【摘要】：目的:建立同時測定益腎衍嗣丸中阿魏酸、梓醇、大黃素和大黃素甲醚含量的方法。方法:采用雙波長高效液相色譜法。色譜柱為Kromasil-C_(18),流動相為甲醇-0.1%磷酸溶液(78∶22,V/V),流速為1.0 mL/min,檢測波長為280 nm(阿魏酸、大黃素和大黃素甲醚)、210 nm(梓醇),柱溫為30℃,進(jìn)樣量為10μL。結(jié)果:阿魏酸、梓醇、大黃素和大黃素甲醚檢測質(zhì)量濃度線性范圍分別為0.090 8~2.270 0μg/mL(r=0.999 9)、0.199 0~4.975 0μg/mL(r=0.999 3)、0.354 0~8.850 0μg/mL(r=0.999 9)、0.3360~8.400 0μg/mL(r=0.999 7);定量限分別為14.66、8.75、10.26、13.68 ng,檢測限分別為5.66、2.67、3.78、4.25 ng;精密度、穩(wěn)定性、重復(fù)性試驗的RSD2.0%;加樣回收率分別為101.15%~102.73%(RSD=0.58%,n=6)、99.20%~101.69%(RSD=1.20%,n=6)、100.90%~101.36%(RSD=0.16%,n=6)、95.64%~98.96%(RSD=1.49%,n=6)。結(jié)論:該方法準(zhǔn)確可靠,操作簡單,適用于益腎衍嗣丸中4種成分含量的同時測定。
[Abstract]:Objective: to establish a method for simultaneous determination of ferulic acid, catalpol, emodin and emodin methyl ether in Yishen Yansi pills. Methods: dual-wavelength high-performance liquid chromatography was used. The chromatographic column was Kromasil-C18, the mobile phase was methanol-0.1% phosphoric acid solution (78: 22V / V), the flow rate was 1.0 mL / min, the detection wavelength was 280 nm (ferulic acid, emodin and emodin methyl ether) 210nm (catalpol), the column temperature was 30 鈩，

本文編號：2164797