本文選題：高效液相色譜法 + 訶子�。� 參考：《中華中醫(yī)藥雜志》2017年02期

【摘要】：目的:建立雙波長HPLC測定訶子中莽草酸、沒食子酸和鞣花酸含量的方法。方法:采用Agilent C18柱(250mm×4.6mm,5μm),以甲醇-0.1%磷酸為流動相梯度洗脫,流速0.8m L/min,檢測波長214nm(莽草酸)和270nm(沒食子酸和鞣花酸),柱溫25℃。結(jié)果:莽草酸、沒食子酸、鞣花酸分別在各自進樣量范圍內(nèi)與峰面積呈良好線性關(guān)系,相關(guān)系數(shù)均在0.9992以上;各成分的平均回收率均在97.1%-97.5%(n=6),RSD均小于2.0%。結(jié)論:該方法快速、準(zhǔn)確、簡便,可用于訶子中莽草酸、沒食子酸及鞣花酸的含量測定。
[Abstract]:Objective: to establish a dual-wavelength HPLC method for the determination of shikimic acid, Gallic acid and tannic acid in Fructus chebula. Methods: a Agilent C18 column (250mm 脳 4.6 mm ~ (-1) 5 渭 m),) was used with methanol-0.1% phosphoric acid as mobile phase gradient elution at a flow rate of 0.8 mL / min. The detection wavelength was 214nm (shikimic acid) and 270nm (Gallic acid and tannic acid). The column temperature was 25 鈩，

本文編號：2103393