【摘要】：碳納米量子點(diǎn)(carbon quantum dots,CDs)作為新型的納米材料,由于其制備方式簡(jiǎn)單多樣,水溶性和穩(wěn)定性好,并且具有獨(dú)特的光學(xué)特性,因而在生物醫(yī)學(xué)、生物成像等領(lǐng)域得到了廣闊的應(yīng)用。本文采用水熱法制備了三種含氮、氧元素的碳納米量子點(diǎn),利用其熒光特性建立對(duì)D-青霉胺、草甘膦和日落黃的靈敏檢測(cè)。具體研究?jī)?nèi)容如下:1.基于碳點(diǎn)的熒光恢復(fù)檢測(cè)D-青酶胺及邏輯應(yīng)用基于碳點(diǎn)獨(dú)特的光學(xué)性質(zhì),研究并制備了一種新穎、靈敏的碳點(diǎn)熒光探針,成功用于人體血清樣本中D-青酶胺的測(cè)定。將檸檬酸鈉(0.2 g)、碳銨(0.2 g)、二次蒸餾水(10 ml)依次加入到高壓不銹鋼反應(yīng)釜中,在180?C的烘箱中反應(yīng)4小時(shí)后得到淺黃色溶液,溶液透析12小時(shí)后,冷凍干燥得到最終產(chǎn)品。碳點(diǎn)的激發(fā)波長(zhǎng)和發(fā)射波長(zhǎng)分別為358 nm和442 nm。其熒光強(qiáng)度在Hg~(2+)存在時(shí)明顯降低,在加入D-青酶胺后,由于D-青酶胺和Hg~(2+)之間存在較強(qiáng)的親和力,導(dǎo)致碳點(diǎn)的熒光恢復(fù)。鑒于此,我們首次設(shè)計(jì)了“AND”的邏輯門和特定的熒光探針來(lái)檢測(cè)D-青酶胺。測(cè)定D-青酶胺的線性范圍2.0~24.0μmol·L-1,相關(guān)系數(shù)(R2)為0.998,檢出限為0.6μmol·L-1。2.基于碳點(diǎn)的熒光共振能量轉(zhuǎn)移檢測(cè)草甘膦及邏輯應(yīng)用提出了基于碳點(diǎn)和草甘膦之間的熒光共振能量轉(zhuǎn)移的新方法來(lái)檢測(cè)草甘膦。用檸檬酸和Tris-HCl通過(guò)水熱一步合成法制得碳點(diǎn)。碳點(diǎn)的最大激發(fā)波長(zhǎng)為340nm,在410 nm處發(fā)出明亮的藍(lán)色熒光,草甘膦的存在可以有效地猝滅碳點(diǎn)的熒光。我們利用這一現(xiàn)象設(shè)計(jì)了一個(gè)“AND”邏輯門。據(jù)此建立了高選擇性檢測(cè)草甘膦的熒光光度法。在優(yōu)化的條件下,其線性范圍為0.02~2.0μmol·L-1,檢出限為0.6μmol·L-1。該法用于檢測(cè)水樣中草甘膦,結(jié)果滿意。3.基于碳點(diǎn)的熒光共振能量轉(zhuǎn)移作為檢測(cè)日落黃的熒光探針于坩堝中直接將N-羥乙基乙二胺三乙酸加熱得到了水溶性好、粒徑大小統(tǒng)一的碳點(diǎn)。在366 nm處最大激發(fā),在423 nm波長(zhǎng)處發(fā)出藍(lán)色的熒光。由于碳點(diǎn)和日落黃之間發(fā)生熒光共振能量轉(zhuǎn)移,從而導(dǎo)致碳點(diǎn)的熒光顯著猝滅。據(jù)此提出了檢測(cè)日落黃的新方法,測(cè)定濃度范圍從0.3到8.0μmol·L~(-1),檢出限(3σ/k)達(dá)到79.6 nmol·L~(-1)。此方法成功應(yīng)用于檢測(cè)飲料中的日落黃含量。
[Abstract]:As a new type of nano-material, carbon nanodots (carbon quantum dots,CDs) have been widely used in biomedical, biomedical and imaging fields because of their simple preparation methods, good water solubility, good stability and unique optical properties. In this paper, three kinds of carbon nanoscale quantum dots containing nitrogen and oxygen were prepared by hydrothermal method. The sensitive detection of D- penicillamine, glyphosate and sunset yellow was established by using their fluorescence characteristics. The specific contents of the study are as follows: 1. Based on the unique optical properties of carbon spots, a novel and sensitive carbon point fluorescence probe was developed, which was successfully used for the determination of D- cyanoamine in human serum samples. Sodium citrate (0.2 g), ammonium carbonate (0.2 g), secondary distilled water (10 ml) was added to the autoclave in turn. The light yellow solution was obtained after 4 hours reaction in the oven of C, 12 hours after dialysis, the final product was obtained by freeze-drying. The excitation and emission wavelengths of carbon spots are 358 nm and 442 nm., respectively. The fluorescence intensity decreased obviously when Hg~ (2) was present. After the addition of D- cyanoamine, the fluorescence of carbon point recovered due to the strong affinity between D- cyanoamine and Hg~ (2). In view of this, we first designed a "AND" logic gate and a specific fluorescent probe to detect D-cyanoamine. The linear range for the determination of D-cyanide was 2.0 渭 mol / L ~ (-1), the correlation coefficient (R2) was 0.998, and the detection limit was 0.6 渭 mol L ~ (-1). Detection of glyphosate based on carbon Point fluorescence Resonance Energy transfer and its logical applications A new method based on fluorescence resonance energy transfer between carbon point and glyphosate is proposed to detect glyphosate. Carbon dots were prepared by hydrothermal synthesis with citric acid and Tris-HCl. The maximum excitation wavelength of the carbon point is 340 nm, and bright blue fluorescence is emitted at 410 nm. The existence of glyphosate can effectively quench the fluorescence of the carbon point. We use this phenomenon to design a "AND" logic gate. A high selective fluorescence spectrophotometric method for the determination of glyphosate was established. Under the optimized conditions, the linear range is 0.02 渭 mol L -1 and the detection limit is 0.6 渭 mol L -1. The method has been applied to the determination of glyphosate in water with satisfactory results. Fluorescence resonance energy transfer based on carbon point was used as a fluorescence probe for detecting sunset yellow in crucible to directly heat N- hydroxyethyl ethylenediamine triacetic acid to obtain a carbon point with good water solubility and uniform particle size. The maximum excitation is at 366 nm, and the blue fluorescence is emitted at 423 nm wavelength. Fluorescence resonance energy transfer occurs between carbon point and sunset yellow, which leads to fluorescence quenching of carbon point. A new method for the detection of sunset yellow is proposed. The concentration range is from 0.3 to 8.0 渭 mol L ~ (-1), and the detection limit (3 蟽 / k) is 79.6 nmol L ~ (-1). This method has been successfully applied to the determination of sunset yellow in beverage.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3;TB383.1

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