熒光碳點(diǎn)的制備、材料化及其性能研究
發(fā)布時(shí)間：2018-07-23 11:32
【摘要】：熒光碳點(diǎn)是繼富勒烯、碳納米管及石墨烯之后最熱門的碳納米材料之一。這種納米材料克服了傳統(tǒng)量子點(diǎn)的某些缺點(diǎn),不僅具有優(yōu)良的光學(xué)性能與小尺寸特性,而且具有良好的生物相容性,易于實(shí)現(xiàn)表面功能化,在生化傳感、成像分析、環(huán)境檢測(cè)、光催化技術(shù)及藥物載體等領(lǐng)域具有很好的應(yīng)用潛力。本文主要從熒光碳點(diǎn)的制備與應(yīng)用兩個(gè)方面出發(fā),首先,用檸檬酸和氨基化合物制備了具有高熒光量子產(chǎn)率和其他優(yōu)異熒光性能的碳點(diǎn);其次,將表面官能化的熒光碳點(diǎn)應(yīng)用于金屬離子及小分子的檢測(cè)；最后,將小分子的羅丹明類熒光探針連接到碳點(diǎn)表面,設(shè)計(jì)合成了熒光探針修飾的功能材料。論文的主要內(nèi)容概括如下：1 分別使用檸檬酸與1,2-乙二胺(NCD)和N-氨乙基-3-氨基丙基甲基二甲氧基硅烷(SiCD)制備了兩種碳點(diǎn)。NCD和SiCD的量子產(chǎn)率分別高達(dá)65.5%和55.4%。NCD和SiCD的熒光均可在水相中被痕量的Hg2+猝滅,在此基礎(chǔ)上開發(fā)出兩種具有高選擇性和靈敏性的Hg2+熒光探針。兩種探針被成功用于Hg2+在細(xì)胞中的成像。2首次研究了硅烷官能化SiCD和槲皮素之間的熒光相互作用,并發(fā)現(xiàn)SiCD可以高選擇性和靈敏性地通過熒光和紫外吸收檢測(cè)槲皮素。在此基礎(chǔ)上實(shí)現(xiàn)槲皮素在水相中的快速檢測(cè),碳點(diǎn)的熒光強(qiáng)度與槲皮素濃度的線性范圍為0-40μM,最低檢測(cè)限為79 nm。3用檸檬酸與二乙烯三胺制備了碳點(diǎn)N2CD,其量子產(chǎn)率高達(dá)86%。通過N2CD和槲皮素構(gòu)建一個(gè)基于熒光共振能量轉(zhuǎn)移(FRET)的比率型熒光檢測(cè)系統(tǒng),實(shí)現(xiàn)對(duì)A1~(3+)的檢測(cè)。在FRET比率型熒光檢測(cè)系統(tǒng)中,N2CD為供體,槲皮素-A1~(3+)絡(luò)合物作為受體。此外,基于N2CD/QCT-Al~(3+)的FRET比率型熒光系統(tǒng)被制作成熒光檢測(cè)試紙,通過色度分析實(shí)現(xiàn)對(duì)A1~(3+)的檢測(cè)。4合成了基于羅丹明-三聚氯氰的A1~(3+)熒光探針Rh-1,可通過熒光和顏色高選擇性地檢測(cè)A1~(3+)。通過N2CD表面的氨基,將其固載到N2CD上,設(shè)計(jì)合成了一種熒光檢測(cè)平臺(tái)Rh-CD。Rh-CD不僅保持的Rh-1的檢測(cè)性能,而且具有優(yōu)良的水溶性和生物相容性。
[Abstract]:Fluorescent carbon spot is one of the most popular carbon nanomaterials after fullerene, carbon nanotubes and graphene. This nanometer material overcomes some shortcomings of traditional quantum dots, not only has excellent optical properties and small size characteristics, but also has good biocompatibility, easy to realize surface functionalization, in biochemical sensing, imaging analysis, environmental detection, Photocatalytic technology and drug carrier have good application potential. In this paper, we mainly focus on the preparation and application of fluorescent carbon points. Firstly, we have prepared carbon points with high fluorescence quantum yield and other excellent fluorescence properties using citric acid and amino compounds. The surface functionalized fluorescent carbon spots were applied to the detection of metal ions and small molecules. Finally, the Rhodamine fluorescent probes of small molecules were connected to the carbon point surface, and the functional materials modified by fluorescent probes were designed and synthesized. The main contents of the thesis are summarized as follows: 1. The quantum yields of the two carbon dots. NCD and SiCD were respectively 65.5% and 55.4b. NCD and SiCD were prepared by using citric acid, 1nd-ethylenediamine (NCD) and N-aminoethyl-3-aminopropylmethyldimethoxy silane (SiCD), respectively. Fluorescence can be quenched by trace Hg _ 2 in aqueous phase. On this basis, two Hg2 fluorescent probes with high selectivity and sensitivity were developed. The fluorescence interaction between silane functionalized SiCD and quercetin was studied for the first time by using two probes in Hg2 imaging 2.It was found that SiCD could detect quercetin by fluorescence and UV absorption with high selectivity and sensitivity. On this basis, the rapid detection of quercetin in aqueous phase was realized. The linear range of fluorescence intensity of carbon spot and the concentration of quercetin was 0-40 渭 m, and the lowest detection limit was 79 nm.3. The carbon dot N _ 2CD was prepared by citric acid and diethylenetriamine. The quantum yield was up to 86%. A ratio fluorescence detection system based on fluorescence resonance energy transfer (FRET) was constructed by N2CD and quercetin to detect A1 ~ (3). In the fret ratio fluorescence detection system, N2CD was used as donor, quercetin A 1 ~ (3) complex was used as receptor. In addition, the FRET ratio fluorescence system based on N2CD / QCT-Al3 was made into a fluorescent test paper. The fluorescence probe Rh-1 based on rhodamine-melamine cyanide was synthesized by colorimetric analysis to detect A1 ~ (3), which can be detected selectively by fluorescence and color. A fluorescence detection platform Rh-CD.Rh-CD was designed and synthesized by immobilization of amino group on N2CD surface to N2CD. Rh-CD.Rh-CD not only maintains the detection performance of Rh-1, but also has excellent water solubility and biocompatibility.
