發(fā)布時間：2018-05-20 14:43

  本文選題：試紙條 + 膠體金��； 參考：《山東師范大學》2017年碩士論文

【摘要】：近年來,食品安全問題越來越受到社會的重視,其中對食品中致病菌、農獸藥殘留等有害物質的檢測方法研究是監(jiān)督食品安全的重要保障。對食品中有害物質的檢測方法有多種,膠體金(Gold nanoparticles,GNPs)試紙條檢測方法是常用的快速檢測方法之一。膠體金試紙條檢測方法包括膠體金免疫層析技術和膠體金免疫滲濾技術,本論文主要研究基于膠體金免疫層析技術的檢測方法。膠體金免疫層析技術是一種以GNPs作為顯色物質的免疫層析檢測技術,通常根據抗原上表位的數目分為雙抗夾心法和競爭法,載體可以為硝酸纖維素膜(Nitrocellulose membrane,NC),玻璃等,廣泛應用于食品、醫(yī)療檢測等領域。傳統(tǒng)膠體金免疫層析試紙條通常通過視覺檢測法得出檢測結果,但這種檢測方法靈敏度一般較低。GNPs具有良好的光熱效應,在532 nm激光照射下,GNPs分子被激發(fā),產生大量熱量。本論文制作了鼠傷寒沙門氏菌膠體金免疫層析式試紙條,在此基礎上利用GNPs的光熱性質,用熱像儀進行光熱檢測,得出定量分析結果。由于熱像儀價格相對仍較高,本論文進一步采用價格更低廉的紅外溫度傳感器進行光熱檢測,建立了一種基于GNPs光熱效應的層析式試紙條的傳感器快速檢測方法,研制了一種新型的光熱檢測裝置。論文內容如下:第一章主要概述了試紙條檢測方法的分類、GNPs的制備方法和性質,介紹了GNPs的標記技術和光熱效應機理,重點介紹了膠體金試紙條的視覺檢測方法和膠體金試紙條讀卡器檢測方法及其研究進展,以及基于GNPs光熱效應的應用及其研究進展。第二章建立了一種基于GNPs光熱效應的鼠傷寒沙門氏菌層析式試紙條檢測方法。制備了GNPs,并與抗體組成金標抗體復合物,制作了鼠傷寒沙門氏菌免疫層析式試紙條,對其制作過程進行了優(yōu)化。優(yōu)化了光熱檢測的激光照射時間,分別用視覺檢測法及熱像儀光熱效應檢測法檢測,得到了鼠傷寒沙門氏菌的最低檢測限,分別為1.57×10~7 CFU·mL~(-1)和1.7×10~6 CFU·m L-1,光熱效應檢測法與視覺檢測法相比,靈敏度提高了約9倍。第三章選用雙抗夾心法商品化鼠傷寒沙門氏菌試紙條和競爭法商品化鹽酸克倫特羅試紙條雙抗夾心,利用視覺檢測法及熱像儀光熱效應檢測法進行了定量檢測,確定了商品化膠體金試紙條采用視覺檢測法及熱像儀檢測法檢測鼠傷寒沙門氏菌及鹽酸克倫特羅的最低檢測限。鼠傷寒沙門氏菌試紙條視覺檢測法和熱像儀檢測法的檢測限分別為1.13×10~7 CFU·m L-1及1.2×10~6 CFU·mL~(-1),熱像儀檢測方法與視覺檢測方法相比,靈敏度提高了約9倍。鹽酸克倫特羅試紙條視覺檢測法和熱像儀檢測法的檢測限分別為5 ng·mL~(-1)及0.5ng·mL~(-1),熱像儀檢測方法與視覺檢測方法相比,靈敏度提高了約10倍。第四章研制了一種小型紅外溫度傳感器裝置,將第二章和第三章中測試的自制和商品化鼠傷寒沙門氏菌試紙條和商品化鹽酸克倫特羅試紙條用紅外溫度傳感器進行光熱檢測。分別對激光照射距離、傳感器測量距離及激光照射時間進行了優(yōu)化,確定了膠體金免疫試紙條方法采用視覺檢測法及紅外傳感器光熱效應檢測法檢測鼠傷寒沙門氏菌及鹽酸克倫特羅的最低檢測限。自制鼠傷寒沙門氏菌試紙條視覺檢測法和傳感器檢測法檢測限分別為1.57×10~7 CFU·mL~(-1)及1.25×10~6 CFU·mL~(-1),傳感器檢測方法與視覺檢測方法相比,靈敏度提高了約12倍。商品化鼠傷寒沙門氏菌試紙條視覺檢測法和傳感器檢測法檢測限分別為1.13×10~7 CFU·mL~(-1)及1.0×10~6 CFU·m L-1,傳感器檢測方法與視覺檢測方法相比靈敏度提高了約11倍。鹽酸克倫特羅試紙條視覺檢測法和傳感器檢測法檢測限分別為5 ng·m L-1及0.3 ng·mL~(-1),傳感器檢測方法與視覺檢測法相比靈敏度提高了約16倍。第五章總結了本論文建立的基于GNPs光熱效應的快速檢測方法以及研制的檢測裝置,該方法能夠實現(xiàn)待測物的快速檢測,與試紙條視覺檢測法相比靈敏度得到顯著提高,且具有簡單快捷、不需借助大型儀器及應用廣泛的特點。
[Abstract]:In recent years, the problem of food safety has been paid more and more attention by the society. The research on detection methods of harmful substances such as pathogens in food, veterinary drug residues and other harmful substances is an important guarantee for supervision of food safety. There are many kinds of detection methods for harmful substances in food. The testing method of colloidal gold (Gold nanoparticles, GNPs) test strip is a common fast method. Colloidal gold immunochromatography and colloidal gold immunofiltration are one of the detection methods of colloidal gold test paper. This paper mainly studies the detection method based on colloidal gold immunochromatography. Colloidal gold immunochromatography is an immunochromatographic detection technique using GNPs as a chromogenic substance, usually based on the epitope of the antigen. The number is divided into double resistance sandwich method and competition method. The carrier can be widely used in food, medical inspection and other fields, such as Nitrocellulose membrane, NC, glass and so on. Traditional colloidal gold immunochromatography paper is usually obtained by visual detection, but the sensitivity of this detection method is generally lower than that of.GNPs. Good photothermal effect, under 532 nm laser irradiation, GNPs molecules are excited to produce a large amount of heat. In this paper, a colloidal gold immunochromatographic test paper for Salmonella typhimurium was made. Based on the photothermal properties of GNPs, the thermograph was used to detect the heat and heat, and the result of quantitative analysis was obtained. Further using the cheaper infrared temperature sensor to detect the light and heat, a fast detection method of the sensor based on the GNPs photothermal effect is established, and a new type of light and heat detection device is developed. The contents of the paper are as follows: the first chapter mainly outlines the classification of test paper detection method and the preparation method of GNPs. The marking technology and the photothermal effect mechanism of GNPs are introduced. The