【摘要】：目的建立超高效液相色譜-高分辨質(zhì)譜法(UPLC-FT/MS)同時(shí)測(cè)定類葉牡丹Cauorlhyllum robustum中9種三萜皂苷類成分的方法。方法采用正離子模式;色譜柱:ACE Excel 3 C_(18)(100 mm×2.1 mm,3.0μm);保護(hù)柱:ACE Excel 3C_(18)(12.5 mm×4.6 mm,3.0μm);流動(dòng)相為0.1%甲酸水(A)-乙腈(B)溶液,梯度洗脫,體積流量0.40 m L/min,柱溫35℃。結(jié)果定量分析的類葉牡丹中三萜皂苷類成分在考察的濃度范圍內(nèi)呈良好的線性關(guān)系(r0.996);加樣回收率和RSD分別在98.49%~107.03%和1.00%~3.33%。結(jié)論本研究建立的同時(shí)測(cè)定類葉牡丹中9種三萜皂苷類成分的UPLC-FT/MS定量分析方法靈敏度高、分辨率高、質(zhì)量穩(wěn)定并且簡(jiǎn)便、準(zhǔn)確,可為綜合評(píng)價(jià)類葉牡丹的質(zhì)量提供參考。
[Abstract]:Objective to establish a high performance liquid chromatography-high resolution mass spectrometry (UPLC-FT/MS) method for the simultaneous determination of 9 triterpenoid saponins in Cauorlhyllum robustum. Methods Excel 3C18 (100mm 脳 2.1mm) Excel 3C18 (12.5mm 脳 4.6mm) 3.0 渭 m); mobile phase was gradient eluted with 0.1% formic acid water (A) acetonitrile (B) solution, volume flow rate was 0.40 mL / min, column temperature was 35 鈩，

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