本文選題：碳量子點(diǎn) + 微波法　； 參考：《延安大學(xué)》2017年碩士論文

【摘要】：碳量子點(diǎn)(Carbon Quantum Dots—CQDs)這一新型納米材料越來越被科研工作者重視,它不僅具有傳統(tǒng)納米材料所具備的所有性質(zhì),而且還具有許多優(yōu)越特性,比如它的低細(xì)胞毒性和良好的生物相容性使得它在生命科學(xué)領(lǐng)域已不可或缺。經(jīng)過科研工作者們多年的努力,CQDs在熒光生物標(biāo)記方面的研究得以大踏步前進(jìn),除此之外,CQDs在離子檢測、藥物檢測中也有著廣泛的應(yīng)用,以CQDs為熒光探針來定量測定離子和藥物的新方法越來越多,這些方法更為簡單、快捷、靈敏度高,而且大多數(shù)已經(jīng)運(yùn)用到實(shí)際樣品檢測。本論文選取廉價易得的碳源,用簡捷的微波法合成熒光性能較好的CQDs,并試圖對合成CQDs的結(jié)構(gòu)、熒光性質(zhì)進(jìn)行分析,探討了其合成作用機(jī)理,同時以CQDs為熒光探針檢測了金屬離子和藥物小分子,探究了CQDs與離子和藥物小分子之間作用機(jī)理,主要內(nèi)容可以分為四部分。1.通過微波法,以D-麥芽糖、D-果糖為碳源合成了CQDs。對合成的CQDs進(jìn)行了結(jié)構(gòu)表征、光學(xué)特性分析及潛在應(yīng)用探討。結(jié)果證明,此類CQDs表面富有羥基和羧基,水溶性好,分布均勻。在弱酸性環(huán)境下具有良好的熒光性能,一些氧化還原劑、金屬離子對其熒光強(qiáng)度有一定的增敏和猝滅作用,但表面活性劑對其熒光性能影響不大,這為其應(yīng)用奠定了良好的基礎(chǔ)。2.以D-麥芽糖為碳源合成的CQDs為熒光探針檢測延河水中痕量的Co2+,并進(jìn)行了相互作用機(jī)理探討。實(shí)驗(yàn)結(jié)果表明,該CQDs對Co2+具有較好的選擇性,體系的熒光猝滅強(qiáng)度與Co2+的濃度在0.03?g/mL~0.6?g/mL及0.6?g/mL~4?g/mL范圍內(nèi)呈良好的線性關(guān)系,檢出限為0.00384?g/mL,回收率在98.8%-103.3%之間,說明該方法的靈敏度及準(zhǔn)確度較高。3.在pH=4.80的檸檬酸-檸檬酸鈉緩沖介質(zhì)中,建立了以CQDs為熒光探針定量測定曲克蘆丁的新方法。結(jié)果表明,當(dāng)曲克蘆丁的濃度在1.0×10-7~3.0×10-5mol/L的范圍內(nèi)時,與CQDs的熒光猝滅強(qiáng)度呈良好的線性,檢出限為4.5×10-9mol/L,且發(fā)現(xiàn)二者之間的作用機(jī)理為動態(tài)猝滅。該方法已成功用于曲克蘆丁藥片的測定,結(jié)果滿意。4.在pH=5.00的B-R的緩沖溶液中,以CQDs為熒光探針定量測定鹽酸雷尼替丁,建立了一種快速、選擇性好的檢測鹽酸雷尼替丁的新方法。實(shí)驗(yàn)發(fā)現(xiàn),鹽酸雷尼替丁濃度在1.0×10-6~7.0×10-5 mol/L和1.0×10-7~1.0×10-6 mol/L范圍內(nèi)與體系熒光猝滅程度呈現(xiàn)良好的線性關(guān)系,檢出限為7.1×10-8 mol/L。對實(shí)際藥品進(jìn)行含量測定,回收率為96.4%~99.0%,并探討了其猝滅機(jī)理為動態(tài)猝滅。
[Abstract]:Carbon Quantum Dot (CQDs), a new nanometer material, has been paid more and more attention by researchers. It not only has all the properties of traditional nanomaterials, but also has many superior properties. For example, its low cytotoxicity and good biocompatibility make it indispensable in life sciences. After many years of efforts by researchers, the study of CQDs in fluorescent biomarkers has made great strides forward. In addition, CQDs have been widely used in ion detection and drug detection. There are more and more new methods for quantitative determination of ions and drugs using CQDs as fluorescent probes. These methods are simpler, faster and more sensitive, and most of them have been applied to the detection of real samples. In this paper, CQDs with good fluorescence properties were synthesized by simple microwave method, and the structure and fluorescence properties of synthesized CQDs were analyzed, and the mechanism of the synthesis was discussed. At the same time, metal ions and drug molecules were detected with CQDs as fluorescence probe, and the mechanism of interaction between CQDs and ions and drug small molecules was explored. The main contents can be divided into four parts. 1. CQDs were synthesized by microwave method using D-maltose and D-fructose as carbon source. The structures, optical properties and potential applications of synthesized CQDs were investigated. The results show that the surface of CQDs is rich in hydroxyl and carboxyl groups, and has good water solubility and uniform distribution. Some oxidizing reductants and metal ions have some sensitizing and quenching effects on the fluorescence intensity, but surfactants have little effect on the fluorescence properties. This has laid a good foundation for its application. CQDs synthesized with D-maltose as carbon source were used as fluorescence probe to detect trace Co _ 2 in Yanhe River water and the interaction mechanism was discussed. The experimental results show that the CQDs have a good selectivity for CO2. The fluorescence quenching intensity of the system is linear with the concentration of CO2 in the range of 0.03g / mL and 0.6g / mL, and the detection limit is 0.00384g / mL and the recovery is between 98.8- 103.3%, and the detection limit is 0.00384g / mL, and the recovery rate is between 98.8- 103.3%, and the detection limit is 0.00384g / mL, and the recovery is between 98.8- 103.3%, and the detection limit is 0.00384g / mL, and the recovery is between 98.8- 103.3%. It shows that the sensitivity and accuracy of this method are high. 3. A new method for quantitative determination of troxerutin with CQDs as fluorescence probe was established in citric acid-sodium citrate buffer medium with pH = 4.80. The results show that when the concentration of troxerutin is in the range of 1.0 脳 10-7, 3.0 脳 10-5 mol / L, the fluorescence quenching intensity with CQDs is linear, the detection limit is 4.5 脳 10-9 mol / L, and the mechanism of the interaction is dynamic quenching. The method has been successfully applied to the determination of troxerutin tablets with satisfactory results. A new rapid and selective method for quantitative determination of ranitidine hydrochloride with CQDs as fluorescence probe was established in the buffer solution of B-R at pH 5.00. It was found that the concentration of ranitidine hydrochloride showed a good linear relationship with the fluorescence quenching in the range of 1.0 脳 10 ~ (-6) ~ 7.0 脳 10 ~ (-5) mol / L and 1.0 脳 10 ~ (-7) mol / L, and the detection limit was 7.1 脳 10 ~ (-8) mol 路L ~ (-1). The content of real medicine was determined. The recovery rate was 96.4% and 99.0. The quenching mechanism was discussed as dynamic quenching.
【學(xué)位授予單位】：延安大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TB383.1;O657.3

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