發(fā)布時間：2018-07-14 11:46

【摘要】：目的:建立高效液相色譜法同時測定青翹抗毒顆粒中綠原酸、馬錢苷、連翹苷、葛根素、(R,S)-告依春、射干苷、咖啡酸的含量。方法:采用島津InertSustain C18柱(250 mm×4.6 mm,5μm),以甲醇-0.3%磷酸水溶液為流動相進行梯度洗脫;流速為1.0mL·min-1;進樣量為5μL;檢測波長為240 nm(0~25 min,檢測(R,S)-告依春)、327 nm(25~39 min,檢測綠原酸和咖啡酸)、240 nm(39~45 min,檢測葛根素和馬錢苷)、265 nm(45~53 nm,檢測射干苷)、228 nm(53~65 min,檢測連翹苷);柱溫為30℃。結(jié)果:綠原酸、馬錢苷、連翹苷、葛根素、射干苷、咖啡酸、(R,S)-告依春7個成分的進樣質(zhì)量濃度分別在4.56~228μg·mL-1(R2=0.999 9)、4.04~202μg·mL-1(R2=0.999 8)、8.10~405μg·mL-1(R2=0.999 9)、8.06~161μg·mL-1(R2=0.999 9)、2.10~105μg·mL-1(R2=0.999 9)、3.46~173μg·mL-1(R2=0.999 9)、4.58~229μg·mL-1(R2=0.999 9)與峰面積呈良好的線性關(guān)系;各組分分離度良好;加樣回收率(n=6)分別為99.35%(RSD為2.6%)、100.76%(RSD為1.5%)、100.34%(RSD為0.9%)、100.63%(RSD為1.5%)、100.31%(RSD為1.7%)、100.70%(RSD為1.6%)、99.03%(RSD為1.7%)。結(jié)論:本研究建立的含量測定方法符合方法學驗證要求,可用于青翹抗毒顆粒7個指標性成分的同時測定。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of Lv Yuan acid, marganin, suspensin, puerarin, (RHs)-Gouyichun, etoside and caffeic acid in Qingqiao Kangdu granules. Methods: the gradient elution was carried out on InertSustain C18 column (250mm 脳 4.6mm ~ 5 渭 m),) with methanol-0.3% phosphoric acid solution as mobile phase. The flow rate was 1.0 mL min-1, the amount of sample was 5 渭 L, the detection wavelength was 240nm (0 ~ 25 min), the detection wavelength was (RNS) -Gouyichun (2539 min), the Lv Yuan acid and caffeic acid were detected at 240nm (3945 min), the puerarin and marganin were detected at 265 nm (4553 nm), and the column temperature was 30 鈩，

本文編號：2121548