發(fā)布時(shí)間：2018-07-24 21:53

【摘要】：探討瑪咖中總皂苷含量測(cè)定的方法。以《保健食品檢驗(yàn)與評(píng)價(jià)技術(shù)規(guī)范(2003版)》中"保健食品中總皂苷的測(cè)定"為基礎(chǔ),考察用D101大孔吸附樹(shù)脂代替Amberlite-XAD-2大孔樹(shù)脂純化總皂苷的可行性,并研究樣品在樹(shù)脂中的吸附時(shí)間、樣品總皂苷的濃度兩方面對(duì)檢測(cè)結(jié)果的影響。結(jié)果表明,標(biāo)準(zhǔn)曲線(xiàn)在0~0.176 mg/m L范圍內(nèi)線(xiàn)性關(guān)系良好,線(xiàn)性回歸方程y=3.308 4x-0.006 8(R2=0.999 0),方法檢出限為2μg/m L。兩種樹(shù)脂的總皂苷檢測(cè)結(jié)果無(wú)顯著差異。樣品吸附時(shí)間為60 min,樣品中總皂苷含量在0.08~0.32 mg/m L范圍內(nèi)時(shí),總皂苷的檢測(cè)誤差小。改進(jìn)后的方法平均加標(biāo)回收率99.76%,RSD為2.23%(n=6),精密度試驗(yàn)RSD為2.97%(n=6)。改進(jìn)后的總皂苷檢測(cè)方法靈敏度高,準(zhǔn)確性好,重現(xiàn)性好,可用于測(cè)定瑪咖中總皂苷的含量,瑪咖中總皂苷含量為3.81±0.11 mg/g(n=6)。
[Abstract]:A method for the determination of total saponins in Maca was studied. Based on "determination of Total saponins in Health Foods" (2003 Edition), the feasibility of purifying total saponins by D101 macroporous resin instead of Amberlite-XAD-2 macroporous resin was investigated. The effects of adsorption time and concentration of total saponins on the results were studied. The results show that the standard curve has a good linear relationship in the range of 0 ~ 0.176 mg/m / L, and the linear regression equation is 3.308 4x-0.006 _ 8 (R _ 2N _ (0.999). The detection limit of the method is 2 渭 g / m ~ (L). There was no significant difference in total saponins between the two resins. When the adsorption time was 60 minutes and the content of total saponins was in the range of 0.08 ~ 0.32 mg/m / L, the detection error of total saponins was small. The average recovery of the improved method was 99.76% (RSD = 2.23%) and the precision test (RSD) was 2.97% (n = 6). The improved method has high sensitivity, accuracy and reproducibility. It can be used to determine the content of total saponins in Maca. The content of total saponins in Maca is 3.81 鹵0.11 mg/g (NQ 6).
【作者單位】： 四川大學(xué);瀘州老窖集團(tuán)養(yǎng)生酒業(yè)有限責(zé)任公司;
【基金】：四川大學(xué)-瀘州市人民政府戰(zhàn)略合作科技項(xiàng)目(2013CDLZ-S02) 四川省科技支撐計(jì)劃項(xiàng)目(2015NZ0122)
【分類(lèi)號(hào)】：TS218;O657.3

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 彭維;李雙祁;歐愛(ài)芬;;不同前處理方法對(duì)保健食品中總皂苷含量測(cè)定的影響[J];食品研究與開(kāi)發(fā);2014年08期

2 ;[J];;年期

，

本文編號(hào)：2142768