【學(xué)位授予單位】：天津工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：O657.3;O613.71
[Abstract]:Fluorescent carbon spot is one of the most popular carbon nanomaterials after fullerene, carbon nanotubes and graphene. This nanometer material overcomes some shortcomings of traditional quantum dots, not only has excellent optical properties and small size characteristics, but also has good biocompatibility, easy to realize surface functionalization, in biochemical sensing, imaging analysis, environmental detection, Photocatalytic technology and drug carrier have good application potential. In this paper, we mainly focus on the preparation and application of fluorescent carbon points. Firstly, we have prepared carbon points with high fluorescence quantum yield and other excellent fluorescence properties using citric acid and amino compounds. The surface functionalized fluorescent carbon spots were applied to the detection of metal ions and small molecules. Finally, the Rhodamine fluorescent probes of small molecules were connected to the carbon point surface, and the functional materials modified by fluorescent probes were designed and synthesized. The main contents of the thesis are summarized as follows: 1. The quantum yields of the two carbon dots. NCD and SiCD were respectively 65.5% and 55.4b. NCD and SiCD were prepared by using citric acid, 1nd-ethylenediamine (NCD) and N-aminoethyl-3-aminopropylmethyldimethoxy silane (SiCD), respectively. Fluorescence can be quenched by trace Hg _ 2 in aqueous phase. On this basis, two Hg2 fluorescent probes with high selectivity and sensitivity were developed. The fluorescence interaction between silane functionalized SiCD and quercetin was studied for the first time by using two probes in Hg2 imaging 2.It was found that SiCD could detect quercetin by fluorescence and UV absorption with high selectivity and sensitivity. On this basis, the rapid detection of quercetin in aqueous phase was realized. The linear range of fluorescence intensity of carbon spot and the concentration of quercetin was 0-40 渭 m, and the lowest detection limit was 79 nm.3. The carbon dot N _ 2CD was prepared by citric acid and diethylenetriamine. The quantum yield was up to 86%. A ratio fluorescence detection system based on fluorescence resonance energy transfer (FRET) was constructed by N2CD and quercetin to detect A1 ~ (3). In the fret ratio fluorescence detection system, N2CD was used as donor, quercetin A 1 ~ (3) complex was used as receptor. In addition, the FRET ratio fluorescence system based on N2CD / QCT-Al3 was made into a fluorescent test paper. The fluorescence probe Rh-1 based on rhodamine-melamine cyanide was synthesized by colorimetric analysis to detect A1 ~ (3), which can be detected selectively by fluorescence and color. A fluorescence detection platform Rh-CD.Rh-CD was designed and synthesized by immobilization of amino group on N2CD surface to N2CD. Rh-CD.Rh-CD not only maintains the detection performance of Rh-1, but also has excellent water solubility and biocompatibility.
【學(xué)位授予單位】：天津工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：O657.3;O613.71
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1 王姍姍;;小分子熒光探針在硫醇檢測(cè)中的最新研究進(jìn)展[J];科技信息;2010年23期
2 向雨秘;龍少波;朱R，
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