本文選題：超高效液相色譜 + 指紋圖譜　； 參考：《中國實(shí)驗(yàn)方劑學(xué)雜志》2017年07期

【摘要】：目的:研究并建立四川幾種葛根類藥材的超高效液相(UPLC)指紋圖譜,為其藥材鑒別和質(zhì)量控制提供依據(jù)。方法:采用超高效液相色譜-二極管陣列(UPLC-DAD)測定方法,Agilent Zorbax SB-C18色譜柱(2.1 mm×5 mm,1.8μm),乙腈-0.1%乙酸水溶液作為流動(dòng)相,柱溫30℃,流速0.4 m L·min~(-1),檢測波長250 nm,進(jìn)樣量0.5μL;進(jìn)行相似度分析和聚類分析。結(jié)果:建立了野葛、甘葛、峨眉葛的UPLC指紋圖譜,分別確定了29,18,16個(gè)峰,并通過比較對照品與色譜峰的保留時(shí)間,指認(rèn)了野葛7個(gè)峰,甘葛和峨眉葛6個(gè)峰;甘葛和峨眉葛與野葛主要差異為野葛色譜中2,13,14,16,23,25,26,29號峰,甘葛和峨眉葛中無出峰;峨眉葛與甘葛的主要差異為甘葛色譜中6和14號峰,峨眉葛中無出峰;聚類分析得到的樣品之間的相關(guān)性,與相似度分析較一致。結(jié)論:UPLC指紋圖譜技術(shù)快速準(zhǔn)確,結(jié)果可靠,可作為此類藥材指紋圖譜研究的可靠手段。
[Abstract]:Objective: to study and establish the ultrahigh performance liquid chromatography (UPLC) fingerprint of several kinds of Radix Puerariae in Sichuan to provide evidence for their identification and quality control. Methods: an ultra-high performance liquid chromatography-diode array (UPLC-DAD) method was used for the determination of Agilent Zorbax SB-C18 column (2.1 mm 脳 5mm-1 渭 m), acetonitrile-0.1% acetic acid solution) as the mobile phase, and the column temperature was 30 鈩，

本文編號：2067311