發(fā)布時間：2018-07-25 20:11

【摘要】：建立了蜂蜜中果糖、葡萄糖、蔗糖和麥芽糖的超高效合相色譜-質(zhì)譜(UPC2-MS)快速檢測的方法。樣品先用少量的水溶解,再用正己烷/異丙醇(1:1,V/V)溶解,經(jīng)ACQUITY UPC2BEH(100 mm×3.0 mm,1.7μm)色譜柱分離,以超臨界CO2-甲醇(含0.1%NH3·H2O,V/V)為流動相進(jìn)行梯度洗脫,流速為1.8 m L/min,通過質(zhì)譜檢測器在負(fù)離子電噴霧模式下對目標(biāo)化合物進(jìn)行分析,外標(biāo)法定量。檢測結(jié)果表明:果糖、葡萄糖、蔗糖和麥芽糖在2~150 mg/L范圍線性關(guān)系良好,R20.9990,定量限(LOQ,S/N≥10)為0.22~0.42 mg/L,高、中、低3個濃度水平的加標(biāo)平均回收率在90.0%~107.5%范圍,相對標(biāo)準(zhǔn)偏差(RSD,n=6)均小于4.0%。該方法簡單快速,分離效果好,為蜂蜜中果糖、葡萄糖、蔗糖和麥芽糖的分離測定提供了新的色譜技術(shù)平臺。
[Abstract]:A rapid method for the determination of fructose, glucose, sucrose and maltose in honey by superhigh performance phase chromatography-mass spectrometry (UPC2-MS) was established. The sample was dissolved in a small amount of water, then dissolved with n-hexane / isopropanol (1: 1 V / V). The sample was separated by ACQUITY UPC2BEH (100mm 脳 3.0mm ~ (1.7) 渭 m) column. The supercritical CO _ 2-methanol (containing 0.1%NH3 H _ 2O _ 2O _ V) was used as the mobile phase for gradient elution. At a flow rate of 1.8 mL / min, the target compounds were analyzed by mass spectrometer in anion electrospray mode and quantified by external standard method. The results showed that the linear range of fructose, glucose, sucrose and maltose was 20. 9990 in the range of 2 ~ 150 mg/L. The quantitative limit (LOQN / N 鈮，

本文編號：2144945