本文選題：高效毛細(xì)管電泳 + 沙棘��； 參考：《山西師范大學(xué)》2017年碩士論文

【摘要】：進(jìn)入21世紀(jì),隨著人類生活水平的提高,人們對于健康和養(yǎng)生的關(guān)注度也逐漸提高,在確保食品安全的前提下,人們更多的希望通過食療來調(diào)節(jié)自身的機(jī)能,在滿足基本營養(yǎng)的同時(shí)也能祛除疾病。因此對于食品和藥物中有效成分的分析研究成為當(dāng)下的熱點(diǎn)。在眾多的分析檢測技術(shù)中,毛細(xì)管電泳以其較高的分離效率,較短的分析時(shí)間,較低的成本消耗等優(yōu)勢廣受分析檢測實(shí)驗(yàn)的歡迎,是發(fā)現(xiàn)并解決各自研究領(lǐng)域中一些新問題的得力工具。本論文主要應(yīng)用高效毛細(xì)管電泳-紫外吸收檢測器對食品和藥物中有效成分進(jìn)行檢測研究,具體內(nèi)容如下:第一章:主要對高效毛細(xì)管電泳及其應(yīng)用作出簡單的綜述。在技術(shù)方面包括高效毛細(xì)管電泳的發(fā)展情況,基礎(chǔ)分離理論,分離模式,進(jìn)樣技術(shù),信號檢測方式及其特點(diǎn)等。在了解技術(shù)的基礎(chǔ)上,本章對高效毛細(xì)管電泳技術(shù)在不同領(lǐng)域的應(yīng)用進(jìn)行簡要概括,并對本論文研究的意義做出總結(jié)。第二章:應(yīng)用高效毛細(xì)管區(qū)帶電泳法,對3個(gè)產(chǎn)地沙棘果粉中的蘆丁、山柰酚、異鼠李素和槲皮素4種活性物質(zhì)進(jìn)行同時(shí)分離測定。考察了影響電泳分離效率的各種條件:緩沖溶液20 mmol/L Na2B4O7-H3BO3,1.5 mg/m L的β-環(huán)糊精,pH 9.55,檢測波長370 nm,分離電壓25 kV,進(jìn)樣時(shí)間5 s。蘆丁、山柰酚、異鼠李素和槲皮素4種活性物質(zhì)在11 min內(nèi)實(shí)現(xiàn)較好分離,線性范圍分別為0.01~0.51、0.05~0.93、0.02~0.65、0.03~0.81mg/mL,相關(guān)系數(shù)r在0.9971~0.9991之間,檢出限分別為5.05×10-5、2.10×10-5、3.75×10-5、1.31×10-5mg/mL(RSN=3),各物質(zhì)線性關(guān)系良好。日內(nèi)及日間精密度的范圍是0.08%~4.72%,平均回收率在95.51%~104.66%之間,RSD%不大于3.92%(n=3)。在優(yōu)化條件下,對4種活性物質(zhì)標(biāo)準(zhǔn)品的混合物進(jìn)行測定,并用相同的方法對3個(gè)產(chǎn)地沙棘果粉中活性物質(zhì)的提取液進(jìn)行檢測。結(jié)果表明:生產(chǎn)地不同的沙棘中,4種被檢測化合物的含量各不相同。沙棘中被測4種化合物總量最高的產(chǎn)自新疆烏魯木齊,最低的產(chǎn)自甘肅武威,而槲皮素含量以產(chǎn)自山西呂梁的最高,山柰酚和異鼠李素以產(chǎn)自新疆烏魯木齊最高。通過應(yīng)用該方法進(jìn)行檢測,可以實(shí)現(xiàn)沙棘果粉中多種黃酮類物質(zhì)的快速分離。第三章:利用高效毛細(xì)管電泳法,對蘋果中根皮苷,表兒茶素,蘆丁,綠原酸4種酚類化合物進(jìn)行分離與測定。優(yōu)化了實(shí)驗(yàn)條件,最佳實(shí)驗(yàn)條件:緩沖溶液10 mmol/L Na2B4O7-H3BO3,pH 9.2,檢測波長280 nm,分離電壓25 kV,進(jìn)樣時(shí)間5 s。其線性范圍分別為0.005~0.120、0.005~0.550、0.004~0.450、0.020~0.600 mg/m L,相關(guān)系數(shù)r在0.9977~0.9992之間,最低檢出限分別為3.41×10-5、5.67×10-5、3.78×10-5、3.89×10-5mg/m L(S/N=3)。之后,又進(jìn)行了日內(nèi)及日間精密度和平均回收率的試驗(yàn),其范圍分別為0.13%~4.59%和96.72%~104.55%,RSD%≤3.74%(n=3)。在優(yōu)化條件下,對所檢測酚類化合物標(biāo)準(zhǔn)品的混合物進(jìn)行測定,并用相同的方法測定蘋果皮和蘋果肉樣品提取液。實(shí)驗(yàn)結(jié)果表明,本方法操作簡便、準(zhǔn)確可靠,可作為蘋果中多種酚類物質(zhì)質(zhì)量檢測的一種手段。第四章:以高效毛細(xì)管電泳-紫外檢測技術(shù)為基礎(chǔ),研究了決明子中5種蒽醌類化合物的毛細(xì)管電泳遷移情況,通過優(yōu)化實(shí)驗(yàn)條件,分析各種影響因素,并對所測對照品和供試品進(jìn)行定性和定量分析,最終確定最佳分離條件為:檢測波長284 nm,分離電壓25 kV,進(jìn)樣時(shí)間5 s,運(yùn)行緩沖溶液Na2B4O7-H3BO3濃度20 mmol/L,pH 8.50,β-環(huán)糊精濃度為5 mmol/L,在該條件下,橙黃決明素,大黃素,大黃素甲醚,大黃酸,大黃酚在10 min內(nèi)實(shí)現(xiàn)分離,線性范圍依次為0.005~0.400、0.004~0.350、0.004~0.400、0.002~0.350、0.003~0.300 mg/mL,線性相關(guān)系數(shù)r大于0.9952,檢出限分別為5.03×10-5、5.66×10-5、1.67×10-5、5.19×10-5、3.36×10-5mg/mL(S/N=3),平均回收率在96.00%~103.64%(n=3)之間,RSD%在1.39%~4.56%之間。實(shí)驗(yàn)結(jié)果表明,該方法線性關(guān)系良好,操作簡便,分析時(shí)間短,檢測成本低,可以為決明子中蒽醌類成分的分析提供一定的參考。
[Abstract]:In twenty-first Century, with the improvement of human living standard, people's attention to health and health is also increasing. On the premise of ensuring food safety, people are more hoping to regulate their own functions through diet therapy, and can also eliminate diseases while satisfying basic nutrition. Therefore, the analysis of the effective components in food and medicine is studied. In many analysis and detection techniques, capillary electrophoresis is popular with the advantages of high separation efficiency, short analysis time and low cost consumption. It is a useful tool for discovering and solving some new problems in their respective fields. This paper mainly applies high performance capillary electricity. The specific content of the active components in food and drugs is detected by swimming and ultraviolet absorption detector. The specific contents are as follows: Chapter 1: a brief overview of high performance capillary electrophoresis and its applications, including the development of high performance capillary electrophoresis, basic separation theory, separation mode, sampling technique, signal detection method and its application. On the basis of understanding the technology, this chapter briefly summarizes the application of high performance capillary electrophoresis in different fields and summarizes the significance of this study. The second chapter: using high performance capillary zone electrophoresis, 4 active substances