本文選題：糖蛋白 + 表面分子印跡聚合物　； 參考：《湘潭大學(xué)》2017年碩士論文

【摘要】：糖蛋白作為多種疾病的生物標(biāo)志物,對(duì)臨床診斷具有巨大意義。然而,在沒(méi)有任何預(yù)處理的復(fù)雜生物樣品中測(cè)定低豐度糖蛋白仍然是一個(gè)問(wèn)題。本文提出了基于表面分子印跡和智能材料的方法用于分離和檢測(cè)糖蛋白。主要研究?jī)?nèi)容如下:1、提出了一種基于陽(yáng)離子p H敏感水凝膠和硼酸材料的磁性納米微粒的新型分子印跡技術(shù)用于分離和富集糖蛋白。選用4-乙烯基苯硼酸(VPBA),甲基丙烯酸二甲氨基乙酯(DMA)和N-N-亞甲基雙丙烯酰胺(MBA)合成印跡層。通過(guò)測(cè)試不同p H值下的溶脹比表征了印跡材料受p H調(diào)控的溶脹/收縮性能,同時(shí)也對(duì)該材料對(duì)目標(biāo)糖蛋白的吸附性能進(jìn)行了研究。結(jié)果表明,模板分子的洗脫和吸附能受p H調(diào)控,而且能在10min達(dá)到81.2mg/g的吸附量。2、用修飾苯硼酸的磁性Fe_3O_4納米微粒為載體,首先利用硼酸親和鍵與糖基的共價(jià)鍵結(jié)合力使得目標(biāo)糖蛋白OVA定軌在載體的表面。多巴胺為單體,自聚合反應(yīng)形成包覆在載體表面的印跡層,最后去除模板,得到對(duì)OVA有特異性吸附的磁響應(yīng)分子印跡納米顆粒。同以上體系,做了一系列表征和條件優(yōu)化。結(jié)果表明,制備的印跡粒子對(duì)OVA有較好的吸附能力和選擇性能。3、制備了一種新型共振光傳感器檢測(cè)糖蛋白,具體合成過(guò)程與體系一類(lèi)似。在分子印跡過(guò)程中,印跡層中形成了對(duì)目標(biāo)糖蛋白有特異性識(shí)別的空腔。對(duì)目標(biāo)分子地選擇性捕獲對(duì)應(yīng)著傳感器RLS強(qiáng)度的變化。該傳感器具有效率高(15min)和檢出限低(0.13 n M)的優(yōu)點(diǎn)。
[Abstract]:As a biomarker of many diseases, glycoprotein has great significance in clinical diagnosis. However, the determination of low-abundance glycoproteins in complex biological samples without any pretreatment remains a problem. A method based on surface molecular imprinting and smart materials is proposed for the separation and detection of glycoproteins. A novel molecular imprinting technique based on cationic pH sensitive hydrogels and boric acid materials was proposed for the separation and enrichment of glycoproteins. The imprinted layer was synthesized by using 4-vinylphenylborate (VPBA), dimethylaminoethyl methacrylate (DMA) and N-N- methylene bisacrylamide (N-N- methylene bisacrylamide). The swelling / shrinkage properties of the imprinted materials regulated by pH were characterized by measuring the swelling ratios at different pH values. The adsorption properties of the imprinted materials to the target glycoproteins were also studied. The results show that the elution and adsorption of the template molecule can be regulated by pH, and the adsorption capacity of 81.2mg/g can be reached by 10min. The template molecule can be supported by magnetic Fe _ 3O _ 4 nanoparticles modified with phenylboric acid. Firstly, the covalent binding force of boric acid affinity bond with glycosyl group makes the target glycoprotein OVA orbit on the surface of the carrier. Dopamine was used as monomer and self-polymerized to form imprinted layer coated on the surface of carrier. Finally, the template was removed to obtain magnetically responsive molecularly imprinted nanoparticles which were specifically adsorbed to OVA. In the same system, a series of characterization and conditions were optimized. The results showed that the imprinted particles had good adsorption ability and selectivity to OVA. A new resonance light sensor was prepared for the detection of glycoproteins. The synthesis process was similar to that of the system. In the process of molecular imprinting, a cavity with specific recognition of target glycoprotein was formed in the imprinting layer. The selective capture of the target molecule corresponds to the change of the RLS intensity of the sensor. The sensor has the advantages of high efficiency (15 min) and low detection limit (0.13 nm).
【學(xué)位授予單位】：湘潭大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：O631.3;TQ937

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 譚增琦;周岳;楊剛龍;李想;關(guān)鋒;;凝集素輔助平分型GlcNAc糖基化蛋白質(zhì)組學(xué)分析[J];農(nóng)業(yè)生物技術(shù)學(xué)報(bào);2017年01期

2 Jiangnan Zheng;Zian Lin;Lan Zhang;Huanghao Yang;;Polydopamine-mediated immobilization of phenylboronic acid on magnetic microspheres for selective enrichment of glycoproteins and glycopeptides[J];Science China(Chemistry);2015年06期

3 時(shí)照梅;范超;黃俊杰;白海紅;秦偉捷;蔡耘;錢(qián)小紅;;氧化石墨烯固定化凝集素的制備及在糖蛋白/糖肽富集中的應(yīng)用[J];色譜;2015年02期

4 ;N-glycosylation proteome of endoplasmic reticulum in mouse liver by ConA affinity chromatography coupled with LTQ-FT mass spectrometry[J];Science China(Chemistry);2010年04期

5 孫興權(quán);李靜;耿美玉;管華詩(shī);;糖組學(xué)研究中糖蛋白糖鏈結(jié)構(gòu)分析技術(shù)[J];化學(xué)進(jìn)展;2007年01期

，

本文編號(hào)：2039988