本文選題：赭曲霉毒素A + 電化學(xué)核酸傳感器��； 參考：《合肥工業(yè)大學(xué)》2017年碩士論文

【摘要】：赭曲霉毒素A是一種由青霉屬和曲霉屬所產(chǎn)生的有毒代謝產(chǎn)物,廣泛存在于谷物等農(nóng)產(chǎn)品中,能侵害人體腎臟和肝臟,并引發(fā)大多數(shù)哺乳動(dòng)物產(chǎn)生癌癥、腎中毒、肝臟中毒以及免疫毒性危害等。因此,建立快速、靈敏、便捷的赭曲霉毒素A檢測(cè)方法對(duì)保護(hù)人類健康具有重要的作用。本研究選取赭曲霉毒素A(OTA)為研究對(duì)象,一方面通過利用核酸適配體以達(dá)到快速檢測(cè)目的;另一方面通過選取合適的樣品預(yù)處理以及合適的納米材料來實(shí)現(xiàn)檢測(cè)范圍的調(diào)節(jié)以及信號(hào)的擴(kuò)增。其具體研究?jī)?nèi)容如下:(1)基于輔助核酸適配體電化學(xué)傳感器對(duì)赭曲霉毒素A的超寬范圍可調(diào)控檢測(cè)利用輔助核酸適配體以及固定電極表面的核酸適配體對(duì)赭曲霉毒素A的特異性識(shí)別實(shí)現(xiàn)超寬范圍可控型檢測(cè)。該電化學(xué)核酸傳感器的檢測(cè)范圍是10-8至100ppb,共有11個(gè)數(shù)量級(jí)的寬度。并且,所有濃度的檢測(cè)信號(hào)響應(yīng)都在同一個(gè)電流區(qū)間內(nèi),實(shí)驗(yàn)操作簡(jiǎn)單,無需信號(hào)放大操作即可實(shí)現(xiàn)寬范圍的檢測(cè)。在最優(yōu)實(shí)驗(yàn)條件下,對(duì)實(shí)際樣品檢測(cè)同樣適用。該設(shè)計(jì)利用核酸適配體的識(shí)別,同樣適用于其他有核酸適配體的目標(biāo)物的檢測(cè)。(2)基于雙重金增強(qiáng)的電化學(xué)核酸傳感器檢測(cè)赭曲霉毒素A利用金納米粒子表面偶聯(lián)核酸探針對(duì)電極表面進(jìn)行不同的自組裝實(shí)現(xiàn)OTA的快速、靈敏檢測(cè)。通過利用金納米粒子的高比表面積作用富集更多的信號(hào)分子實(shí)現(xiàn)檢測(cè)信號(hào)的增強(qiáng);再通過電極表面組裝表面偶聯(lián)核酸探針的金納米粒子使得核酸適配體的自組裝量得到提高,從而實(shí)現(xiàn)檢測(cè)寬度的提高。該電化學(xué)核酸適配體的檢測(cè)范圍在0.001至1000 ppb,與基礎(chǔ)型相比有2個(gè)數(shù)量級(jí)的拓寬。且對(duì)實(shí)際樣品的檢測(cè)可以適用,通過改變核酸適配體序列即可應(yīng)用與其他目標(biāo)物的檢測(cè)。
[Abstract]:Ochratoxin A is a toxic metabolite produced by Penicillium and Aspergillus. It is widely found in agricultural products such as cereals. It can damage human kidneys and liver, and cause cancer and nephrotoxicity in most mammals. Liver poisoning and immune toxicity. Therefore, to establish a rapid, sensitive and convenient method for detection of ochratoxin A plays an important role in the protection of human health. In this study, ochratoxin OTAA was selected as the research object, on the one hand, the aptamer of nucleic acid was used to achieve the purpose of rapid detection. On the other hand, we can adjust the detection range and amplify the signal by selecting the appropriate sample pretreatment and the appropriate nanomaterials. The specific contents of this study are as follows: (1) based on the electrochemical sensor of auxiliary aptamer of nucleic acid for the detection of ochratoxin A in a very wide range, the auxiliary aptamer of nucleic acid and the aptamer of nucleic acid on the surface of fixed electrode can be used for the detection of ochratoxin on the surface of the electrode. The specific recognition of hormone A can achieve a very wide range of controllable detection. The electrochemical nucleic acid sensor has a detection range of 10-8 to 100 ppb, with a total width of 11 orders of magnitude. Moreover, the signal response of all concentrations is in the same current range, so the experiment is simple, and the detection can be carried out in a wide range without signal amplification. Under the optimal experimental conditions, it is also applicable to the actual sample detection. The design utilizes the recognition of aptamers of nucleic acids, It is also suitable for the detection of other targets with aptamer of nucleic acid. (2) Electrochemical nucleic acid sensors based on double gold enhancement for the detection of ochratoxin A using gold nanoparticles surface coupled nucleic acid probes for different electrode surfaces. Self-assembly to achieve the speed of OTA, Sensitive detection. The detection signal was enhanced by enrichment of more signal molecules by using the high specific surface area of gold nanoparticles, and the self-assembly of aptamer was increased by the gold nanoparticles assembled on the surface of the electrode. Thus, the detection width is improved. The detection range of the electrochemical aptamer is from 0.001 to 1000 ppb, which is two orders of magnitude wider than that of the base type. It can be applied to the detection of real samples, and can be applied to the detection of other targets by changing the aptamer sequence of nucleic acid.
【學(xué)位授予單位】：合肥工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：TS207.3;O657.1

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