of rutin, kaempferol, isamamenin and quercetin in 3 areas of sea buckthorn fruit powder are entered. The conditions affecting the electrophoresis separation efficiency were investigated. The conditions of affecting the electrophoresis separation efficiency were: 20 mmol/L Na2B4O7-H3BO3,1.5 mg/m L of buffer solution, beta cyclodextrin, pH 9.55, detection wavelength 370 nm, separation voltage 25 kV, 5 s. rutin, kaempferol, ISO rhamnin and quercetin in 11 min to achieve a better separation and linear range in 11 min. The detection limit is 5.05 x 10-5,2.10 x 10-5,3.75 x 10-5,1.31 x 10-5mg/mL (RSN=3), and the correlation coefficient r is between 0.9971~0.9991 and 0.9971~0.9991. The linear relationship between each substance is good. The range of intra day and day precision is 0.08%~4.72%, the average recovery is between 95.51%~104.66% and RSD% is not more than 3.92% (n=3). Under the optimum conditions, the mixture of 4 kinds of active substances was measured and the extracts of the active substances in the 3 species of seabuckthorn fruit powder were detected by the same method. The results showed that the content of the 4 kinds of compounds detected in the different Seabuckthorn production was different. The highest total of the 4 compounds in the sand spines was produced from Xinjiang. Urumqi, the lowest production from Wuwei in Gansu, and the highest content of quercetin from Lvliang in Shanxi, kaempferol and ISO rhamnin is the highest in Urumqi of Xinjiang. The rapid separation of Flavonoids from sea buckthorn fruit powder can be realized by this method. The third chapter: by high performance capillary electrophoresis, it can be used in apple. The separation and determination of 4 kinds of phenolics, such as glucoside, epicatechin, rutin and chlorogenic acid, optimized the experimental conditions. The optimum experimental conditions were as follows: the buffer solution was 10 mmol/L Na2B4O7-H3BO3, pH 9.2, the detection wavelength was 280 nm, the separation voltage was 25 kV, and the linear range of the sampling time was 0.005~0.120,0.005~0.550,0.004~0.450,0.020~0.600 mg/m L in the linear range of the sampling time. The minimum detection limit is 3.41 x 10-5,5.67 x 10-5,3.78 x 10-5,3.89 x 10-5mg/m L (S/N=3) between 0.9977~0.9992 and R. After that, the intraday and daytime precision and average recovery are tested, the range is 0.13%~4.59% and 96.72%~104.55%, RSD% < 3.74% (n=3). The mixture of the quasi product was determined and the same method was used to determine the extract of apple skin and apple meat. The experimental results showed that the method was simple, accurate and reliable, and could be used as a means to detect the quality of various phenols in apple. The fourth chapter was based on high performance capillary electrostroke UV detection technology, and studied 5 in cassia seed. The capillary electrophoresis migration of anthraquinone compounds is made by optimizing the experimental conditions and analyzing various influencing factors. The qualitative and quantitative analysis of the tested control products and the tested products is carried out. The optimum separation conditions are determined as follows: the detection wavelength is 284 nm, the separation voltage is 25 kV, the injection time is 5 s, the Na2B4O7-H3BO3 concentration of the buffer solution is 20 mmol/L, pH 8.50, the concentration of beta cyclodextrin is 5 mmol/L. Under this condition, orange cassia, emodin, emodin, chrysophanol and chrysophanol are separated in 10 min, the linear range is 0.005~0.400,0.004~0.350,0.004~0.400,0.002~0.350,0.003~0.300 mg/mL in turn, the linear correlation coefficient is more than 0.9952, and the detection limit is 5.03 * 10-5,5.66 x 10-5,1., respectively. 67 * 10-5,5.19 x 10-5,3.36 x 10-5mg/mL (S/N=3), the average recovery rate is between 96.00%~103.64% (n=3) and RSD% in 1.39%~4.56%. The experimental results show that the method has a good linear relationship, simple operation, short analysis time and low detection cost, which can provide some reference for the analysis of anthraquinones in cassia seed.

