本文選題：紫草類 + 羥基萘醌類色素��； 參考：《藥物分析雜志》2016年09期

【摘要】：目的:采用高效液相色譜法建立新疆紫草藥材的特征圖譜,并同時(shí)測(cè)定5種紫草類藥材(軟紫草屬新疆紫草、內(nèi)蒙紫草、滇紫草屬長(zhǎng)花滇紫草、滇紫草屬滇紫草、硬紫草屬硬紫草)6個(gè)主要羥基萘醌色素類成分的含量,比較不同基原紫草的化學(xué)成分差異。方法:以紫草素、乙酰紫草素、β-乙酰氧基異戊酰阿卡寧、異丁酰紫草素、β,β′-二甲基丙烯酰阿卡寧、2-甲基丁基酰紫草素為對(duì)照品,采用YMC Pack ODS-A色譜柱(250 mm×4.6 mm,5μm),以乙腈-0.05%甲酸水溶液(70∶30)為流動(dòng)相,流速1 m L·min~(-1),檢測(cè)波長(zhǎng)275 nm,柱溫30℃;采用CHEMPATTERN化學(xué)計(jì)量學(xué)軟件對(duì)結(jié)果進(jìn)行處理分析。結(jié)果:建立新疆紫草特征圖譜,標(biāo)定10個(gè)特征峰,30 min內(nèi)紫草的主要色譜峰能夠達(dá)到完全分離,對(duì)5種紫草類藥材6個(gè)特征峰成分進(jìn)行含量測(cè)定。紫草素、乙酰紫草素、β-乙酰氧基異戊酰阿卡寧、異丁酰紫草素、β,β′-二甲基丙烯酰阿卡寧、2-甲基丁基酰紫草素質(zhì)量濃度分別在2.13~1 065μg·m L~(-1)(r=0.999 3,n=6)、2.01~1 004μg·m L~(-1)(r=0.999 8,n=6)、2.05~1 026μg·m L~(-1)(r=0.999 7,n=6)、1.92~960μg·m L~(-1)(r=0.999 9,n=6)、2.00~1 001μg·m L~(-1)(r=0.999 8,n=6)和1.87~937μg·m L~(-1)(r=0.999 9,n=6)范圍內(nèi)線性關(guān)系良好,方法的平均回收率(n=6)分別為97.6%(RSD=1.9%)、96.6%(RSD=2.2%)、97.9%(RSD=1.1%)、98.4%(RSD=1.2%)、96.2%(RSD=1.1%)和97.3%(RSD=1.7%);19批新疆紫草中上述6個(gè)羥基萘醌類成分含量分別為0.01%~0.37%、0.02%~2.42%、0.02%~1.42%、0.02%~0.98%、0.05%~1.66%和0.09%~3.15%,2批內(nèi)蒙紫草中均未檢出β-乙酰氧基異戊酰阿卡寧。結(jié)論:所建立新疆紫草特征圖譜專屬性強(qiáng),結(jié)合6個(gè)主要羥基萘醌類成分含量測(cè)定能夠區(qū)分各基原紫草,有助于全面評(píng)價(jià)紫草的質(zhì)量,為規(guī)范紫草藥材的使用提供科學(xué)依據(jù)。
[Abstract]:Objective: to establish the characteristic chromatogram of Rhizoma Arnebia Sinkiang by HPLC, and to determine five kinds of medicinal materials of Rhizoma Arnebia (Arnebia sinensis), Rhizoma Neimongoides, Rhizoma Dianthus, and Dipteryniae at the same time. The contents of six main hydroxyl naphthoquinone pigments were compared and the chemical compositions of different groups were compared. Methods: the contents of porphyrin, acetyl shikonin, 尾 -acetoxy isovaleryl acanine, isobutylol porphyrin, 尾 -dimethacrylyl acanine 2-methyl Ding Ji acyclovidin were used as the reference substance. A YMC pack ODS-A column (250mm 脳 4.6mm ~ 5 渭 m),) was used with acetonitrile-0.05% formic acid solution (70:30) as mobile phase, flow rate 1 mL ~ (-1), detection wavelength 275 nm, column temperature 30 鈩，

本文編號(hào)：2054125