基于SERS的食品中烏洛托品快速檢測系統(tǒng)設(shè)計
發(fā)布時間：2018-08-30 19:51
【摘要】：基于表面增強拉曼光譜技術(shù)(surface enhanced Raman spectroscopy,SERS),研制出對食品中烏洛托品進行現(xiàn)場快速分析檢測的系統(tǒng)。檢測系統(tǒng)由多功能前處理模塊、拉曼光學(xué)模塊、嵌入式主控模塊三部分組成,實現(xiàn)了待測樣品的前處理,拉曼光譜數(shù)據(jù)的產(chǎn)生、收集、傳輸與處理。其中,多功能前處理模塊將化學(xué)實驗中經(jīng)常用到的離心、超聲、揮發(fā)三種功能集于一體,精簡了前處理實驗中需要用到的設(shè)備;拉曼光學(xué)模塊采用探頭光路與分光系統(tǒng)合為一體的整體式設(shè)計,并在探頭光路中使用數(shù)值孔徑為0.6的非球面透鏡,同時利用交叉非對稱Czerny-Turner結(jié)構(gòu)對分光系統(tǒng)進行了設(shè)計;嵌入式主控模塊采用自主研發(fā)的FPGA+ARM雙核心主控電路,配合CCD的制冷電路與驅(qū)動電路,搭載μC/OS-Ⅲ操作系統(tǒng),實現(xiàn)了對CCD上拉曼光譜數(shù)據(jù)的采集、傳輸與處理。利用檸檬酸鈉制備納米金(liquid colloidal gold nanoparticle,LCP-1)作為增強試劑對烏洛托品進行檢測,將烏洛托品的拉曼特征峰選取在1 047cm~(-1)處,對烏洛托品標準品和含有烏洛托品的腐竹與米粉樣品進行檢測實驗,實驗結(jié)果表明所設(shè)計的檢測系統(tǒng)可以實現(xiàn)對烏洛托品的準確識別,對烏洛托品標準品的檢測限可達0.01 mg·L~(-1),對腐竹和米粉樣品中烏洛托品的檢出限可達0.5 mg·L~(-1),檢測時間小于20min,實現(xiàn)了食品中烏洛托品的現(xiàn)場快速檢測。
[Abstract]:Based on surface-enhanced Raman spectroscopy (surface enhanced Raman spectroscopy,SERS), a system for rapid analysis and detection of urotonin in food was developed. The detection system consists of three parts: multifunctional pre-processing module, Raman optical module and embedded main control module. It realizes the pre-processing of samples to be tested, the generation, collection, transmission and processing of Raman spectrum data. The multifunctional preprocessing module integrates the three functions of centrifuge, ultrasonic and volatilization, which are often used in chemical experiment, and simplifies the equipment that needs to be used in the pretreatment experiment. The Raman optical module is designed by the combination of the probe optical path and the splitter system. The aspheric lens with a numerical aperture of 0.6 is used in the probe optical path. At the same time, a cross-asymmetric Czerny-Turner structure is used to design the optical separation system. The embedded main control module adopts the FPGA ARM dual core master control circuit which is developed by ourselves. With the refrigeration circuit and driving circuit of CCD, the embedded main control module is equipped with 渭 C / OS- 鈪，
本文編號：2214113
[Abstract]:Based on surface-enhanced Raman spectroscopy (surface enhanced Raman spectroscopy,SERS), a system for rapid analysis and detection of urotonin in food was developed. The detection system consists of three parts: multifunctional pre-processing module, Raman optical module and embedded main control module. It realizes the pre-processing of samples to be tested, the generation, collection, transmission and processing of Raman spectrum data. The multifunctional preprocessing module integrates the three functions of centrifuge, ultrasonic and volatilization, which are often used in chemical experiment, and simplifies the equipment that needs to be used in the pretreatment experiment. The Raman optical module is designed by the combination of the probe optical path and the splitter system. The aspheric lens with a numerical aperture of 0.6 is used in the probe optical path. At the same time, a cross-asymmetric Czerny-Turner structure is used to design the optical separation system. The embedded main control module adopts the FPGA ARM dual core master control circuit which is developed by ourselves. With the refrigeration circuit and driving circuit of CCD, the embedded main control module is equipped with 渭 C / OS- 鈪，
本文編號：2214113
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