【摘要】：碳納米量子點(carbon quantum dots,CDs)作為新型的納米材料,由于其制備方式簡單多樣,水溶性和穩(wěn)定性好,并且具有獨特的光學特性,因而在生物醫(yī)學、生物成像等領(lǐng)域得到了廣闊的應用。本文采用水熱法制備了三種含氮、氧元素的碳納米量子點,利用其熒光特性建立對D-青霉胺、草甘膦和日落黃的靈敏檢測。具體研究內(nèi)容如下:1.基于碳點的熒光恢復檢測D-青酶胺及邏輯應用基于碳點獨特的光學性質(zhì),研究并制備了一種新穎、靈敏的碳點熒光探針,成功用于人體血清樣本中D-青酶胺的測定。將檸檬酸鈉(0.2 g)、碳銨(0.2 g)、二次蒸餾水(10 ml)依次加入到高壓不銹鋼反應釜中,在180?C的烘箱中反應4小時后得到淺黃色溶液,溶液透析12小時后,冷凍干燥得到最終產(chǎn)品。碳點的激發(fā)波長和發(fā)射波長分別為358 nm和442 nm。其熒光強度在Hg~(2+)存在時明顯降低,在加入D-青酶胺后,由于D-青酶胺和Hg~(2+)之間存在較強的親和力,導致碳點的熒光恢復。鑒于此,我們首次設計了“AND”的邏輯門和特定的熒光探針來檢測D-青酶胺。測定D-青酶胺的線性范圍2.0~24.0μmol·L-1,相關(guān)系數(shù)(R2)為0.998,檢出限為0.6μmol·L-1。2.基于碳點的熒光共振能量轉(zhuǎn)移檢測草甘膦及邏輯應用提出了基于碳點和草甘膦之間的熒光共振能量轉(zhuǎn)移的新方法來檢測草甘膦。用檸檬酸和Tris-HCl通過水熱一步合成法制得碳點。碳點的最大激發(fā)波長為340nm,在410 nm處發(fā)出明亮的藍色熒光,草甘膦的存在可以有效地猝滅碳點的熒光。我們利用這一現(xiàn)象設計了一個“AND”邏輯門。據(jù)此建立了高選擇性檢測草甘膦的熒光光度法。在優(yōu)化的條件下,其線性范圍為0.02~2.0μmol·L-1,檢出限為0.6μmol·L-1。該法用于檢測水樣中草甘膦,結(jié)果滿意。3.基于碳點的熒光共振能量轉(zhuǎn)移作為檢測日落黃的熒光探針于坩堝中直接將N-羥乙基乙二胺三乙酸加熱得到了水溶性好、粒徑大小統(tǒng)一的碳點。在366 nm處最大激發(fā),在423 nm波長處發(fā)出藍色的熒光。由于碳點和日落黃之間發(fā)生熒光共振能量轉(zhuǎn)移,從而導致碳點的熒光顯著猝滅。據(jù)此提出了檢測日落黃的新方法,測定濃度范圍從0.3到8.0μmol·L~(-1),檢出限(3σ/k)達到79.6 nmol·L~(-1)。此方法成功應用于檢測飲料中的日落黃含量。
[Abstract]:As a new type of nano-material, carbon nanodots (carbon quantum dots,CDs) have been widely used in biomedical, biomedical and imaging fields because of their simple preparation methods, good water solubility, good stability and unique optical properties. In this paper, three kinds of carbon nanoscale quantum dots containing nitrogen and oxygen were prepared by hydrothermal method. The sensitive detection of D- penicillamine, glyphosate and sunset yellow was established by using their fluorescence characteristics. The specific contents of the study are as follows: 1. Based on the unique optical properties of carbon spots, a novel and sensitive carbon point fluorescence probe was developed, which was successfully used for the determination of D- cyanoamine in human serum samples. Sodium citrate (0.2 g), ammonium carbonate (0.2 g), secondary distilled water (10 ml) was added to the autoclave in turn. The light yellow solution was obtained after 4 hours reaction in the oven of C, 12 hours after dialysis, the final product was obtained by freeze-drying. The excitation and emission wavelengths of carbon spots are 358 nm and 442 nm., respectively. The fluorescence intensity decreased obviously when Hg~ (2) was present. After the addition of D- cyanoamine, the fluorescence of carbon point recovered due to the strong affinity between D- cyanoamine and Hg~ (2). In view of this, we first designed a "AND" logic gate and a specific fluorescent probe to detect D-cyanoamine. The linear range for the determination of D-cyanide was 2.0 渭 mol / L ~ (-1), the correlation coefficient (R2) was 0.998, and the detection limit was 0.6 渭 mol L ~ (-1). Detection of glyphosate based on carbon Point fluorescence Resonance Energy transfer and its logical applications A new method based on fluorescence resonance energy transfer between carbon point and glyphosate is proposed to detect glyphosate. Carbon dots were prepared by hydrothermal synthesis with citric acid and Tris-HCl. The maximum excitation wavelength of the carbon point is 340 nm, and bright blue fluorescence is emitted at 410 nm. The existence of glyphosate can effectively quench the fluorescence of the carbon point. We use this phenomenon to design a "AND" logic gate. A high selective fluorescence spectrophotometric method for the determination of glyphosate was established. Under the optimized conditions, the linear range is 0.02 渭 mol L -1 and the detection limit is 0.6 渭 mol L -1. The method has been applied to the determination of glyphosate in water with satisfactory results. Fluorescence resonance energy transfer based on carbon point was used as a fluorescence probe for detecting sunset yellow in crucible to directly heat N- hydroxyethyl ethylenediamine triacetic acid to obtain a carbon point with good water solubility and uniform particle size. The maximum excitation is at 366 nm, and the blue fluorescence is emitted at 423 nm wavelength. Fluorescence resonance energy transfer occurs between carbon point and sunset yellow, which leads to fluorescence quenching of carbon point. A new method for the detection of sunset yellow is proposed. The concentration range is from 0.3 to 8.0 渭 mol L ~ (-1), and the detection limit (3 蟽 / k) is 79.6 nmol L ~ (-1). This method has been successfully applied to the determination of sunset yellow in beverage.
【學位授予單位】：西南大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3;TB383.1

