金銀花與山銀花的HPLC指紋圖譜鑒別研究
發(fā)布時(shí)間：2018-10-15 09:36
【摘要】：目的建立金銀花的HPLC對(duì)照指紋圖譜,并在相同色譜條件下測(cè)定山銀花的HPLC指紋圖譜,通過(guò)計(jì)算相似度來(lái)區(qū)分山銀花與金銀花。方法色譜柱Phenomenex Luna 5μm C18(2)100 A(250 mm×4.6 mm,5μm);柱溫40℃,以乙腈-0.5%磷酸溶液為流動(dòng)相梯度洗脫,體積流量1 m L/min,檢測(cè)波長(zhǎng)為350 nm,相似度計(jì)算采取屏蔽大峰積分的方法。結(jié)果建立了金銀花的HPLC對(duì)照指紋圖譜,12批金銀花相似度均在0.95以上,山銀花相似度均小于0.80。結(jié)論 350nm下金銀花的HPLC指紋圖譜能有效區(qū)分金銀花與山銀花藥材,屏蔽大峰積分的相似度評(píng)價(jià)方法比較準(zhǔn)確地反映了金銀花與山銀花間化學(xué)成分的差異。
[Abstract]:Objective to establish the HPLC fingerprint of honeysuckle and to determine the HPLC fingerprint of Lonicera japonica under the same chromatographic conditions, and to distinguish the honeysuckle from the honeysuckle by calculating the similarity. Methods the column temperature of Phenomenex Luna 5 渭 m C18 (2) 100A (250 mm 脳 4.6 mm,5 渭 m);) was 40 鈩，
本文編號(hào)：2272121
[Abstract]:Objective to establish the HPLC fingerprint of honeysuckle and to determine the HPLC fingerprint of Lonicera japonica under the same chromatographic conditions, and to distinguish the honeysuckle from the honeysuckle by calculating the similarity. Methods the column temperature of Phenomenex Luna 5 渭 m C18 (2) 100A (250 mm 脳 4.6 mm,5 渭 m);) was 40 鈩，
本文編號(hào)：2272121
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