利用高效液相色譜-串聯(lián)質(zhì)譜追蹤酸奶發(fā)酵過(guò)程中的酪蛋白磷酸肽
發(fā)布時(shí)間：2018-08-25 08:05
【摘要】：酪蛋白磷酸肽(CPPs)是酪蛋白被磷酸化的片段,具有促進(jìn)礦物元素吸收、抗氧化、預(yù)防齲齒等多種生物功能。該文利用高效液相色譜-串聯(lián)質(zhì)譜研究牛奶經(jīng)嗜熱鏈球菌(Streptococcus thermophilus)和保加利亞乳桿菌(Lactobacillus debrueckii subsp.bulgaricus)發(fā)酵后CPPs的釋放規(guī)律。結(jié)果表明,在內(nèi)源性蛋白酶的作用下牛奶含內(nèi)源性CPPs,內(nèi)源性CPPs主要來(lái)源于高豐度酪蛋白αs1-CN和β-CN。經(jīng)乳酸菌發(fā)酵后,在乳酸菌蛋白酶的作用下更多酪蛋白的磷酸化位點(diǎn)暴露而產(chǎn)生大量CPPs,其中含SpSpSpEE特征結(jié)構(gòu)的CPPs也在酸奶中檢測(cè)到,CPPs的釋放與酪蛋白結(jié)構(gòu)密切相關(guān)。研究結(jié)果表明,牛奶經(jīng)乳酸菌發(fā)酵后釋放大量含特征結(jié)構(gòu)SpSpSpEE的CPPs,可提高結(jié)合礦物元素的能力,從而增強(qiáng)其促進(jìn)人體健康的生物功能。
[Abstract]:Casein phosphopeptides (CPPs) are phosphorylated fragments of casein, which have many biological functions, such as promoting mineral absorption, antioxidant activity and preventing dental caries. In this paper, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) was used to study milk transdermal Streptococcus thermophilus (Streptococcus thermophilus) and Lactobacillus debrueckii subsp. bulgaricu. The results showed that the milk contained endogenous CPPs under the action of endogenous protease, and the endogenous CPPs mainly came from high-abundance casein alphas1-CN and beta-CN. After fermentation by lactobacillus, more phosphorylation sites of casein were exposed under the action of Lactobacillus proteinase and a large number of CPPs were produced, including SpSpSpSpSpSpEE characteristics. Structural CPPs were also detected in yoghurt, and the release of CPPs was closely related to casein structure. The results showed that large amounts of CPPs containing characteristic structure SpSpSpEE were released after fermentation by lactic acid bacteria, which could improve the ability of binding mineral elements and enhance the biological function of promoting human health.
【作者單位】： 中國(guó)科學(xué)院大連化學(xué)物理研究所中國(guó)科學(xué)院分離分析化學(xué)重點(diǎn)實(shí)驗(yàn)室國(guó)家色譜研究分析中心;中國(guó)科學(xué)院大學(xué);
【基金】：中國(guó)科學(xué)院分離分析化學(xué)重點(diǎn)實(shí)驗(yàn)室開放基金(KL-1602)~~
【分類號(hào)】：O657.63;TS252.54
本文編號(hào)：2202278
[Abstract]:Casein phosphopeptides (CPPs) are phosphorylated fragments of casein, which have many biological functions, such as promoting mineral absorption, antioxidant activity and preventing dental caries. In this paper, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) was used to study milk transdermal Streptococcus thermophilus (Streptococcus thermophilus) and Lactobacillus debrueckii subsp. bulgaricu. The results showed that the milk contained endogenous CPPs under the action of endogenous protease, and the endogenous CPPs mainly came from high-abundance casein alphas1-CN and beta-CN. After fermentation by lactobacillus, more phosphorylation sites of casein were exposed under the action of Lactobacillus proteinase and a large number of CPPs were produced, including SpSpSpSpSpSpEE characteristics. Structural CPPs were also detected in yoghurt, and the release of CPPs was closely related to casein structure. The results showed that large amounts of CPPs containing characteristic structure SpSpSpEE were released after fermentation by lactic acid bacteria, which could improve the ability of binding mineral elements and enhance the biological function of promoting human health.
【作者單位】： 中國(guó)科學(xué)院大連化學(xué)物理研究所中國(guó)科學(xué)院分離分析化學(xué)重點(diǎn)實(shí)驗(yàn)室國(guó)家色譜研究分析中心;中國(guó)科學(xué)院大學(xué);
【基金】：中國(guó)科學(xué)院分離分析化學(xué)重點(diǎn)實(shí)驗(yàn)室開放基金(KL-1602)~~
【分類號(hào)】：O657.63;TS252.54
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