【摘要】：金、銀納米粒子具有獨特的光學(xué)、電學(xué)和化學(xué)性質(zhì),在材料、信息及生命科學(xué)等領(lǐng)域顯示出廣闊的應(yīng)用前景。因此,金、銀納米粒子及其復(fù)合材料的制備與應(yīng)用已經(jīng)成為了當(dāng)今科學(xué)研究的熱點領(lǐng)域之一。本文合成了水溶性共軛聚電解質(zhì)(P1、PPESO3、P2)穩(wěn)定的貴金屬納米復(fù)合材料,用于構(gòu)建三聚氰胺、生物硫醇及依替米星靈敏檢測的傳感體系。具體內(nèi)容包括以下三個部分:(1)設(shè)計合成了一種新型納米比率探針P1-AgNPs,實現(xiàn)了三聚氰胺高靈敏、高選擇性的熒光及比色檢測。含吡啶基團(tuán)的P1不僅是優(yōu)良的雙發(fā)射熒光探針,還起到穩(wěn)定銀納米顆粒的作用。隨著三聚氰胺的加入,由于三聚氰胺的氨基與銀納米優(yōu)異的結(jié)合能力,形成了三聚氰胺覆蓋的P1-AgNPs,使得P1在386 nm處的熒光部分恢復(fù),與此同時由于三聚氰胺誘導(dǎo)的團(tuán)聚,導(dǎo)致P1在488 nm處的熒光進(jìn)一步猝滅。因而可以利用比率熒光檢測三聚氰胺。另外,三聚氰胺誘導(dǎo)P1-AgNPs的團(tuán)聚現(xiàn)象可以用于比率比色法檢測三聚氰胺。熒光法和比色法檢測三聚氰胺的檢出限低至0.1和0.45 nM。(2)以熒光共軛聚電解質(zhì)PPESO3為穩(wěn)定劑、以硼氫化鈉為還原劑合成了平均粒徑約5 nm的金納米復(fù)合材料(PPESO3-AuNPs)。PPESO3的熒光可以被金納米粒子猝滅,體系中加入含巰基的生物分子(Cys、Hcy)后,由于巰基更易與金納米粒子作用,從而使得PPESO3的熒光恢復(fù),這為生物硫醇的靈敏檢測提供了一種切實可行的方法,還可用于HeLa細(xì)胞中生物硫醇的檢測。(3)設(shè)計合成了熒光共軛聚電解質(zhì)P2-AgNPs復(fù)合體系用于依替米星的熒光及比色檢測。在最優(yōu)條件下,熒光和比色檢測依替米星的檢測限分別為8 nM和100 nM。
[Abstract]:Gold and silver nanoparticles have unique optical, electrical and chemical properties, and have shown wide application prospects in materials, information and life sciences. Therefore, the preparation and application of gold, silver nanoparticles and their composites have become one of the hot fields of scientific research. In this paper, water soluble conjugated polyelectrolyte (P1PPESO3 / P2) stabilized noble metal nanocomposites were synthesized and used to construct sensitive sensing systems for melamine, bio-mercaptan and etimicin. The specific contents include the following three parts: (1) A novel nano-ratio probe P1-AgNPs was designed and synthesized to achieve a highly sensitive and selective fluorescence and colorimetric detection of melamine. P1 containing pyridine group is not only a good double emission fluorescence probe, but also plays a role in stabilizing silver nanoparticles. With the addition of melamine, due to the excellent binding ability of melamine amino groups to silver nanoparticles, melamine-coated P1-AgNPs were formed, which partially recovered P1 fluorescence at 386 nm, and at the same time due to melamine induced agglomeration. The fluorescence of P1 is further quenched at 488 nm. Therefore, melamine can be detected by ratio fluorescence. In addition, the agglomeration of P1-AgNPs induced by melamine can be used for the determination of melamine by ratio colorimetry. The detection limits of melamine by fluorescence and colorimetry were as low as 0.1 and 0.45 nm. (2) fluorescent conjugated polyelectrolyte PPESO3 was used as stabilizer. The fluorescence of gold nanocomposites (PPESO3-AuNPs. PPESO3) with average diameter of about 5 nm was synthesized with sodium borohydride as reducing agent. The fluorescence of gold nanoparticles could be quenched by gold nanoparticles. Thus, the fluorescence of PPESO3 is restored, which provides a feasible method for the sensitive detection of biological mercaptan. It can also be used for the detection of biological mercaptan in HeLa cells. (3) the fluorescent conjugated polyelectrolyte P2-AgNPs composite system was designed and synthesized for the fluorescence and colorimetric detection of etimicin. Under the optimal conditions, the detection limits of Etimicin for fluorescence and colorimetric detection are 8 nm and 100 nm, respectively.
【學(xué)位授予單位】：湖南師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：O657.3

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