發(fā)布時(shí)間：2018-05-28 15:59

  本文選題：上轉(zhuǎn)換納米顆粒 + 免疫層析試紙條　； 參考：《分析化學(xué)》2017年01期

【摘要】：采用溶劑熱法合成上轉(zhuǎn)換納米顆粒(UCNPs)-Na YF4∶Yb,Er,進(jìn)行表面功能化修飾,將其與己烯雌酚(DES)單克隆抗體偶聯(lián),制備熒光探針,以牛血清白蛋白-己烯雌酚(BSA-DES)偶聯(lián)物和羊抗鼠二抗分別噴涂硝酸纖維膜,形成試紙條檢測(cè)線(T線)和質(zhì)控線(C線),建立了上轉(zhuǎn)換發(fā)光免疫層析試紙條快速定量檢測(cè)DES的方法。實(shí)驗(yàn)結(jié)果表明,此試紙條定量檢測(cè)DES線性范圍為25~10000 ng/m L(y=0.43927x-0.57647,R2=0.996),檢出限為20.84 ng/m L,單個(gè)樣品檢測(cè)時(shí)間為15 min,批內(nèi)和批間變異系數(shù)均小于10%,特異性識(shí)別能力強(qiáng),在37℃存放下7天檢測(cè)值RSD的平均值約為15%。加標(biāo)回收實(shí)驗(yàn)顯示平均回收率為90.1%~115.2%,相對(duì)標(biāo)準(zhǔn)偏差小于5%,與高效液相色譜法有較好的一致性。
[Abstract]:UCNPs-NaYF4: Yb-Er-up-converted nanoparticles were synthesized by solvothermal method. The UCNPs-NaYF4: Yb-Er-were functionalized and coupled with the monoclonal antibody against diethylstilbestrol (DES) to prepare fluorescent probes. Using bovine serum albumin diethylstilbestrol (BSA-DES) conjugate and sheep anti-mouse second antibody respectively sprayed nitric acid fiber membrane to form test strip detection line (T line) and quality control line (C line), a method for rapid quantitative detection of DES by up-conversion immunochromatographic test strip was established. The results showed that the linear range of quantitative detection of DES by this strip was 2510000 ng/m / L ~ 0.3927x-0.57647R20.996, the detection limit was 20.84 ng/m / L, the detection time of a single sample was 15 mins, the coefficient of variation between batches and batches was less than 10 ~ (th), and the specificity recognition ability was strong. The average value of RSD at 37 鈩，

本文編號(hào)：1947324