本文選題：流動注射 + 化學發(fā)光�。� 參考：《重慶師范大學》2017年碩士論文

【摘要】：流動注射化學發(fā)光是一種利用體系內被檢測的物質的濃度,與化學發(fā)光強度在一定環(huán)境中呈一定的線性關系,而建立起來的一種在線的測定方法,其特點是靈敏度很高,檢測快速,操作簡單。但是其對物質的檢測沒有特異性,難以對復雜的混合物中的物質進行測定,所以,研究一種對物質特異性識別的檢測方法就受到了廣泛的關注。分子印跡法是利用目標分子合成的一種對目標分子有特異性識別的印跡聚合物,將此印跡柱與流動注射化學發(fā)光分析儀連用,就能精確的測定出目標分子,從而彌補了流動注射化學發(fā)光分析儀無特異性識別的缺點,由此建立起來的分子印跡流動注射化學發(fā)光分析方法特異性識別強,靈敏度高,操作簡單,應用范圍廣。本文主要研究了核苷、磺胺類藥物對在酸性條件下的高錳酸鉀-甲醛發(fā)光體系的增強現(xiàn)象,并建立了對核苷、磺胺類物質檢測的體系,以及各項優(yōu)化參數(shù)。具體介紹如下:1.在酸性條件下,利用KMnO_4-HCHO發(fā)光體系,結合流動注射化學發(fā)光分析儀,對尿嘧啶核苷進行了測定,結果顯示回歸方程:ΔI=86.58×105C(mol/L)+0.6,相關系數(shù)為R=0.9955,檢出限是8×10-6 mol/L,對濃度為1×10-3 mol/L的尿嘧啶核苷平行測定11次,相對標準偏差為4.3%,運用此方法對尿嘧啶核苷成功進行了檢測。2.將流動注射系統(tǒng)與化學發(fā)光聯(lián)用來測定鳥嘌呤核苷,可以快速的測定出其含量,實驗所得其線性方程:ΔI=49.67×105C(mol/L)-302.77,相關系數(shù)為R=0.9993,檢出限是1.5×10-7 mol/L,對濃度為1×10-6 mol/L的鳥嘌呤核苷平行測定11次,相對標準偏差為2.7%。3.胞嘧啶核苷為目標分子,合成對其有專一性識別的分子印跡聚合物(MIP),在酸性條件下,利用HCl-KMnO_4-HCHO發(fā)光體系,結合流動注射化學發(fā)光(FI-CL)分析方法,創(chuàng)建對胞嘧啶核苷測定有特異性識別的MIP-FI-CL分析方法,其線性范圍是8×10-5mol/L-1×10-3mol/L,檢出限是4×10-5mol/L,利用此方法測定復雜樣品中的胞嘧啶核苷的含量,結果令人滿意。4.在酸性條件下,利用HCl-KMnO_4-HCHO發(fā)光體系,結合流動注射化學發(fā)光(FI-CL)分析方法,以磺胺二甲基嘧啶為目標分子,合成對其有專一性識別的分子印跡聚合物(MIP),創(chuàng)建對磺胺二甲基嘧啶測定有特異性識別的MIP-FI-CL分析方法,其線性范圍是1×10-4 mol/L-1.5×10-3 mol/L,檢出限是6×10-5 mol/L,利用此方法測定復雜樣品中的磺胺二甲基嘧啶的含量,結果令人滿意。
[Abstract]:Flow injection chemiluminescence (FIA) is a kind of on-line determination method, which utilizes the concentration of the substance detected in the system and has a certain linear relationship with the chemiluminescence intensity in a certain environment. Rapid detection, simple operation. However, the detection of substances is not specific, so it is difficult to determine the substances in complex mixtures. Therefore, the study of a detection method for the specific identification of substances has been paid more and more attention. Molecular imprinting is a kind of imprinted polymer synthesized by target molecule, which can be used in combination with flow injection chemiluminescence analyzer to determine the target molecule accurately. Thus, the shortcomings of flow injection chemiluminescence analyzer without specific recognition were remedied, and the molecular imprinted flow injection chemiluminescence analysis method was established with strong specificity, high sensitivity, simple operation and wide application range. In this paper, the enhancement of nucleoside and sulfonamides on the luminescent system of potassium permanganate and formaldehyde under acidic conditions was studied, and the system for the detection of nucleoside and sulfanilamides was established, as well as the optimized parameters. The details are as follows: 1. Under acidic conditions, the uracil nucleoside was determined by using KMnO_4-HCHO luminescence system and flow injection chemiluminescence analyzer. The results showed that the regression equation was: 螖 Ion 86.58 脳 105Cnmol / L) 0.6, the correlation coefficient was RX 0.9955, the detection limit was 8 脳 10-6 mol / L, 11 times for uracil with concentration of 1 脳 10-3 mol/L, and the relative standard deviation was 4.30.The method was used to detect uracil successfully. The flow injection system and chemiluminescence were used to determine the content of guanine nucleoside. The linear equation was obtained as follows: 螖 Ion 49.67 脳 10 ~ (-5) Con mol / L ~ (-302.77), the correlation coefficient is R _ (0.9993), the detection limit is 1.5 脳 10 ~ (-7) mol / L, and the parallel determination of guanine nucleoside with concentration of 1 脳 10 ~ (-6) mol/L is 11 times. The relative standard deviation is 2.7. 3. Cytosine nucleoside was used as the target molecule to synthesize the molecularly imprinted polymer (MIP) with specific recognition. In acidic conditions, HCl-KMnO_4-HCHO luminescence system was used in combination with flow injection chemiluminescence (FI-CLL) analysis method. A MIP-FI-CL method for the determination of cytosine nucleoside was established. The linear range was 8 脳 10-5mol/L-1 脳 10 -3 mol / L and the detection limit was 4 脳 10 ~ (-5) mol / L. the method was used to determine the content of cytosine nucleoside in complex samples with satisfactory results. In acidic conditions, sulfadimethazine was used as the target molecule, using HCl-KMnO_4-HCHO luminescence system and flow injection chemiluminescence (FI-CLL) analysis method. The molecularly imprinted polymer (MIP) was synthesized and a MIP-FI-CL method for the determination of sulfadimethylpyrimidine was established. The linear range is 1 脳 10 ~ (-4) mol/L-1.5 脳 10 ~ (-3) mol / L and the detection limit is 6 脳 10 ~ (-5) mol 路L ~ (-1). The method is used to determine the content of sulfadimethylpyrimidine in complex samples with satisfactory results.
【學位授予單位】：重慶師范大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：O657.3;TQ460.72

