本文選題：碳點 + 異硫氰酸熒光素　； 參考：《華中農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：碳點(CDs)作為一種新型的納米熒光材料,具備優(yōu)異的光學(xué)性質(zhì)和良好的生物相容性,在生化檢測、生物成像、活體診斷及治療等領(lǐng)域引起研究者的廣泛關(guān)注。CDs在比率型熒光探針及抗病毒等領(lǐng)域的研究仍具有很大的潛力。鑒于此,本文在合成CDs的基礎(chǔ)上,研究了CDs與異硫氰酸熒光素(FITC)的相互作用,并探討了CDs在細(xì)胞成像及抗病毒等方面的應(yīng)用,具體研究內(nèi)容如下:1.利用光譜技術(shù)研究了CDs和FITC在pH為5.1、7.0和9.0條件下的相互作用。當(dāng)CDs和不同濃度的FITC進(jìn)行直接混合后,會導(dǎo)致CDs的熒光強度逐漸地被猝滅,而CDs的熒光壽命保持不變,通過對紫外-可見吸收光譜及熒光壽命的分析,證實了CDs和FITC之間pH依賴的內(nèi)濾效應(yīng)(IFE)。然而,CDs和FITC進(jìn)行化學(xué)偶聯(lián)后,導(dǎo)致了CDs熒光壽命的下降,表明了CDs和FITC之間發(fā)生了熒光共振能量轉(zhuǎn)移(FRET)。該研究不僅揭示了不同pH條件下CDs和FITC之間不同的作用形式,同時也為進(jìn)一步研究納米粒子和熒光團(tuán)之間的相互作用提供了理論指導(dǎo)。2.以溫和的方式合成了兩種不同的CDs,在未經(jīng)進(jìn)一步修飾的情況下可以選擇性地進(jìn)入細(xì)胞質(zhì)和整個細(xì)胞。通過在CDs的水熱合成過程中加入適量的脲,不僅使得CDs的表面電位由負(fù)電位變?yōu)殡娭行?同時制備得到的CDs顯示出不同的熒光。細(xì)胞成像應(yīng)用中,藍(lán)色熒光的CDs(b-CDs)只能進(jìn)入細(xì)胞的細(xì)胞質(zhì),而青色熒光的CDs(c-CDs)可以分布到整個細(xì)胞,包括細(xì)胞核。此外,我們還研究了兩種CDs對偽狂犬病毒(PRV)的抗病毒活性,結(jié)果顯示b-CDs具有更好的抗病毒活性。該研究以一種溫和新穎的方式制備得到了用于細(xì)胞選擇性成像的兩種CDs,研究結(jié)果有望進(jìn)一步促進(jìn)CDs在納米醫(yī)藥領(lǐng)域的應(yīng)用。
[Abstract]:As a new type of nano-fluorescent material, carbon Dot (CDS) has excellent optical properties and good biocompatibility, in biochemical detection, biological imaging, In vivo diagnosis and treatment have attracted wide attention of researchers. CDs still have great potential in the research of ratio fluorescent probe and antivirus. Based on the synthesis of CDs, the interaction between CDs and fluorescein isothiocyanate (FITC) was studied, and the applications of CDs in cell imaging and antivirus were discussed. The interaction between CDs and FITC at pH 5.1 and 9.0 was studied by spectroscopic technique. When CDs are mixed directly with FITC at different concentrations, the fluorescence intensity of CDs is gradually quenched, while the fluorescence lifetime of CDs remains unchanged. The internal filtration effect of pH dependence between CDs and FITC was confirmed. However, chemical coupling of CDs with FITC leads to a decrease in fluorescence lifetime of CDs, which indicates that fluorescence resonance energy transfer occurs between CDs and FITC. This study not only reveals the different forms of interaction between CDs and FITC at different pH, but also provides theoretical guidance for the further study of the interaction between nanoparticles and fluorescent groups. Two different CDss were synthesized in a mild manner, which can selectively enter the cytoplasm and the whole cell without further modification. By adding a proper amount of urea to the hydrothermal synthesis of CDs, not only the surface potential of CDs is changed from negative potential to electric neutral, but also the CDs show different fluorescence. In cell imaging applications, the blue fluorescent CDslb-CDS can only enter the cytoplasm of the cell, while the cyan fluorescence CDstc-CDS can be distributed throughout the cell, including the nucleus. In addition, we also studied the antiviral activity of two CDs against pseudorabies virus (PRV). The results showed that b-CDs had better antiviral activity. Two CDss for cell selective imaging were prepared in a mild and novel way. The results of this study are expected to further promote the application of CDs in the field of nanopharmaceuticals.
【學(xué)位授予單位】：華中農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3

【參考文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 王莉;熒光碳量子點的制備及其發(fā)光機理的研究[D];中國計量學(xué)院;2015年

，

本文編號：1992901