黃花列當(dāng)中麥角甾苷的含量測(cè)定
發(fā)布時(shí)間:2018-06-20 15:30
本文選題:黃花列當(dāng) + 質(zhì)量標(biāo)準(zhǔn); 參考:《內(nèi)蒙古大學(xué)學(xué)報(bào)(自然科學(xué)版)》2016年06期
【摘要】:通過TLC和HPLC法對(duì)黃花列當(dāng)中主要化學(xué)成分麥角甾苷進(jìn)行定性鑒別和含量測(cè)定的研究,結(jié)果證實(shí)列當(dāng)中含麥角甾苷且麥角甾苷在0.0625~2.000μg范圍內(nèi)與峰面積呈良好的線性關(guān)系(R2=0.9999),平均加樣回收率為95.65%,RSD為1.15%.經(jīng)過方法學(xué)論證,TLC和HPLC專屬性強(qiáng),含量測(cè)定方法簡(jiǎn)便、準(zhǔn)確,重現(xiàn)性好,適用于黃花列當(dāng)中麥角甾苷含量的測(cè)定.
[Abstract]:The qualitative identification and content determination of ergosteroside, the main chemical component, were studied by TLC and HPLC. The results showed that there was a good linear relationship between ergosteroside and peak area in the range of 0.06250.000 渭 g. The average recovery was 95.65% and RSD was 1.15%. The method proved that TLC and HPLC were specific, the determination method was simple, accurate and reproducible, which was suitable for the determination of ergosteroside in Huanghua Rhizoma.
【作者單位】: 內(nèi)蒙古化工職業(yè)學(xué)院;內(nèi)蒙古精神衛(wèi)生中心;內(nèi)蒙古大學(xué)生命科學(xué)學(xué)院;
【基金】:內(nèi)蒙古化工職業(yè)學(xué)院科研項(xiàng)目(HYZR1305)
【分類號(hào)】:R284.1;O657.7
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