【相似文獻】

相關(guān)期刊論文 前10條

1 王成;程軍喜;張小軍;;飲料中日落黃和胭脂紅含量同時測定的分光光度法[J];職業(yè)與健康;2008年15期

2 張永生;魏新軍;南海娟;顏振敏;;支持向量回歸分光光度法同時測定飲料中檸檬黃與日落黃[J];食品研究與開發(fā);2009年08期

3 梁敏思;鄒永德;王玉東;白燕;;玻碳電極測定飲料中檸檬黃、日落黃[J];現(xiàn)代預防醫(yī)學;2009年18期

4 林壯森;張美珍;江英志;蔡澤鳳;陳楚珊;;分光光度法快速檢測飲料中日落黃的研究[J];廣州化工;2011年07期

5 李積慧;杜國榮;康俊;蔡文生;邵學廣;;陰離子交換樹脂結(jié)合近紅外漫反射光譜測定碳酸飲料中日落黃[J];分析化學;2011年06期

6 孫婭娜;朱蕾;崔芳;;高效液相色譜法測定糕點中的檸檬黃、日落黃和亮藍[J];食品科技;2011年12期

7 赫春香;楊晶;;日落黃分子印跡電化學傳感器的研制與應用[J];遼寧師范大學學報(自然科學版);2012年01期

8 初紅濤;張暉;王軍;靳鳳龍;蘇立強;;谷物中檸檬黃和日落黃高效液相色譜檢測方法的優(yōu)化[J];安徽農(nóng)業(yè)科學;2012年09期

9 渠志華;張明月;黨亞敏;李建平;馬永民;左桐;;非發(fā)酵性豆制品中檸檬黃、胭脂紅和日落黃的測定[J];中國衛(wèi)生檢驗雜志;2012年07期

10 劉宇;謝志海;蔡清;降曉艷;;熒光光譜法測定飲料中的日落黃[J];分析科學學報;2012年04期

相關(guān)會議論文 前3條

1 繆雪艷;王瑋;熊博;周曉東;胡繼明;;熱透鏡和回射干涉聯(lián)用同時無分離檢測蔗糖和日落黃[A];中國化學會第十屆全國發(fā)光分析學術(shù)研討會論文集[C];2011年

2 羅培麗;劉潔;冶保獻;;聚L-半胱氨酸修飾玻碳電極用于飲料中日落黃的檢測[A];河南省化學會2012年學術(shù)年會論文摘要集[C];2012年

3 張永生;魏新軍;顏振敏;南海娟;;多元線性回歸分光光度法同時測定飲料中三種色素[A];第十屆中國科協(xié)年會論文集（三）[C];2008年

相關(guān)重要報紙文章 前5條

1 曉康;解讀“日落黃”[N];醫(yī)藥養(yǎng)生保健報;2005年

2 張儉波;解讀日落黃[N];健康報;2005年

3 京蘭;檸檬黃與檸檬酸并非“兄弟”[N];消費日報;2006年

4 程二萍 記者 戴正聰;綠盛牛肉干被查含日落黃北京遭下架[N];中國食品質(zhì)量報;2005年

5 記者　 吳穎;北京首發(fā)食品預警：慎食“艷麗”食品[N];北京現(xiàn)代商報;2006年

相關(guān)碩士學位論文 前4條

1 楊晶;日落黃分子印跡修飾電極的制備及其應用研究[D];遼寧師范大學;2012年

2 邢s，

本文編號：2253194