【摘要】：目的:利用分光光度法分別測定水果酵素中多種功效酶的活性。方法:借助分光光度計(jì),采用3,5-二硝基水楊酸法、福林法、銅皂法和鄰苯三酚終止法分別對(duì)液狀水果酵素中淀粉酶、蛋白酶、脂肪酶以及超氧化物歧化酶的活力進(jìn)行測定。結(jié)果:液狀水果酵素中淀粉酶活力為1.968 U/mL,蛋白酶活力為5.421 U/mL,脂肪酶活力為1.106 U/mL,超氧化物歧化酶活力為10.758 U/mL。結(jié)論:液狀水果酵素中SOD酶活性最高,蛋白酶次之,淀粉酶和脂肪酶活性較低;后續(xù)實(shí)驗(yàn)中可優(yōu)化發(fā)酵條件來提高其中的淀粉酶與脂肪酶活力。
[Abstract]:Objective: to determine the activities of several effective enzymes in fruit enzymes by spectrophotometry. Methods: the activities of amylase, protease, lipase and superoxide dismutase in liquid fruit ferment were determined by spectrophotometer, 3'5 'dinitrosalicylic acid method, forin method, copper soap method and pyrogallol termination method, respectively. Results: the activity of amylase, protease, lipase, superoxide dismutase and superoxide dismutase were 1.968 U / mL, 5.421 U / mL, 1.106 U / mL and 10.758 U / mL, respectively. Conclusion: the activity of SOD is the highest in the liquid fruit enzyme, followed by protease, and the activity of amylase and lipase is lower, and the fermentation conditions can be optimized to improve the activity of amylase and lipase.
【作者單位】： 河南大學(xué)生命科學(xué)學(xué)院生物工程研究所;
【基金】：教育部高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金項(xiàng)目(20124103120002) 河南省科技攻關(guān)計(jì)劃項(xiàng)目(132102310421) 河南大學(xué)研究生優(yōu)秀學(xué)位論文培育計(jì)劃(理學(xué)碩士)(Y1425010)
【分類號(hào)】：O657.3;TQ925

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