【摘要】：基于表面增強(qiáng)拉曼光譜技術(shù)(surface enhanced Raman spectroscopy,SERS),研制出對(duì)食品中烏洛托品進(jìn)行現(xiàn)場(chǎng)快速分析檢測(cè)的系統(tǒng)。檢測(cè)系統(tǒng)由多功能前處理模塊、拉曼光學(xué)模塊、嵌入式主控模塊三部分組成,實(shí)現(xiàn)了待測(cè)樣品的前處理,拉曼光譜數(shù)據(jù)的產(chǎn)生、收集、傳輸與處理。其中,多功能前處理模塊將化學(xué)實(shí)驗(yàn)中經(jīng)常用到的離心、超聲、揮發(fā)三種功能集于一體,精簡(jiǎn)了前處理實(shí)驗(yàn)中需要用到的設(shè)備;拉曼光學(xué)模塊采用探頭光路與分光系統(tǒng)合為一體的整體式設(shè)計(jì),并在探頭光路中使用數(shù)值孔徑為0.6的非球面透鏡,同時(shí)利用交叉非對(duì)稱Czerny-Turner結(jié)構(gòu)對(duì)分光系統(tǒng)進(jìn)行了設(shè)計(jì);嵌入式主控模塊采用自主研發(fā)的FPGA+ARM雙核心主控電路,配合CCD的制冷電路與驅(qū)動(dòng)電路,搭載μC/OS-Ⅲ操作系統(tǒng),實(shí)現(xiàn)了對(duì)CCD上拉曼光譜數(shù)據(jù)的采集、傳輸與處理。利用檸檬酸鈉制備納米金(liquid colloidal gold nanoparticle,LCP-1)作為增強(qiáng)試劑對(duì)烏洛托品進(jìn)行檢測(cè),將烏洛托品的拉曼特征峰選取在1 047cm~(-1)處,對(duì)烏洛托品標(biāo)準(zhǔn)品和含有烏洛托品的腐竹與米粉樣品進(jìn)行檢測(cè)實(shí)驗(yàn),實(shí)驗(yàn)結(jié)果表明所設(shè)計(jì)的檢測(cè)系統(tǒng)可以實(shí)現(xiàn)對(duì)烏洛托品的準(zhǔn)確識(shí)別,對(duì)烏洛托品標(biāo)準(zhǔn)品的檢測(cè)限可達(dá)0.01 mg·L~(-1),對(duì)腐竹和米粉樣品中烏洛托品的檢出限可達(dá)0.5 mg·L~(-1),檢測(cè)時(shí)間小于20min,實(shí)現(xiàn)了食品中烏洛托品的現(xiàn)場(chǎng)快速檢測(cè)。
[Abstract]:Based on surface-enhanced Raman spectroscopy (surface enhanced Raman spectroscopy,SERS), a system for rapid analysis and detection of urotonin in food was developed. The detection system consists of three parts: multifunctional pre-processing module, Raman optical module and embedded main control module. It realizes the pre-processing of samples to be tested, the generation, collection, transmission and processing of Raman spectrum data. The multifunctional preprocessing module integrates the three functions of centrifuge, ultrasonic and volatilization, which are often used in chemical experiment, and simplifies the equipment that needs to be used in the pretreatment experiment. The Raman optical module is designed by the combination of the probe optical path and the splitter system. The aspheric lens with a numerical aperture of 0.6 is used in the probe optical path. At the same time, a cross-asymmetric Czerny-Turner structure is used to design the optical separation system. The embedded main control module adopts the FPGA ARM dual core master control circuit which is developed by ourselves. With the refrigeration circuit and driving circuit of CCD, the embedded main control module is equipped with 渭 C / OS- 鈪，

本文編號(hào)：2214113