發(fā)布時間：2018-05-24 21:56

  本文選題：超高效液相色譜法 + 銀黃制劑��； 參考：《中草藥》2017年03期

【摘要】：目的建立同時測定銀黃制劑中10種成分(新綠原酸、綠原酸、隱綠原酸、異綠原酸A、異綠原酸B、異綠原酸C、黃芩苷、漢黃芩苷、黃芩素、漢黃芩素)的超高效液相色譜(UPLC)方法。方法采用Acquiyt UPLC BEH C_(18)色譜柱(100 mm×2.1 mm,1.7μm),以乙腈-0.4%磷酸水溶液為流動相,梯度洗脫,體積流量0.4 m L/min,在326 nm波長處進行檢測,柱溫40℃,進樣量1.0μL。結果銀黃制劑中10種成分在考察線性范圍內線性關系良好(r≥0.999 6),加樣回收率均在97.43%~99.94%,RSD均小于2.0%。11批銀黃制劑樣品中新綠原酸、綠原酸、隱綠原酸、異綠原酸A、異綠原酸B、異綠原酸C、黃芩苷、漢黃芩苷、黃芩素、漢黃芩素質量分數(shù)分別在0.236~3.419、5.279~26.220、0.495~4.714、0.130~2.702、0.310~3.210、0.363~5.036、35.209~133.289、1.493~6.635、1.546~5.393、0.254~0.823 mg/g。結論該方法簡便,準確,快速,分離效果好,重復性好,可用于銀黃制劑的質量控制,并為將來該制劑質量標準提升提供參考。
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of 10 components (new Lv Yuan acid, cryptonic acid, isoLv Yuan acid A, isoLv Yuan acid B, isoLv Yuan acid C, baicalin, Chinese baicalin) in Yinhuang preparation. Methods Acquiyt UPLC BEH Che _ (18) column was used to determine the concentration of acetonitrile-0.4% phosphoric acid solution as mobile phase, and the volume flow rate was 0.4 mL / min. The column temperature was 40 鈩，

本文編號：1930729