【摘要】：建立超聲輔助溶劑棒微萃取-高效液相色譜(UA-SBME-HPLC)方法,用于快速測(cè)定嬰兒配方奶粉中的雙酚A(BPA)。設(shè)計(jì)超聲輔助溶劑棒微萃取系統(tǒng),優(yōu)化后的最佳萃取條件為:溶劑棒的有效長(zhǎng)度為30 mm,萃取溶劑為正辛醇,攪拌速度為600 r/min,超聲時(shí)間10 min,萃取時(shí)間15 min,pH=6的HCl溶液為給體相,0.12 mol/L NaOH溶液為受體相,NaCl的加入濃度為60 g/L。在最優(yōu)萃取條件下,該方法對(duì)BPA的平均富集倍數(shù)為102倍,方法的線性范圍為0.1~100μg/L(r=0.9989),檢出限為0.35μg/L(S/N=3),相對(duì)標(biāo)準(zhǔn)偏差RSD小于4.32%(n=6),加標(biāo)回收率為89.7%~102.8%。研究表明該方法綠色環(huán)保,可用于準(zhǔn)確快速測(cè)定嬰兒配方奶粉中的雙酚A。
[Abstract]:An ultrasonic assisted solvent rod microextraction (UA-SBME-HPLC) method was developed for the rapid determination of bisphenol A (BPA). In infant formula. The ultrasonic assisted solvent rod microextraction system was designed. The optimum extraction conditions were as follows: the effective length of the solvent rod was 30 mm, and the solvent was n-octanol. The concentration of HCl solution with stirring speed of 600r / min, ultrasonic time of 10 min, extraction time of 15 min,pH=6 and donor phase of 0.12 mol/L NaOH solution was 60 g / L. Under the optimum extraction conditions, the average enrichment multiple of BPA was 102.The linear range of the method was 0.1 渭 g / L (r ~ (0.9989), the detection limit was 0.35 渭 g / L (S/N=3), the relative standard deviation (RSD) was less than 4.32% (n ~ (6), and the recovery rate was 89.72.8%. The results show that the method is green and can be used for the accurate and rapid determination of bisphenol A in infant formula.
【作者單位】： 三峽大學(xué)生物與制藥學(xué)院;
【基金】：湖北省教育廳科研項(xiàng)目(B2015256) 宜昌市科技局自然基礎(chǔ)科學(xué)研究與應(yīng)用專項(xiàng)(A15-302-a05) 三峽大學(xué)科學(xué)基金項(xiàng)目(KJ2013B070)
【分類號(hào)】：O657.72;TS252.51

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 王蕾;李曉東;;高效液相色譜法檢測(cè)嬰兒配方奶粉中唾液酸[J];中國(guó)乳品工業(yè);2013年05期

2 莫文玲;趙雅萍;柳華杰;郭虎森;劉明光;;表面活性劑輔助溶劑熱方法合成PbS納米立方微晶的研究(英文)[J];南開(kāi)大學(xué)學(xué)報(bào)(自然科學(xué)版);2005年05期

，

本文編號(hào)：2212739