【摘要】：目的:建立貞芪扶正顆粒的高效液相色譜(HPLC)指紋圖譜。方法:采用高效液相色譜-蒸發(fā)光散射檢測法(HPLCELSD)。色譜柱為Agilent Zorbax Eclipse XDB-C_(18),流動(dòng)相為乙腈-水(梯度洗脫),流速為1.0 mL/min,柱溫為40℃,檢測器蒸發(fā)光溫度為50℃,進(jìn)樣量為10μL。以特女貞苷為參照,測定7批貞芪扶正顆粒的HPLC圖譜,采用《中藥色譜指紋圖譜相似度評價(jià)系統(tǒng)》(2004 A)版進(jìn)行共有峰指認(rèn)和相似度評價(jià)。結(jié)果:7批貞芪扶正顆粒的HPLC圖譜有13個(gè)共有峰。經(jīng)驗(yàn)證,7批樣品HPLC圖譜中有3批樣品相似度0.9,與對照指紋圖譜具有較好一致性;4批樣品相似度0.9,與對照指紋圖譜一致性較差。結(jié)論:該研究所建指紋圖譜可為貞芪扶正顆粒的質(zhì)量評價(jià)提供參考。
[Abstract]:Objective: to establish (HPLC) fingerprint of Zhenqi Fuzheng granules by HPLC. Methods: (HPLCELSD). Was detected by high performance liquid chromatography-evaporation light scattering. The chromatographic column is Agilent Zorbax Eclipse XDB-C_ (18), the mobile phase is acetonitrile-water (gradient elution), the flow rate is 1.0 mL/min, and the column temperature is 40 鈩，

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