【參考文獻】

相關期刊論文 前10條

1 姚春紅;馮娟;鄧建平;;化學發(fā)光免疫分析法檢測乙型肝炎標志物的應用分析[J];國際檢驗醫(yī)學雜志;2017年03期

2 金鑫;;化學發(fā)光法檢測降鈣素原與血培養(yǎng)結果的相關性分析[J];標記免疫分析與臨床;2017年01期

3 張保平;劉珊;韓艷秋;;化學發(fā)光法測量人附睪蛋白4的不確定度的應用與分析[J];標記免疫分析與臨床;2017年01期

4 黃良欣;;化學發(fā)光法檢測血清胰島素、C肽的臨床效果評價[J];臨床醫(yī)學研究與實踐;2017年02期

5 羅潔;涂小珂;王丙濤;葛麗雅;孫春菊;;流動注射分析法測定甜型葡萄酒中總二氧化硫[J];食品工業(yè);2017年01期

6 吳迎春;任傳清;郝亮;王建偉;聶峰;;毛細管電泳-電致化學發(fā)光法同時測定鼻炎康片中馬來酸氯苯那敏和鹽酸麻黃堿[J];分析科學學報;2016年06期

7 張旭輝;唐書澤;鄧小玲;朱曜;吳事正;;流動注射化學發(fā)光法在線檢測飲用水中的3-硝基丙酸[J];食品與機械;2016年11期

8 柴川;周禮明;崔小兵;戴貞麗;于生;單晨嘯;文紅梅;;基于液質聯(lián)用技術對不同產地淡豆豉核苷和氨基酸類成分的分析[J];中國實驗方劑學雜志;2016年23期

9 黃志波;王紀陽;程雪梅;梁志剛;何健安;;超高效液相色譜-質譜/質譜法同時測定蔬菜中7種磺胺的殘留量[J];熱帶農業(yè)科學;2016年06期

10 王慧敏;唐玉霞;;流動注射分析法與紫外分光光度法測定土壤硝態(tài)氮含量的比較[J];河北農業(yè)科學;2016年02期

，

本文編號：1904217