【學(xué)位授予單位】：山西師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O658.9;TS207.3;TQ460.72

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 任強(qiáng);吳昊;聶其婷;劉偉;;HPLC-DAD法同時(shí)測定苦蕎麥保健品中蘆丁槲皮素山奈酚含量[J];濟(jì)寧醫(yī)學(xué)院學(xué)報(bào);2016年05期

2 付陽;王宇坤;劉明博;張明陽;吳迪;李春芳;柳沛;張林;孫知遙;馮旭東;李亞娜;王玉珍;;UPLC-ESIMS法同時(shí)測定沙棘黃酮中5種主要成分含量[J];內(nèi)蒙古農(nóng)業(yè)大學(xué)學(xué)報(bào)(自然科學(xué)版);2016年03期

3 黃娜娜;竇立雯;孫蓉;;基于功效及物質(zhì)基礎(chǔ)的決明子藥理與毒理作用研究進(jìn)展[J];中國藥物警戒;2016年05期

4 許耀宗;;決明子的現(xiàn)代藥理藥性與臨床新用研究[J];北方藥學(xué);2016年04期

5 楊美麗;張少杰;楊金蕊;張艷慧;段康飛;;決明子的特征圖譜研究及質(zhì)量分析[J];中國藥業(yè);2015年22期

6 楊巍;高文民;馬海仲;王柏松;和陽;呂春晶;魏瀟;張素敏;;前處理對‘澳洲青蘋’蘋果果實(shí)中有機(jī)酸組成與含量的影響[J];食品科學(xué);2015年10期

7 Hao-Yue Xie;Zuo-Rong Wang;Zhi-Feng Fu;;Highly sensitive trivalent copper chelate-luminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples[J];Journal of Pharmaceutical Analysis;2014年06期

8 殷東生;周志軍;郭樹平;;光照對沙棘幼苗生長、生物量分配及光合特性的影響[J];經(jīng)濟(jì)林研究;2014年03期

9 周逸芝;劉訓(xùn)紅;陳菲;張奉蘇;;高效液相色譜法同時(shí)測定龍柴方中槲皮素、山柰素、異鼠李素[J];中成藥;2013年10期

10 王玉寶;;紫外分光光度法測定決明子中蒽醌類成分含量[J];中醫(yī)藥臨床雜志;2013年08期

相關(guān)博士學(xué)位論文 前1條

1 賈振寶;決明子中蒽醌化合物組成和功能的研究[D];江南大學(xué);2006年

相關(guān)碩士學(xué)位論文 前1條

1 鄭言博;蒽醌類化合物抗菌及抗腫瘤生物活性的研究[D];湖北工業(yè)大學(xué);2012年

，

本文編號：1894100