本文選題：重金屬離子 + 金納米顆粒　； 參考：《青島科技大學(xué)》2017年碩士論文

【摘要】：很多金屬離子參與人體酶反應(yīng),是維持體系平衡的重要組成物質(zhì)。但一些離子具有生物毒性,進(jìn)入人體后可使人中毒。尤其是重金屬離子,具有致毒劑量低、難以降解、易累積等缺點(diǎn),給人類(lèi)帶來(lái)很大危害。金納米顆粒具有優(yōu)異的光學(xué)性質(zhì)、催化性能、生物相容性、易于和多種物質(zhì)結(jié)合等優(yōu)點(diǎn),受到科學(xué)家們的廣泛關(guān)注。表面等離子體共振效應(yīng)受顆粒粒徑大小、分散程度、周?chē)橘|(zhì)等因素影響。基于金納米顆粒聚集引發(fā)表面等離子體共振變化,使體系顏色由酒紅色向紫色轉(zhuǎn)變等設(shè)計(jì)了一系列金納米顆粒傳感器。金納米顆�？梢院凸Y(jié)合形成穩(wěn)定的金汞齊結(jié)構(gòu),提高催化性能,最大限度的催化顯色劑與底物發(fā)生反應(yīng),根據(jù)體系顏色變化可以實(shí)現(xiàn)對(duì)待測(cè)物的檢測(cè)。金納米顆�？梢院痛判约{米材料結(jié)合,形成的磁性材料集合金納米顆粒及磁性納米顆粒的優(yōu)異性能,可結(jié)合顯色反應(yīng)對(duì)待測(cè)物質(zhì)進(jìn)行可視化分析等。本文基于金納米顆粒比色法檢測(cè)金屬離子,主要包括以下三部分:(1)基于Cr~(3+)可以和修飾在金納米顆粒表面的磷酸根絡(luò)合,導(dǎo)致金納米顆粒團(tuán)聚,根據(jù)表面等離子體共振吸收峰的變化,實(shí)現(xiàn)對(duì)Cr~(3+)及抗壞血酸的可視化檢測(cè);(2)基于汞可以在金納米顆粒表面形成金汞齊結(jié)構(gòu),提高金納米顆粒過(guò)氧化物模擬酶的催化活性,催化TMB和H_2O_2反應(yīng),生成藍(lán)色產(chǎn)物等,實(shí)現(xiàn)對(duì)Hg~(2+)及H_2O_2的比色法可視化檢測(cè);(3)制備了Fe_3O_4@Au復(fù)合納米結(jié)構(gòu),利用適體DNA分子與Au的親和作用,將其修飾在復(fù)合納米結(jié)構(gòu)表面,利用DNA堿基對(duì)Hg~(2+)的特異性識(shí)別與鏈?zhǔn)诫s交技術(shù),使顯色試劑(MB)嵌入至dsDNA,改變體系顏色,實(shí)現(xiàn)對(duì)Hg~(2+)的可視化檢測(cè)。
[Abstract]:Many metal ions participate in the enzyme reaction of human body and are important components to maintain the equilibrium of the system. But some ions are biologically toxic and can be poisoned when they enter the human body. Especially heavy metal ions have the disadvantages of low toxic dose, difficult to degrade and easy to accumulate, which bring great harm to human beings. Gold nanoparticles with excellent optical properties, catalytic properties, biocompatibility, easy to combine with a variety of substances and so on, has been widely concerned by scientists. Surface plasmon resonance (SPR) effect is affected by particle size, dispersion and surrounding media. A series of gold nanoparticle sensors were designed based on the change of surface plasmon resonance induced by gold nanoparticles aggregation and the color change from wine red to purple. Gold nanoparticles can combine with mercury to form stable amalgam structure, improve catalytic performance, and react with substrate to the maximum extent. Gold nanoparticles can be combined with magnetic nanomaterials to form magnetic materials, which can combine the excellent properties of gold nanoparticles and magnetic nanoparticles, and can be used for visual analysis of measuring materials in combination with color reaction. In this paper, the detection of metal ions based on gold nanoparticles colorimetry includes the following three parts: (1) based on Cr ~ (3), the metal ions can be complexed with phosphate modified on the surface of gold nanoparticles, resulting in the agglomeration of gold nanoparticles. According to the change of the surface plasmon resonance absorption peak, the visual detection of Cr ~ (3) and ascorbic acid can be realized. (2) based on the fact that mercury can form gold-amalgam structure on the surface of gold nanoparticles, it can improve the catalytic activity of gold nanoparticles' peroxidase mimetic enzyme. Catalyzing the reaction of TMB and H _ 2O _ 2, producing blue products and so on, realizing the colorimetric visual detection of Hg2 and H _ 2O _ 2. (3) Fe3O _ 4r au composite nanostructures were prepared and modified on the surface of composite nanostructures by the affinity of aptamer DNA molecules to au. By using the specific recognition of DNA base pair Hg2 and chain hybridization technique, the chromogenic reagent (MB) was embedded into dsDNA, the color of the system was changed, and the visual detection of Hg2 was realized.
【學(xué)位授予單位】：青島科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 嚴(yán)進(jìn);;雙氧水氧化1-(2-吡啶偶氮)-2-萘酚動(dòng)力學(xué)光度法測(cè)定痕量硒[J];廣州化工;2016年21期

2 馬哈亞·艾斯江;巴哈爾古麗·別克吐?tīng)栠d;;雙硫腙顯色分光光度法測(cè)定礦樣中的重金屬鉛[J];伊犁師范學(xué)院學(xué)報(bào)(自然科學(xué)版);2016年01期

3 汪洋;;4-(2吡啶偶氮)間苯二酚光度法測(cè)定水樣中微量鈷[J];廣東化工;2015年09期

4 王婷;葛昌華;賈耐靜;陳峰;張泉;李呈宏;;新顯色劑羧基三氮唑偶氮氯膦的合成及其與鑭(Ⅲ)的顯色反應(yīng)[J];冶金分析;2015年02期

5 嚴(yán)露;李潔;張穗忠;李杰;;用鄰二氮雜菲光度法測(cè)定連鑄保護(hù)渣中的鐵含量[J];武漢工程職業(yè)技術(shù)學(xué)院學(xué)報(bào);2014年02期

6 許琳;王科偉;孟雙明;;新顯色劑BABRBBT的合成及其與銅(Ⅱ)的顯色反應(yīng)[J];山西大同大學(xué)學(xué)報(bào)(自然科學(xué)版);2013年02期

7 鐘國(guó)秀;黃清華;高琳;;偶氮氯膦Ⅲ光度法測(cè)定鍶硅鐵合金中鍶[J];冶金分析;2012年09期

8 張輝;;中國(guó)河套地區(qū)的重金屬污染與人群砷中毒[J];AMBIO-人類(lèi)環(huán)境雜志;2004年03期

相關(guān)碩士學(xué)位論文 前1條

1 張見(jiàn)立;深色皮革中六價(jià)鉻離子含量檢測(cè)方法的研究[D];鄭州大學(xué);2006年

，

本文編號(hào)：2064861