【摘要】：糖尿病嚴(yán)重威脅了人類的健康,過去的幾十年里,酶葡萄糖傳感器為人類帶來了福音。目前酶?jìng)鞲衅魅匀淮嬖诿敢兹笔�、重�?fù)穩(wěn)定性和貯存穩(wěn)定性差以及易受外界噪音干擾等缺點(diǎn)。非酶?jìng)鞲衅骺梢圆糠挚朔@些缺點(diǎn),得到人們?cè)絹碓蕉嗟闹匾�。本文以ITO為基底,以電化學(xué)法將ITO電極用納米銅和納米鎳修飾,用于單糖(葡萄糖和果糖)和二糖(乳糖、麥芽糖和蔗糖)的檢測(cè)。研究表明,Cu/Ni/ITO電極對(duì)單糖和二糖都有較好的靈敏度,但是檢測(cè)單糖的靈敏度要高于二糖。其檢測(cè)原理可以總結(jié)為:在電極的催化氧化下醛基轉(zhuǎn)換成羧酸,導(dǎo)致電極表面電導(dǎo)率的變化和氧化峰出現(xiàn)。盡管果糖不是葡萄糖,但它是葡萄糖的同分異構(gòu)體,在堿性環(huán)境下可以異構(gòu)化為葡萄糖從而被檢測(cè)。二糖也能被檢測(cè),這是因?yàn)椴糠侄窃趬A性環(huán)境下也能發(fā)生異構(gòu)化反應(yīng)產(chǎn)生醛基,進(jìn)而被檢測(cè)。進(jìn)一步的實(shí)驗(yàn)表明,納米顆粒的尺寸和形態(tài)影響了其檢測(cè)性能。用磁控濺射法在ITO基底生長(zhǎng)了Au-Pd復(fù)合膜作電極,用電化學(xué)方法在電極上生長(zhǎng)納米銅就可以得到的Cu/Au-Pd/ITO電極,其葡萄糖的檢測(cè)效果明顯好于Cu/ITO電極和Au-Pd/ITO電極,檢測(cè)的靈敏度和響應(yīng)電流強(qiáng)度明顯提高,且響應(yīng)電流與葡萄糖濃度之間呈明顯的線性關(guān)系。形貌分析表明,較之其它電極,Cu/Au-Pd/ITO電極納米顆粒尺寸更小,單位面積分布致密均勻,對(duì)葡萄糖檢測(cè)性能更好。循環(huán)伏安曲線表明,Cu/Au-Pd/ITO電極具有更接近0 V的氧化還原電位,這說明納米銅和納米Au-Pd結(jié)合形成的裸露界面增強(qiáng)了其檢測(cè)葡萄糖的催化活性。這對(duì)于開發(fā)低驅(qū)動(dòng)電壓的非酶葡萄糖傳感器具有重要意義。
[Abstract]:Diabetes is a serious threat to human health. Over the past few decades, enzyme-glucose sensors have brought good news. At present, enzyme sensors still have some disadvantages, such as easy absence of enzyme, poor stability of repeat and storage, and easy to be disturbed by external noise. Non-enzyme sensors can partly overcome these shortcomings, and people pay more and more attention to them. In this paper, ITO electrode was modified with nano-copper and nano-nickel by electrochemical method on ITO substrate, and was used for the detection of monosaccharides (glucose and fructose) and disaccharides (lactose, maltose and sucrose). The results show that Cu- / Ni- / ITO electrode has better sensitivity to both monosaccharides and disaccharides, but the sensitivity of monosaccharides is higher than that of monosaccharides. The detection principle can be summarized as follows: the conversion of aldehyde group to carboxylic acid under the catalytic oxidation of the electrode leads to the change of the surface conductivity of the electrode and the appearance of the oxidation peak. Although fructose is not glucose, it is the isomer of glucose, which can be isomerized to glucose in alkaline environment and detected. Disaccharides can also be detected because some of them can also be isomerized in alkaline environments to produce aldehyde groups, which are then detected. Further experiments show that the size and morphology of nanoparticles affect their detection performance. The Au-Pd composite film was grown on ITO substrate by magnetron sputtering, and the Cu/Au-Pd/ITO electrode was obtained by electrochemical growth of nano-copper on the electrode. The detection effect of glucose was better than that of Cu/ITO electrode and Au-Pd/ITO electrode. The sensitivity and the response current intensity were improved obviously, and the linear relationship between the response current and glucose concentration was obvious. The morphology analysis shows that compared with other electrode Cu-Au-PD / ITO electrode, the size of nanoparticles is smaller, the distribution of unit area is compact and uniform, and the performance of glucose detection is better. The cyclic voltammetry curves show that the Cu / au / PD / ITO electrode has a redox potential closer to 0 V, which indicates that the exposed interface formed by the combination of nano-copper and Au-Pd enhances its catalytic activity for glucose detection. This is of great significance for the development of non-enzymatic glucose sensors with low driving voltage.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.1

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