本文選題：N-琥珀酰殼聚糖 + 溶菌酶��； 參考：《吉林大學(xué)》2017年碩士論文

【摘要】：本文通過琥珀酰酐與殼聚糖的酰化反應(yīng)制備了N-琥珀酰殼聚糖(NSC),利用FTIR、1HNMR證明殼聚糖與琥珀酰酐的反應(yīng)是發(fā)生在殼聚糖的N-位,利用XRD分析表明,N-琥珀酰殼聚糖的結(jié)晶性降低,是殼聚糖水溶性加大的原因。通過p H,EDC/NHS、溶菌酶的添加量等單因素試驗(yàn),確定制備NSC-LSZ(以N-琥珀�；瘹ぞ厶菫檩d體固載溶菌酶的產(chǎn)物)的最佳條件為:p H為6.0,EDC、NHS分別為20 mg,溶菌酶為100 mg。在最佳條件下,酶的固載量可達(dá)89.04%;利用FTIR和CD對NSC-LSZ的二級(jí)結(jié)構(gòu)進(jìn)行表征,發(fā)現(xiàn)以N-琥珀酰殼聚糖固載的溶菌酶的二級(jí)結(jié)構(gòu)發(fā)生變化,主要是因?yàn)槿芫概cN-琥珀酰殼聚糖發(fā)生共價(jià)結(jié)合導(dǎo)致;通過TGA發(fā)現(xiàn),NSC-LSZ的熱穩(wěn)定相對提升;經(jīng)抑菌性試驗(yàn)和LDH細(xì)胞毒性試驗(yàn)發(fā)現(xiàn),NSC-LSZ的抑菌性良好,安全無毒。將NSC-LSZ做成涂膜液用于草莓的室溫保鮮,草莓的保鮮時(shí)間可達(dá)6 d,與不做任何處理的草莓相比,保鮮時(shí)間可以延長3 d;通過對草莓的失重率、質(zhì)構(gòu)、維生素C含量、p H進(jìn)行分析,發(fā)現(xiàn)NSC-LSZ的抑菌保鮮效果良好。通過流涎法制膜,探究溫度、干燥時(shí)間、甘油添加量對膜性能的影響,發(fā)現(xiàn)在在NSC-LSZ溶液中添加5%(wt)的甘油,在40℃條件下干燥7 h,制得的薄膜的阻水、隔氧、透光性、拉伸強(qiáng)度、斷裂伸長率等性能較好。
[Abstract]:N- succinyl chitosan (NSCN) was prepared by acylation of succinic anhydride with chitosan. The reaction between chitosan and succinic anhydride was confirmed by FTIR ~ 1H NMR. XRD analysis showed that the crystallinity of N-succinyl chitosan was decreased. This is the reason for the increase in water solubility of chitosan. The optimum conditions for the preparation of NSC-LSZ (supported lysozyme by N succinylated chitosan) were determined by single factor experiments, such as the amount of lysozyme and the amount of lysozyme. The optimum conditions were as follows: the ratio of% H to 6.0 mg and lysozyme to 100 mg. The optimum conditions for preparation of NSC-LSZ were as follows:% H = 6.0 mg, EDCN / NHS = 20 mg and lysozyme 100 mg, respectively. The secondary structure of NSC-LSZ was characterized by FTIR and CD, and it was found that the secondary structure of lysozyme supported by N-succinyl chitosan changed. It was mainly due to the covalent binding of lysozyme and N-succinyl chitosan; the relative improvement of thermal stability of NSC-LSZ was found by TGA; and the bacteriostatic property of NSC-LSZ was good and safe and non-toxic by bacteriostatic test and LDH cytotoxicity test. Using NSC-LSZ as coating solution to keep strawberry fresh at room temperature, the preservation time of strawberry can be up to 6 days, which can be prolonged by 3 days compared with strawberry without any treatment, and the weight loss rate, texture and vitamin C content of strawberry are analyzed. It was found that NSC-LSZ had a good effect on bacteriostasis and fresh-keeping. The effects of temperature, drying time and glycerol addition on the properties of the film were investigated. It was found that the film was prepared by adding 5% glycerol in NSC-LSZ solution and drying at 40 鈩，

本文編號(hào)：2024065