本文選題：綠咖啡豆 + 綠原酸　； 參考：《食品研究與開發(fā)》2017年23期

【摘要】：建立一種高效液相色譜法(HPLC),可同時(shí)定量綠咖啡豆中綠原酸和咖啡堿的含量,并分析不同成熟度綠咖啡豆中綠原酸和咖啡堿含量變化。采用Agilent ZORBAX ODS-C18色譜柱,以0.5%乙酸水-乙腈為流動(dòng)相,梯度洗脫,檢測波長274 nm,流速1.0 m L/min,進(jìn)樣量10μL,柱溫35℃。結(jié)果顯示,綠原酸和咖啡堿的濃度線性范圍分別為5.0 mg/L~100.0 mg/L(r~2=0.999 9)和5.1 mg/L~101.6 mg/L(r~2=0.999 9),檢出限分別為0.25 mg/L和0.05 mg/L,相對標(biāo)準(zhǔn)偏差分別為1.56%和1.30%,回收率范圍分別為98.86%~101.11%(RSD=1.02%)和98.70%~101.19%(RSD=0.83%)。
[Abstract]:A high performance liquid chromatography (HPLC) method was established to determine the content of Lv Yuan acid and caffeine in green coffee beans simultaneously and to analyze the changes of Lv Yuan acid and caffeine contents in green coffee beans with different maturity. Agilent ZORBAX ODS-C18 column with 0.5% acetic acid water-acetonitrile as mobile phase, gradient elution, detection wavelength 274 nm, flow rate 1.0 mL / min, injection volume 10 渭 L, column temperature 35 鈩，

本文編號(hào)：2030684