract]:(Duchesnea indica (Andr.) Focke), is a perennial herb of Rosaceae. As a traditional Chinese herbal medicine, it is recorded in the famous Medical Records and Compendium of Materia Medica (Compendium of Materia Medica). It is widely distributed in China, especially in the Yangtze River Basin, and also in India, Europe and America. Snake berries are widely used in medicine, such as clearing heat and detoxification, dispersing blood stasis and swelling, cooling blood and hemostasis, treating snakebug bite and so on. In this paper, the chemical constituents and pharmacological effects of Raspberry were summarized. It was found that there were few reports on DPPH radical scavenging activity and tyrosinase inhibitory activity. By means of MCI column chromatography, macroporous resin column chromatography, ODS-A reversed phase chromatography and high performance liquid chromatography, 13 compounds were isolated from Rosaceae plants in Changbai Mountain area. The molecular structures of the six polar compounds were identified as 3-O- 尾-D-glucopyranoxy-6-hydroxy-p-cymene (1), Methyl _ 3N _ 4 (5-trih-ydroxybenzoate) (2). Black Schisandrin (Isolariciresinol-2 偽 -O- 尾 D-xyloside) (6), Apigenin-8-C-glucoside (9), 1- 尾 -D-glucoside [() -1-Hydroxysyringaresinol-1- 尾-D-glucoside] (10), (6RN9R) -3-oxo- 偽 -ionol-尾 -D-glucoside [(6R,) 9R) -3-Oxo- 偽-ionol- 尾-D-glucopyranoside] (13). The DPPH radical scavenging and tyrosinase activity inhibition experiments were carried out on the isolated and identified compounds. The results showed that the DPPH radical scavenging rate and tyrosinase inhibition rate of all compounds were above 90%. The free radical scavenging activity IC50 of compound 10C13 was 2.23 渭 g / mL and 2.07 渭 g / mL, respectively, which was lower than that of positive exhibitors. The inhibitory rate of tyrosinase of compound 2C13 was higher than that of control group, and the inhibitory activity of tyrosinase of compound 13 was 3.3 渭 g / mL.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R284;O652.6
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本文編號(hào)：2310018