visual detection method of colloidal gold strip and the detection method of colloidal gold test paper reader and the research progress, and the application and research progress based on the GNPs photothermal effect are introduced. A kind of Salmonella typhimus based on the GNPs photothermal effect is established in the second chapter. The detection method of bacterial chromatography test paper strip was prepared. The GNPs was prepared and the antibody composition of the antibody was made up of the gold standard antibody complex. The preparation process of Salmonella typhimurium was made. The process of its production was optimized. The laser irradiation time of the photothermal detection was optimized. The mouse injury was detected by the visual detection method and the thermo thermal effect detection method respectively. The minimum detection limits for Salmonella Cambrian are 1.57 * 10~7 CFU. ML~ (-1) and 1.7 x 10~6 CFU. M L-1. The sensitivity of the photothermal effect detection is about 9 times higher than that of the visual inspection. The third chapter uses the double anti sandwich method to commercialize the Salmonella typhimurium test paper and the competitive method commercialized clenbuterol test paper double resistance sandwich. The quantitative detection method of visual detection and thermograph photothermal effect detection was carried out, and the minimum detection limit for the detection of Salmonella typhimurium and clenbuterol hydrochloride by visual detection and thermography was determined. The detection limits for the test paper and the thermograph test method of Salmonella typhimurium were respectively. For 1.13 * 10~7 CFU. M L-1 and 1.2 x 10~6 CFU. ML~ (-1), the sensitivity of the thermal imager is about 9 times higher than that of the visual detection method. The detection limit of the clenbuterol test paper and the thermal imager detection method is 5 ng mL~ (-1) and 0.5ng. A small infrared temperature sensor device was developed in Chapter fourth. The light and heat detection of the self-made and commercialized Salmonella typhimurium test paper and the commercialized clenbuterol test paper in the second and third chapters and the infrared temperature sensor for the commercialized clenbuterol test paper were tested in the second and third chapters. The distance of the laser irradiation, the measurement distance and the excitation of the sensor were measured respectively. The light irradiation time was optimized. The minimum detection limit for the detection of Salmonella typhimurium and clenbuterol was determined by the colloidal gold immunoassay strip method and the infrared sensor photothermal effect detection method. The test limits of the self-made test paper for Salmonella typhimurium and the detection method of sensor detection were 1.57 x 10~7 C, respectively. FU. ML~ (-1) and 1.25 x 10~6 CFU. ML~ (-1), the sensitivity of the sensor detection method is about 12 times higher than that of the visual detection method. The detection limits of the visual detection method and the sensor detection method for Salmonella typhimurium commercialization are 1.13 * 10~7 CFU mL~ (-1) and 1 x 10~6. The sensitivity increases about 11 times. The detection limits of the clenbuterol test method and the sensor detection method are 5 ng. M L-1 and 0.3 ng. ML~ (-1) respectively. The sensitivity of the sensor detection method is improved by about 16 times compared with the visual detection method. The fifth chapter summarizes the rapid detection method based on the photothermal effect based on GNPs in this paper and the fifth chapter. The developed detection device can realize the rapid detection of the object to be measured. Compared with the test paper vision detection method, the sensitivity is greatly improved, and it is simple and quick, without the need of large instruments and wide applications.
【學位授予單位】：山東師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TS207.3;O652

