本文選題：近紅外光譜 + 單倍體鑒別　； 參考：《光譜學(xué)與光譜分析》2016年01期

【摘要】：使用JDSU公司的MicroNIR1700型微型近紅外光譜儀,研究了適合進行單籽粒玉米單倍體鑒別的光譜測量方法�；诮t外光譜定性分析技術(shù),比較了漫反射和漫透射兩種情況下玉米單倍體鑒別的效果。光譜數(shù)據(jù)經(jīng)過預(yù)處理后,采用PLS+OLDA特征提取算法,應(yīng)用SVM建立玉米單倍體鑒別模型,分別統(tǒng)計漫反射和漫透射實驗條件下,鑒別模型的正確識別率。在微型光譜儀內(nèi)置光源漫反射的光譜測量方式下,不分胚面朝向,玉米單倍體籽粒平均識別率低于60%,不能有效鑒別玉米單倍體和多倍體。而采用外置光源對籽粒進行漫透射光譜測量方式,獲得了平均正確識別率為93.2%的鑒別效果,并且模型穩(wěn)定性好。實驗結(jié)果表明,漫反射光譜僅能獲得玉米籽粒表層信息,因此玉米籽粒胚面朝向嚴重影響漫反射光譜鑒別單倍體種子的效果;而漫透射光譜可以實現(xiàn)分析光程縱深信息全累加,能夠得到樣品內(nèi)部的信息,因此對胚面朝向不敏感,能夠有效地對隨機擺放的玉米單倍體和多倍體進行識別。近紅外方法能快速、無損地鑒別單倍體,并且微型光譜儀采集速度快,成本低,為實現(xiàn)實用化的自動鑒別提供了條件。
[Abstract]:The method of spectral measurement suitable for haploid identification of single grain maize was studied by using MicroNIR1700 miniature near infrared spectrometer made by JDSU Company. Based on the qualitative analysis of near infrared spectroscopy, the effects of haploid identification in maize under two conditions of long reflection and diffuse transmission were compared. After the spectral data was preprocessed, the haploid identification model of maize was established by SVM using PLS OLDA feature extraction algorithm, and the correct recognition rate of the identification model was calculated under the condition of long reflection and diffuse transmission experiments, respectively. The average recognition rate of maize haploid grains was lower than 60 and could not be used to identify haploid and polyploidy effectively under the method of diffuse reflectance of light source in micro spectrometer, and the average recognition rate of haploid grains was lower than 60% without differentiation of embryo face orientation. The results showed that the average correct recognition rate was 93.2% and the stability of the model was good. The results show that the diffuse reflectance spectrum can only obtain the surface information of maize grains, so the effect of the diffuse reflectance spectrum on the identification of haploid seeds is seriously affected by the orientation of the embryo surface, and the diffuse transmission spectrum can achieve the total accumulation of the depth information of the light path analysis. The information inside the sample can be obtained, so it is insensitive to the orientation of embryo, and can be used to identify haploid and polyploid maize. The near infrared method can identify haploid quickly and undamaged, and the acquisition speed of miniature spectrometer is fast and the cost is low, which provides the condition for realizing practical automatic identification.
【作者單位】： 中國科學(xué)院半導(dǎo)體研究所高速電路與神經(jīng)網(wǎng)絡(luò)實驗室;中國石油大學(xué)(華東)信息與控制工程學(xué)院;中國農(nóng)業(yè)大學(xué)國家玉米改良中心;中國農(nóng)業(yè)大學(xué)信息與電氣工程學(xué)院;
【基金】：National Key Scientific Instrument and Equipment Development Project(2014YQ470377) the China Scholarship Council(201404910237)
【分類號】：S513;O657.3

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本文編號：1920104