【摘要】：基于葡萄糖電化學(xué)氧化原理的葡萄糖傳感器可以直接以電信號(hào)監(jiān)測(cè)葡萄糖濃度,能夠輔助實(shí)現(xiàn)糖尿病診斷的快速篩查、腹膜透析治療的在線監(jiān)測(cè)等,具有重要臨床應(yīng)用前景。相對(duì)于葡萄糖氧化酶型傳感器而言,無(wú)酶電化學(xué)傳感器具有重復(fù)性好、靈敏度高的優(yōu)點(diǎn),但選擇性低、抗干擾性差是該類傳感器需要解決的主要問(wèn)題。電化學(xué)氧化法檢測(cè)血清中葡萄糖的干擾物質(zhì)主要是抗壞血酸和尿素等還原性物質(zhì),本論文在采用壓片法制備的NiOOH/Ni(OH)_2@C電極研究了葡萄糖檢測(cè)靈敏度和選擇性的影響因素及規(guī)律性基礎(chǔ)上,基于抗壞血酸和尿素的電極反應(yīng)過(guò)程為擴(kuò)散控制而葡萄糖為動(dòng)力學(xué)控制的差異性,提出增大電極比表面積以提高檢測(cè)靈敏度和選擇性的研究思路,利用復(fù)合鍍法制備了具有核殼結(jié)構(gòu)的NiOOH/Ni(OH)_2@C電極用以檢測(cè)血清中的葡萄糖。具體內(nèi)容如下：首先,利用陽(yáng)離子型表面活性劑輔助實(shí)現(xiàn)Ni單質(zhì)和碳粉的復(fù)合電沉積獲得具有核殼結(jié)構(gòu)的Ni@C鍍層電極：其次,在堿性介質(zhì)中借助循環(huán)伏安法電化學(xué)氧化鍍層中Ni為Ni(OH)_2;最后,在恒電位條件下形成NiOOH用以檢測(cè)葡萄糖。研究表明,利用NiOOH/Ni(OH)_2@C檢測(cè)葡萄糖時(shí)檢測(cè)線性范圍為5×10~(-5)～1×10~(-2)mol·L~(-1),線性相關(guān)系數(shù)R~2=0.99995,靈敏度為11.84mA·cm-2·mM~(-1),檢測(cè)限為10-6mol·L~(-1)(D=3S/N);檢測(cè)體系的平行性、重復(fù)性以及準(zhǔn)確度均在中國(guó)質(zhì)檢總局發(fā)布的關(guān)于血糖儀通用技術(shù)標(biāo)準(zhǔn)GB/T 19634-2005規(guī)定的范圍之內(nèi)；抗干擾性檢測(cè)結(jié)果表明在檢測(cè)血清中葡萄糖含量時(shí)NiOOH/Ni(OH)_2@C電極抵抗尿素等物質(zhì)的能力優(yōu)于NiOOH/Ni(OH)_2@C電極。
[Abstract]:The glucose sensor based on the principle of electrochemical oxidation of glucose can directly monitor glucose concentration with electrical signals, which can assist in the rapid screening of diabetes diagnosis and on-line monitoring of peritoneal dialysis therapy, etc., which has an important clinical application prospect. Compared with glucose oxidase sensors, enzymatic electrochemical sensors have the advantages of good repeatability and high sensitivity, but low selectivity and poor anti-interference are the main problems that need to be solved. The main interfering substances of glucose in serum are ascorbic acid and urea by electrochemical oxidation method. In this paper, we studied the influencing factors and regularity of glucose detection sensitivity and selectivity at the NiOOH/Ni (OH) _ 2DS _ C electrode prepared by the method of pressing tablets. Based on the difference that the electrode reaction process of ascorbic acid and urea is controlled by diffusion and glucose is controlled by kinetics, the idea of increasing the specific surface area of the electrode to improve the detection sensitivity and selectivity is proposed. A core-shell structure NiOOH/Ni (OH) CO2 @ C electrode was prepared by composite plating for the determination of glucose in serum. The main contents are as follows: firstly, the Ni@C coating electrode with core-shell structure was obtained by using cationic surfactant assisted electrodeposition of Ni and carbon powder. In alkaline medium, Ni is Ni (OH) _ 2 in electrochemical oxidation coating by cyclic voltammetry. Finally, NiOOH is formed at constant potential to detect glucose. The results showed that the linear range of detection for glucose was 5 脳 10 ~ (-5) mol L ~ (-1), the linear correlation coefficient was 0.99995, the sensitivity was 11.84mA cm-2 mm ~ (-1) and the detection limit was 10-6mol L ~ (-1) (D=3S/N). Reproducibility and accuracy are within the scope of GB/T 19634-2005, a general technical standard for blood glucose analyzer issued by the General Administration of quality Supervision, Inspection and Inspection of China; The results of anti-interference test showed that the NiOOH/Ni (OH) _ 2@ C electrode could resist urea and other substances better than the NiOOH/Ni (OH) _ s _ 2r _ 2C electrode when the glucose content in the serum was detected.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R446.1;O657.1

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