【參考文獻】

相關期刊論文 前10條

1 楊偉群;許玉麗;許曉蕓;管月清;;基于圖像識別原理的金標試紙檢測儀樣機研制[J];分析試驗室;2015年12期

2 楊玉東;劉公召;李冬至;徐菁華;楊林梅;;金納米棒:性能、制備、修飾、生物成像技術及應用[J];科學通報;2015年09期

3 袁云燕;詹鵬飛;肖俊銳;;對比分析膠體金試紙法與化學發(fā)光免疫法測定人絨毛膜促性腺激素的結果[J];中國現(xiàn)代藥物應用;2014年09期

4 李淑群;曹碧云;�；�;鄧安平;;膠體金免疫層析法快速檢測牛奶、奶粉、飼料中的三聚氰胺[J];分析化學;2013年07期

5 陳貝特;黃立華;郭凱;黃惠杰;;一種高性能金標條閱讀儀的研制[J];中國激光;2013年07期

6 張洋;賈睿;何培民;;免疫膠體金試紙條快速檢測貝類體內的腹瀉性貝毒[J];生物技術通報;2013年06期

7 李超輝;羅薇;徐波;劉文娟;許恒毅;熊勇華;;膠體金免疫層析試紙條定量檢測豬尿中克倫特羅[J];食品科學;2013年12期

8 張洪才;劉春燕;劉國艷;陳小連;葉雨丹;王藝如;柴春彥;;基于免疫膠體金試紙條檢測鹽酸克倫特羅的便攜式光電型傳感器[J];分析化學;2012年06期

9 李懷明;許恒毅;熊勇華;;免疫層析試紙條技術及其在食源性致病菌檢測中應用的研究進展[J];食品科學;2011年17期

10 于建華;刁幼林;;金標滲濾法的影響因素分析及解決辦法[J];中國誤診學雜志;2010年18期

，

本文編號：1915100