發(fā)布時(shí)間：2018-05-22 07:15

  本文選題：長藥八寶 + 1��； 參考：《延邊大學(xué)》2017年碩士論文

【摘要】：衰老是當(dāng)今威脅人類生命健康最主要的原因之一。氧化性自由基的積累加速了機(jī)體的衰老。自由基引起的氧化應(yīng)激與眾多疾病的發(fā)生、發(fā)展有重大關(guān)系[1],使得尋找"完美"抗氧化劑的任務(wù)日益艱巨。在營養(yǎng)物質(zhì)普遍回歸大自然的熱潮中,天然物寶庫既具有豐富的營養(yǎng)物質(zhì),又作為抗氧化劑最具潛力的來源之一,受到了人們的廣泛關(guān)注。天然抗氧化劑具有安全、可靠、生理活性多等優(yōu)點(diǎn)被人們普遍接受,引起人們濃厚的研究興趣。八寶屬植物具備緩解疲勞、清熱、解毒、活血化瘀、消腫止痛之效。近年來,人們對(duì)八寶屬植物的藥用價(jià)值的關(guān)注與日俱增,以期充分開發(fā)八寶屬植物的利用價(jià)值。長藥八寶(Hylotelephiumspectabile)為景天科八寶屬,多年生肉質(zhì)植物草本。地以上的莖粗壯、直立,根肉質(zhì)、短粗,具有顯著的耐旱性�？芍委煹驌p傷,蚊蟲叮咬,皰疹,腳癬等。目前,人們對(duì)長藥八寶的研究主要集中于作為觀賞植物,對(duì)其生物活性研究甚少。本文以長白山地區(qū)長藥八寶的地下根部分為研究對(duì)象,采用浸泡和回流兩種方式對(duì)其活性物質(zhì)進(jìn)行提取。提取物經(jīng)過一系列溶劑依次萃取,得石油醚層組分、三氯甲烷層組分、乙酸乙酯層組分、正丁醇層組分、水層組分。以Vc做陽性對(duì)比,測定各萃取相消除1,1-二苯基-2-苦肼基(DPPH)的能力,來研究長藥八寶的抗氧化活性。結(jié)果表明:回流粗取物和浸泡粗提物的抗氧化活性均以乙酸乙酯層組分三氯甲烷層組分石油醚層組分正丁醇層組分水層組分順序遞減。但回流粗提物的抗氧化活性高于浸泡粗提物的抗氧化活性。尤其是回流提取物中乙酸乙酯組分,即便濃度在0.1mg/mL下,清除率也能到達(dá)96%,其IC50為0.05mg/mL,其抗氧化能力與維生素C(Vc)的相當(dāng)。通過薄層析、柱層析對(duì)長藥八寶的乙酸乙酯層的化學(xué)成分進(jìn)行了分離研究。用核磁共振技術(shù)、液質(zhì)聯(lián)用、紅外光譜方法鑒定了分離出的化合物的結(jié)構(gòu),首次從長藥八寶中得到1,2-丙二醇、反式對(duì)羥基肉桂酸、順式對(duì)羥基肉桂酸、異香草酸、2-甲基吡咯烷酮、對(duì)羥基苯甲酸、沒食子酸。用紫外光譜法,對(duì)反式對(duì)羥基肉桂酸、對(duì)羥基苯甲酸、沒食子酸的含量進(jìn)行了測定。綜上所述,長藥八寶具有良好抗氧化活性,將為其應(yīng)用于食品工業(yè)、化妝品行業(yè)及醫(yī)療保健品等領(lǐng)域,提供數(shù)據(jù)支持。
[Abstract]:Aging is one of the main reasons that threaten human life and health. The accumulation of oxidizing free radicals accelerates the aging of the body. The oxidative stress caused by free radicals is closely related to the occurrence and development of many diseases, which makes the task of searching for "perfect" antioxidants increasingly arduous. In the upsurge of nutrient returning to nature, natural treasure house is not only rich in nutrients, but also one of the most potential sources of antioxidants. Natural antioxidants are widely accepted by people because of their safety, reliability and many physiological activities. Bubao plants have the effect of relieving fatigue, clearing heat, detoxifying, promoting blood circulation and removing blood stasis, detumescence and pain. In recent years, people pay more and more attention to the medicinal value of the genus Euphorbia, in order to fully exploit the utilization value of the genus Euphorbia. Hylotelephium spectabile is a perennial fleshy plant herb. Stems above ground are stout, erect, rooting fleshy, short and tolerant to drought. Can be used to treat injuries, mosquito bites, herpes, tinea pedis and so on. At present, the research on the long medicine Bubao is mainly focused on the ornamental plant, but its biological activity is very little. In this paper, the underground roots of Changbaobao in Changbai Mountain area were studied. The active substances were extracted by soaking and reflux. The extract was extracted by a series of solvents in turn to obtain the components of petroleum ether layer, trichloromethane layer, ethyl acetate layer, n-butanol layer and water layer. The antioxidant activity of Babao was studied by using VC as positive contrast to determine the ability of each extraction phase to eliminate 1 ~ (-1) diphenyl-2-picrylhydrazinyl DPPHs. The results showed that the antioxidant activity of crude refluxing extract and soaking crude extract was decreased in the order of ethyl acetate layer, trichloromethane layer, petroleum ether layer, n-butanol layer, water layer component. However, the antioxidant activity of reflux crude extract was higher than that of soaking crude extract. In particular, the ethyl acetate fraction in the reflux extract, even if the concentration was at 0.1mg/mL, the clearance rate could reach 96mg / mL, its IC50 was 0.05mg / mL, and its antioxidant capacity was equal to that of vitamin C / Vc. The chemical constituents of ethyl acetate layer of Bubao were separated by thin chromatography and column chromatography. The structures of the compounds were identified by NMR, GC-MS and FTIR. For the first time, 1m2-propanediol, trans-p-hydroxycinnamic acid, cis-p-hydroxycinnamic acid were obtained from Babao. Isovanilic acid 2-methyl pyrrolidone, p-hydroxybenzoic acid, Gallic acid. The contents of trans-p-hydroxycinnamic acid, p-hydroxybenzoic acid and Gallic acid were determined by UV spectrophotometry. To sum up, Bubao has good antioxidant activity, which will provide data support for its application in food industry, cosmetics industry and medical and health products.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R284;O652.6

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