本文選題：比色/熒光探針 + 靜電紡絲��； 參考：《鄭州大學(xué)》2016年碩士論文

【摘要】：近年來,功能化熒光染料在材料科學(xué)和生命科學(xué)中有著廣泛的應(yīng)用。其中,比色/熒光分子探針因其靈敏度高、選擇性好、無損傷檢測、操作方便等優(yōu)點(diǎn)而成為非常有用的檢測分析技術(shù)。然而,在實際應(yīng)用中分子探針的功能會受到諸如響應(yīng)速度慢、背景干擾大、抗干擾能力差等問題的限制。本論文針對目前熒光探針存在的不足,設(shè)計合成了三種新型的分子探針,具體如下:1、設(shè)計合成出一種鋅離子單核配合物并且可以作為高選擇性裸眼識別焦磷酸根(PPi)的化學(xué)傳感器�；谕�-烯醇式轉(zhuǎn)換過程,PPi的加入使傳感器的顏色從紅色變?yōu)闊o色。此外,將由絡(luò)合物制備的靜電納米纖維浸入到焦磷酸根溶液之后,纖維膜的顏色變化迅速,可實現(xiàn)原位即時檢測PPi。纖維膜的顏色變化迅速是由于納米纖維膜比表面積較大引起的。2、基于1,8-萘酰亞胺設(shè)計合成了一種高選擇性高靈敏度的半胱氨酸(Cys)、同型半胱氨酸(Hcy)、谷胱甘肽(GSH)及苯硫酚熒光分子探針。該探針能夠在0-6.0μM范圍內(nèi)對半胱氨酸(Cys)定量檢測,并且檢出限低至1.6 nM。實驗證實該探針能夠在試紙上檢測Cys以及在細(xì)胞中選擇性識別Cys、Hcy、GSH。同時該探針能快速的識別苯硫酚,表現(xiàn)出了很好的選擇性及抗干擾能力(分析物包括親核試劑、脂肪族硫醇、陰離子及金屬離子)。對苯硫酚的定量檢測的范圍為0-4.0μM,檢出限為10.3 nM。該探針能夠在試紙上及在細(xì)胞中選擇性識別苯硫酚。并且,根據(jù)探針分子與半胱氨酸(Hcy)、同型半胱氨酸(Hcy)、谷胱甘肽(GSH)和苯硫酚的響應(yīng)速率不同,可以成功的將苯硫酚與Cys、Hcy、GSH區(qū)分開來。3、以9,10-苯醌為起始原料設(shè)計合成出一種新穎的水合肼比率/比色/熒光探針。隨著水合肼的加入,該探針分子的顏色和熒光均發(fā)生變化,可以作為一種雙通道的熒光探針,能夠降低檢測環(huán)境,儀器等多方面的影響。在細(xì)胞中,隨著水合肼的加入,該探針的熒光也發(fā)生比率的變化。此外,該探針低毒,表現(xiàn)出很好的生物相容性。
[Abstract]:In recent years, functionalized fluorescent dyes have been widely used in material science and life science. Among them, colorimetric / fluorescent probe has become a very useful detection and analysis technology because of its high sensitivity, good selectivity, no damage detection, easy operation and so on. However, in practical applications, the function of molecular probes is limited by such problems as slow response speed, large background interference and poor anti-jamming ability. In this paper, three novel molecular probes have been designed and synthesized in allusion to the shortcomings of fluorescent probes. As follows: 1, a zinc mononuclear complex has been designed and synthesized and can be used as a highly selective chemical sensor for optically recognized pyrophosphate PPis. Ketone-enol conversion process based on the addition of PPi sensor color from red to colorless. In addition, when the electrostatic nanofibers prepared by the complex were immersed in the pyrophosphate solution, the color of the fiber membrane changed rapidly, and the in situ detection of PPis could be realized. The rapid color change of fiber membrane is caused by the large surface area of nanofiber membrane. Based on the design and synthesis of high selectivity and high sensitivity cysteine cystine, homocysteine cystine, glutathione GSH and benzene based on the design of 1 / 8- naphthalene imide, a kind of high selectivity and high sensitivity cysteine, homocysteine, glutathione (GSH) and benzene were synthesized. Thiophenol fluorescence probe. The probe can be used to detect cysteine in the range of 0-6.0 渭 M, and the detection limit is as low as 1.6 nm. The results showed that the probe could detect Cys on the test paper and selectively recognize cystatin GSH in the cells. At the same time, the probe can recognize phenylthiophenol quickly, showing good selectivity and anti-interference ability. The analytes include nucleophilic reagent, aliphatic mercaptan, anion and metal ion. The range of quantitative detection of p-phenylthiophenol was 0-4.0 渭 M and the detection limit was 10.3 nm. The probe can selectively recognize phenylthiophenol on test paper and in cells. In addition, according to the response rates of probe molecules to cysteine, homocysteine, glutathione, and phenylthiophenol, the response rates of the probe molecules were different from those of cysteine, homocysteine, glutathione and phenylthiophenol. A novel hydrazine hydrate / colorimetric / fluorescent probe was designed and synthesized by separating phenylthiophenol from cystoxy GSH. 3. A new type of hydrazine hydrate / colorimetric / fluorescent probe was designed and synthesized from 9 ~ (10) -benzoquinone. With the addition of hydrazine hydrate, the color and fluorescence of the probe molecule change, which can be used as a two-channel fluorescence probe, which can reduce the detection environment, instruments and other aspects of the impact. The fluorescence of the probe also changed with the addition of hydrazine hydrate. In addition, the probe is low toxic and shows good biocompatibility.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：O657.3

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 曹玉華;探針分子-紅外光譜在分子篩堿性表征上的應(yīng)用[J];石油化工;1999年08期

2 武鵬;齊暑華;劉乃亮;;反氣相色譜法研究增塑劑-探針分子的熱力學(xué)性能[J];中國膠粘劑;2010年04期

3 孫晶;金光勇;李昌立;范薇;董艷麗;翟曉杰;;Ru[(bipy)_3]~(2+)-PMMA溫敏漆的制備及性能研究[J];高校化學(xué)工程學(xué)報;2011年02期

4 ;[J];;年期

相關(guān)會議論文 前2條

1 張銘金;徐君;楊俊;鄧風(fēng);岳勇;葉朝輝;;用固態(tài)NMR探針分子技術(shù)研究介孔MoO_3/ZrO_2的表面酸性[A];第十三屆全國波譜學(xué)學(xué)術(shù)會議論文摘要集[C];2004年

2 付海英;朱光來;徐靜靜;邢兆國;吳國忠;;探針分子在離子液體中的瞬態(tài)光譜研究[A];中國化學(xué)會第27屆學(xué)術(shù)年會第12分會場摘要集[C];2010年

相關(guān)碩士學(xué)位論文 前4條

1 鮑金欣;光化學(xué)傳感器的設(shè)計合成與陽離子識別性能研究[D];西南大學(xué);2015年

2 張文英;幾種氮、氧雜環(huán)類染料的設(shè)計合成及其在檢測方面的應(yīng)用[D];鄭州大學(xué);2016年

3 劉暢;基于半菁可逆加成和共軛體系轉(zhuǎn)化機(jī)制構(gòu)建的溫敏和分子邏輯門器件[D];天津理工大學(xué);2013年

4 李學(xué)召;以殼聚糖為載體的探針分子材料在Hg~(2+)檢測與吸附方面的研究[D];大連理工大學(xué);2012年

，

本文編號：2008623